##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062541_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1256391
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.106619674926037	32.0	32.0	32.0	32.0	32.0
2	30.836266735435068	32.0	32.0	32.0	32.0	32.0
3	30.834328644506368	32.0	32.0	32.0	32.0	32.0
4	30.869242934723346	32.0	32.0	32.0	32.0	32.0
5	30.726368622506847	32.0	32.0	32.0	32.0	32.0
6	34.3479227406118	36.0	36.0	36.0	32.0	36.0
7	34.20802918836572	36.0	36.0	36.0	32.0	36.0
8	34.188693647121	36.0	36.0	36.0	32.0	36.0
9	34.32254608636961	36.0	36.0	36.0	32.0	36.0
10	34.05915833526346	36.0	36.0	36.0	32.0	36.0
11	34.346326899826565	36.0	36.0	36.0	32.0	36.0
12	34.14899820199285	36.0	36.0	36.0	32.0	36.0
13	34.25361849933659	36.0	36.0	36.0	32.0	36.0
14	34.157046651878275	36.0	36.0	36.0	32.0	36.0
15	34.120079656731065	36.0	36.0	36.0	32.0	36.0
16	34.10727074612919	36.0	36.0	36.0	32.0	36.0
17	34.031646995242724	36.0	36.0	36.0	32.0	36.0
18	34.053736456246504	36.0	36.0	36.0	32.0	36.0
19	34.04112812014731	36.0	36.0	36.0	32.0	36.0
20	34.016768665168726	36.0	36.0	36.0	32.0	36.0
21	33.96638307660593	36.0	36.0	36.0	32.0	36.0
22	33.91975030066277	36.0	36.0	36.0	32.0	36.0
23	33.86569706405092	36.0	36.0	36.0	32.0	36.0
24	33.85385441315641	36.0	36.0	36.0	32.0	36.0
25	33.35150999967367	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	43.0
5	135.0
6	220.0
7	63.0
8	190.0
9	205.0
10	138.0
11	42.0
12	90.0
13	71.0
14	289.0
15	297.0
16	447.0
17	633.0
18	920.0
19	1316.0
20	1977.0
21	2852.0
22	4289.0
23	5943.0
24	8005.0
25	10908.0
26	15005.0
27	19360.0
28	25774.0
29	33928.0
30	43902.0
31	60259.0
32	86865.0
33	124534.0
34	279588.0
35	528098.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.24209956537659	16.955646124209956	11.343193288013628	25.45906102239982
2	16.135197177807072	20.30329659133602	36.685483408791335	26.876022822065575
3	19.49311330726812	24.22568484863922	28.03652844461991	28.244673399472752
4	12.59887963610861	15.303387441002581	35.04484560169209	37.05288732119672
5	14.412905886746241	36.64858434792579	33.51304180272198	15.425467962605987
6	34.85032000057335	34.12383866906518	17.01684770966866	14.008993620692815
7	29.938727608258116	30.58052265915649	20.85953385831287	18.621215874272526
8	28.807120044803003	32.174515025815104	19.972149484614103	19.046215444767792
9	27.276044372070196	14.691396776938252	18.355980382471692	39.67657846851986
10	15.69586057686561	27.074433115385077	31.7743060121047	25.45540029564462
11	37.31756470849798	21.304537344499188	21.204449448839227	20.173448498163594
12	24.13170922978345	24.066119206480263	28.776247963760515	23.025923599975773
13	30.240408253360606	19.398489290441443	24.94431858297069	25.41678387322726
14	23.89821931617597	19.556602157221352	24.918098106952595	31.627080419650078
15	25.71403300759213	26.53794789415642	23.549543261109303	24.198475837142148
16	26.048955088777813	25.613731456979234	24.315152268509554	24.0221611857334
17	23.95916757312423	25.779334361315975	26.142315829737385	24.119182235822407
18	25.433151943485427	23.990401321053657	27.364364067595616	23.212082667865307
19	24.800419523461514	26.768370290024286	25.270391000010363	23.160819186503844
20	24.860702924282318	23.877495075194922	26.95079657526823	24.31100542525453
21	26.016911331069988	25.11229060074276	23.9940069175473	24.876791150639953
22	24.699642249048228	25.409245406949676	24.893583440148458	24.997528903853635
23	23.512702039866383	24.81498691657614	25.249852348201596	26.422458695355882
24	23.695468869297436	26.577415914984915	25.14198137943896	24.585133836278693
25	25.112948176531486	24.69430871890588	24.66219150942118	25.530551595141453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	229.0
1	229.0
2	551.5
3	874.0
4	874.0
5	874.0
6	2382.5
7	3891.0
8	3891.0
9	3891.0
10	3939.5
11	3988.0
12	3988.0
13	3988.0
14	3852.0
15	3716.0
16	3716.0
17	3716.0
18	6124.0
19	8532.0
20	8532.0
21	8532.0
22	13191.5
23	17851.0
24	17851.0
25	17851.0
26	26697.0
27	35543.0
28	35543.0
29	35543.0
30	43378.5
31	51214.0
32	51214.0
33	51214.0
34	62278.0
35	73342.0
36	73342.0
37	73342.0
38	83363.0
39	93384.0
40	93384.0
41	93384.0
42	111710.0
43	130036.0
44	130036.0
45	130036.0
46	167368.0
47	204700.0
48	204700.0
49	204700.0
50	188597.0
51	172494.0
52	172494.0
53	172494.0
54	153473.0
55	134452.0
56	134452.0
57	134452.0
58	123671.0
59	112890.0
60	112890.0
61	112890.0
62	100353.5
63	87817.0
64	87817.0
65	87817.0
66	72301.0
67	56785.0
68	56785.0
69	56785.0
70	42939.0
71	29093.0
72	29093.0
73	29093.0
