FastQCFastQC Report
Thu 2 Feb 2017
SRR4062541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256391
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG112520.8955810730895081No Hit
TCCATGTACTCTGCGTTGATACCAC100210.7976020203901493No Hit
GAGTACATGGAAGCAGTGGTATCAA50580.40258168038453No Hit
CATGTACTCTGCGTTGATACCACTG45430.3615912562251719No Hit
GTATCAACGCAGAGTACTTTTTTTT34910.27785936066081335No Hit
GCTTCCATGTACTCTGCGTTGATAC30750.2447486491068465No Hit
CCCATGTACTCTGCGTTGATACCAC29320.23336684201017038No Hit
GTACATGGGAAGCAGTGGTATCAAC27600.21967683627151102No Hit
GCGTTGATACCACTGCTTCCATGTA26940.21442369453458357No Hit
CATGGAAGCAGTGGTATCAACGCAG25650.20415619023058906No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25590.20367863189086838No Hit
TATCAACGCAGAGTACTTTTTTTTT25010.1990622346069018No Hit
GTATCAACGCAGAGTACATGGAAGC20900.16634948833603552No Hit
ACTCTGCGTTGATACCACTGCTTCC19490.15512686735259962No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18970.15098802840835376No Hit
ACGCAGAGTACATGGAAGCAGTGGT18390.14637163112438722No Hit
CAGTGGTATCAACGCAGAGTACATG17610.14016337270801846No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16160.1286223794981021No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14780.11763853768452656No Hit
TATCAACGCAGAGTACATGGAAGCA14000.11143027926815777No Hit
GTACATGGGGTGGTATCAACGCAAA13630.1084853361732136No Hit
GTATCAACGCAGAGTACATGGGAAG13550.1078485917202527No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13510.10753021949377224No Hit
GCGCAAGACGGACCAGAGCGAAAGC13400.106654695870951No Hit
GAATAGGACCGCGGTTCTATTTTGT13320.1060179514179901No Hit
GAATAACGCCGCCGCATCGCCAGTC13060.10394853194586717No Hit
GGTATCAACGCAGAGTACTTTTTTT12740.10140155413402357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG902.3046596E-914.7693491
GTCTAGT602.5813304E-514.2418751
AACGGAC655.447422E-513.1547315
GGACATA604.1107836E-412.6594441
GCGTTAT1052.0079824E-812.6594431
CGTCTTA1001.440294E-712.35082915
GGTTCTA2850.012.3336713
AGGCCCG2550.012.29494310
ATCGCCA2400.012.27263616
CGCATCG2400.012.27116913
CGACCAT1551.8189894E-1212.25888710
CGAACGA701.0890828E-412.2160816
CGCCCGA550.003064506712.09268516
GCGAGAA550.003065421712.0922029
GCATCGC2600.012.05754114
CGGTCCA2450.012.02121510
AACCGAC802.8598573E-511.87721717
AACGCCG2400.011.8743775
TAGGGTC1201.0017175E-811.8739044
ATTTGCG1459.640644E-1111.79483516
Produced by FastQC (version 0.11.5)