##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062541_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1256391
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251038092440968	32.0	32.0	32.0	32.0	32.0
2	31.442839848422985	32.0	32.0	32.0	32.0	32.0
3	31.525798099476994	32.0	32.0	32.0	32.0	32.0
4	31.62924201144389	32.0	32.0	32.0	32.0	32.0
5	31.568561061007284	32.0	32.0	32.0	32.0	32.0
6	35.164674850424746	36.0	36.0	36.0	36.0	36.0
7	35.17700938640917	36.0	36.0	36.0	36.0	36.0
8	35.12733058418916	36.0	36.0	36.0	36.0	36.0
9	35.23471116873648	36.0	36.0	36.0	36.0	36.0
10	35.13152434234247	36.0	36.0	36.0	36.0	36.0
11	35.24466905605023	36.0	36.0	36.0	36.0	36.0
12	35.1623634680605	36.0	36.0	36.0	36.0	36.0
13	35.19359817126993	36.0	36.0	36.0	36.0	36.0
14	35.14898785489549	36.0	36.0	36.0	36.0	36.0
15	35.12173519230876	36.0	36.0	36.0	36.0	36.0
16	35.130988681071415	36.0	36.0	36.0	36.0	36.0
17	35.10018537222887	36.0	36.0	36.0	36.0	36.0
18	35.11486153593905	36.0	36.0	36.0	36.0	36.0
19	35.08385446887155	36.0	36.0	36.0	36.0	36.0
20	35.069043792895684	36.0	36.0	36.0	36.0	36.0
21	35.045482656275	36.0	36.0	36.0	36.0	36.0
22	35.017411777066215	36.0	36.0	36.0	36.0	36.0
23	34.96701982105889	36.0	36.0	36.0	36.0	36.0
24	34.942752694025984	36.0	36.0	36.0	32.0	36.0
25	34.912332227785775	36.0	36.0	36.0	32.0	36.0
26	34.86251732143894	36.0	36.0	36.0	32.0	36.0
27	34.82735868053815	36.0	36.0	36.0	32.0	36.0
28	34.80232984795338	36.0	36.0	36.0	32.0	36.0
29	34.76429391805576	36.0	36.0	36.0	32.0	36.0
30	34.74473472032194	36.0	36.0	36.0	32.0	36.0
31	34.73196401438724	36.0	36.0	36.0	32.0	36.0
32	34.68810824018956	36.0	36.0	36.0	32.0	36.0
33	34.6527577800223	36.0	36.0	36.0	32.0	36.0
34	34.618295578366926	36.0	36.0	36.0	32.0	36.0
35	34.54365639359085	36.0	36.0	36.0	32.0	36.0
36	34.51620395243201	36.0	36.0	36.0	32.0	36.0
37	34.46322999766792	36.0	36.0	36.0	32.0	36.0
38	34.404939226721616	36.0	36.0	36.0	32.0	36.0
39	34.306200060331534	36.0	36.0	36.0	32.0	36.0
40	34.23660627941461	36.0	36.0	36.0	32.0	36.0
41	34.23504307178259	36.0	36.0	36.0	32.0	36.0
42	34.1143704467797	36.0	36.0	36.0	32.0	36.0
43	34.12581752018281	36.0	36.0	36.0	32.0	36.0
44	34.09873916639008	36.0	36.0	36.0	32.0	36.0
45	33.948273268433155	36.0	36.0	36.0	32.0	36.0
46	34.03604689941268	36.0	36.0	36.0	32.0	36.0
47	33.93779325066799	36.0	36.0	36.0	32.0	36.0
48	33.926466362780374	36.0	36.0	36.0	32.0	36.0
49	33.94566977955111	36.0	36.0	36.0	32.0	36.0
50	33.384532362934785	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	13.0
21	14.0
22	134.0
23	345.0
24	993.0
25	2492.0
26	5200.0
27	10064.0
28	16862.0
29	24746.0
30	34213.0
31	46916.0
32	67994.0
33	98061.0
34	205196.0
35	743147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.885571656128285	17.512802489410532	11.866413097903918	25.735212756557267
2	15.67640452300105	20.72824583271646	36.36007622176905	27.23527342251344
3	18.94037679382994	24.490086676907648	28.42525012137951	28.144286407882902
4	12.410656580610448	15.719745811007426	35.19302954182943	36.67656806655269
5	14.096407885761678	36.86742423337957	33.407912027386374	15.628255853472368
6	34.88362652878972	34.419915535512175	16.715006375429205	13.981451560268898
7	29.815423554787923	30.729072978931697	20.863028199842407	18.59247526643797
8	28.826456095276072	32.29185818745916	19.802991266253898	19.078694451010872
9	27.22695402943829	14.384057192386765	18.380344972226005	40.00864380594894
10	15.702277396129071	27.09451118322242	31.810956939360434	25.392254481288067
11	37.50416868634048	21.25214204813629	21.153048692644248	20.090640572878986
12	24.00502709745613	24.076740441470847	28.889732575289063	23.028499885783965
13	30.40884565394053	19.309593908265818	25.063933122730102	25.217627315063545
14	23.957191670427438	19.388231848206487	24.965635697804267	31.688940783561804
15	25.808446574354637	26.442882828673557	23.55015277887218	24.19851781809962
16	26.23307553142294	25.41223233849972	24.358181489679566	23.996510640397776
17	24.073556719206042	25.71826764120405	26.114561470115593	24.09361416947431
18	25.51976255799349	23.771421476276096	27.486745766246333	23.222070199484076
19	25.000099491320775	26.457050392751935	25.480761960249637	23.06208815567765
20	24.89790200662055	23.629507056322435	27.22846629751407	24.244124639542942
21	26.044201208063413	24.949637493423623	24.166839781564818	24.83932151694815
22	24.603009731843034	25.17170212139374	25.221925340121032	25.003362806642198
23	23.631258103568076	24.46642804668292	25.50647051753793	26.395843332211072
24	23.78248491114629	26.078426222410062	25.436587813825472	24.70250105261817
25	25.20139033151304	24.24579609373197	24.92623713477731	25.626576439977683
26	23.632531592474	24.962451975539462	26.107955246416125	25.297061185570413
27	25.660005523758127	25.23107854163234	25.29172845077687	23.81718748383266