74	22761.0
75	16429.0
76	16429.0
77	16429.0
78	13826.5
79	11224.0
80	11224.0
81	11224.0
82	7773.0
83	4322.0
84	4322.0
85	4322.0
86	3238.0
87	2154.0
88	2154.0
89	2154.0
90	1448.0
91	742.0
92	742.0
93	742.0
94	440.5
95	139.0
96	139.0
97	139.0
98	344.5
99	550.0
100	550.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010426690417234763
2	0.0050143625670670995
3	0.004377618114106197
4	0.015281866871061636
5	0.03724955049821274
6	0.04871095065150896
7	0.06765409812709579
8	0.08898503730128599
9	0.09447695820807375
10	0.1198671432698897
11	0.11891202659044836
12	0.12949790312092335
13	0.11811609602424722
14	0.12225493496849309
15	0.11198743066449855
16	0.11174865149463822
17	0.1198671432698897
18	0.13013464757388427
19	0.13005505451726412
20	0.12090185300595117
21	0.12822441421500153
22	0.15019209784215265
23	0.1392878490851972
24	0.13124895036656584
25	0.12830400727162167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1256391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.09408225672727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.38725897095993	41.35606398969296
2	13.001547890797555	13.546074105759734
3	3.5436223131118614	5.538052567980704
4	1.4423351255330938	3.0054849868515214
5	0.7135718668460926	1.8586435763783393
6	0.41292871925331087	1.290668560016821
7	0.2814383860893756	1.026289210459835
8	0.17474298893364967	0.7282460511436742
9	0.1384104795049993	0.6489330214073847
>10	0.7092720660230291	6.956509602714048
>50	0.08426255429727365	3.1170290783569445
>100	0.09419376905488173	10.212423125489934
>500	0.01104551972741787	3.8802358155347307
>1k	0.004909119878852387	4.7310378358462
>5k	1.5340999621413708E-4	0.4042228648070082
>10k+	3.0681999242827417E-4	1.7000856075601989
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	11252	0.8955810730895081	No Hit
TCCATGTACTCTGCGTTGATACCAC	10021	0.7976020203901493	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5058	0.40258168038453	No Hit
CATGTACTCTGCGTTGATACCACTG	4543	0.3615912562251719	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3491	0.27785936066081335	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3075	0.2447486491068465	No Hit
CCCATGTACTCTGCGTTGATACCAC	2932	0.23336684201017038	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2760	0.21967683627151102	No Hit
GCGTTGATACCACTGCTTCCATGTA	2694	0.21442369453458357	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2565	0.20415619023058906	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2559	0.20367863189086838	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2501	0.1990622346069018	No Hit
GTATCAACGCAGAGTACATGGAAGC	2090	0.16634948833603552	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1949	0.15512686735259962	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1897	0.15098802840835376	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1839	0.14637163112438722	No Hit
CAGTGGTATCAACGCAGAGTACATG	1761	0.14016337270801846	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1616	0.1286223794981021	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1478	0.11763853768452656	No Hit
TATCAACGCAGAGTACATGGAAGCA	1400	0.11143027926815777	No Hit
GTACATGGGGTGGTATCAACGCAAA	1363	0.1084853361732136	No Hit
GTATCAACGCAGAGTACATGGGAAG	1355	0.1078485917202527	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1351	0.10753021949377224	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1340	0.106654695870951	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1332	0.1060179514179901	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1306	0.10394853194586717	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1274	0.10140155413402357	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.5918611324022537E-4
13	0.0	0.0	0.0	0.0	1.5918611324022537E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAG	90	2.3046596E-9	14.769349	1
GTCTAGT	60	2.5813304E-5	14.241875	1
AACGGAC	65	5.447422E-5	13.15473	15
GGACATA	60	4.1107836E-4	12.659444	1
GCGTTAT	105	2.0079824E-8	12.659443	1
CGTCTTA	100	1.440294E-7	12.350829	15
GGTTCTA	285	0.0	12.33367	13
AGGCCCG	255	0.0	12.294943	10
ATCGCCA	240	0.0	12.272636	16
CGCATCG	240	0.0	12.271169	13
CGACCAT	155	1.8189894E-12	12.258887	10
CGAACGA	70	1.0890828E-4	12.21608	16
CGCCCGA	55	0.0030645067	12.092685	16
GCGAGAA	55	0.0030654217	12.092202	9
GCATCGC	260	0.0	12.057541	14
CGGTCCA	245	0.0	12.021215	10
AACCGAC	80	2.8598573E-5	11.877217	17
AACGCCG	240	0.0	11.874377	5
TAGGGTC	120	1.0017175E-8	11.873904	4
ATTTGCG	145	9.640644E-11	11.794835	16
>>END_MODULE