FastQCFastQC Report
Thu 2 Feb 2017
SRR4062541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256391
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA54990.4376822183539996No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC47030.37432614528439No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA21690.1726373398090244No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC21360.1700107689405607No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14200.11302214040056001No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13910.11071394175857674No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG13250.10546080002164931No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13120.10442609028558783No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12820.10203829858698447No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG12700.10108318190754312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA401.8202194E-532.9997644
CGATATG904.082358E-721.99984238
GTCGATA803.6159581E-621.9998436
GGCGAAC550.00448389719.99985521
TCGATAT901.0020733E-519.55541437
GATATAC1759.094947E-1218.862261
GTCGTAT1051.7929269E-618.85700643
CTATGCG1658.185452E-1118.66653338
CGATGAT1658.185452E-1118.66653343
TATGGCG2950.018.64393216
GTAGGAC4850.018.5978033
TGTAGGA5100.018.5496232
TGCGCTA600.007412570518.333221
GCGAGAA2650.018.26401920
GTATTAG3750.018.1916031
TAGGACG2600.017.7691024
GCGAAAG5000.017.59987318
CAGAGCG5100.017.25477614
GAGCGAA5300.017.01874416
AGCGAAA5200.016.92295517
Produced by FastQC (version 0.11.5)