28	24.383014523345043	23.895586644603473	26.09673262543269	25.6246662066188
29	23.89311925984825	25.392015702118208	26.58479724862722	24.130067789406322
30	25.083910979941752	24.568386752213282	26.432694917426186	23.91500735041878
31	23.988790113905623	24.96746633810653	25.36598877260343	25.677754775384415
32	23.938805674348192	25.30923892323329	25.505276621688633	25.246678780729887
33	23.681242543125506	24.5941749025582	26.058527958255034	25.66605459606126
34	23.982502262432632	25.3983035535912	26.640432795204678	23.97876138877149
35	25.73267398445229	24.74142205730541	25.75806416951411	23.76783978872819
36	24.237518415843475	25.721292177355615	25.596251485405418	24.44493792139549
37	25.531223958146786	25.21062312608097	25.589963633932427	23.668189281839812
38	24.365742830058476	25.148301762747426	25.204096495438122	25.28185891175597
39	24.451384958981716	24.9056225331127	25.729171889961005	24.913820617944573
40	25.32802288459564	25.06552498386251	25.183322707660277	24.42312942388158
41	23.430046856432433	25.328978001275082	26.43150102157688	24.809474120715606
42	25.94192413030657	25.50615214531145	25.431175485975306	23.120748238406673
43	24.797853534449068	23.90219286830294	25.334629108295108	25.965324488952884
44	24.253118654941016	24.857787106084015	26.421233517272885	24.46786072170208
45	24.73449746137946	25.023022291627367	25.617662017636228	24.624818229356944
46	23.85300435931171	24.758375378365493	26.504487854497526	24.88413240782527
47	24.829451977927253	24.210615962705877	26.761732613493727	24.19819944587314
48	24.669469934120826	26.802723037653088	24.56631733274116	23.961489695484925
49	23.566389762422684	25.055814630954853	26.806623097427472	24.571172509194987
50	23.58431697164097	26.835695922444465	24.42171618685281	25.158270919061753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	18.5
2	25.0
3	80.5
4	136.0
5	155.0
6	174.0
7	201.5
8	229.0
9	308.0
10	387.0
11	613.5
12	840.0
13	1480.0
14	2120.0
15	3102.0
16	4084.0
17	4855.0
18	5626.0
19	6128.5
20	6631.0
21	7128.5
22	7626.0
23	7944.5
24	8263.0
25	8473.0
26	8683.0
27	10498.0
28	12313.0
29	14024.5
30	15736.0
31	17413.5
32	19091.0
33	22007.5
34	24924.0
35	28438.0
36	31952.0
37	38147.0
38	44342.0
39	47557.5
40	50773.0
41	56213.0
42	61653.0
43	65785.0
44	69917.0
45	81851.5
46	93786.0
47	103678.5
48	113571.0
49	110348.5
50	107126.0
51	97429.0
52	87732.0
53	84274.5
54	80817.0
55	81976.0
56	83135.0
57	80341.0
58	77547.0
59	70606.0
60	63665.0
61	55886.0
62	48107.0
63	42297.0
64	36487.0
65	31123.0
66	25759.0
67	22464.5
68	19170.0
69	17478.5
70	15787.0
71	12464.0
72	9141.0
73	8311.5
74	7482.0
75	5539.5
76	3597.0
77	3201.0
78	2805.0
79	2421.0
80	2037.0
81	1646.5
82	1256.0
83	1074.0
84	892.0
85	732.5
86	573.0
87	420.0
88	267.0
89	170.0
90	73.0
91	44.5
92	16.0
93	12.5
94	9.0
95	9.5
96	10.0
97	6.5
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015122680757821413
2	0.004536804227346423
3	7.959305662011269E-5
4	2.3877916986033806E-4
5	0.0
6	3.979652831005634E-4
7	7.959305662011269E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	7.959305662011269E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1256391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.448975597030625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12180204060347	45.22793648180144
2	12.597467069017021	14.222282223266777
3	3.390917042280136	5.7424138011368004
4	1.3802648379049087	3.116581446093346
5	0.6883919623811976	1.9429510542824122
6	0.402969922153719	1.364834358119557
7	0.2638677085185742	1.0426543287306573
8	0.17081554357591672	0.7713889960728356
9	0.13464020942238308	0.6840271706456877
>10	0.6780858887650473	7.15520757953122
>50	0.07640748895037426	3.0738065131584955
>100	0.08287556898015616	9.659922578453592
>500	0.008514605516417605	3.2006669210713894
>1k	0.002838201838805868	2.3548192919988624
>5k	1.419100919402934E-4	0.4405072556368807
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5499	0.4376822183539996	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4703	0.37432614528439	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2169	0.1726373398090244	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2136	0.1700107689405607	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1420	0.11302214040056001	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1391	0.11071394175857674	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1325	0.10546080002164931	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1312	0.10442609028558783	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1282	0.10203829858698447	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1270	0.10108318190754312	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.959305662011269E-5
2	0.0	0.0	0.0	0.0	7.959305662011269E-5
3	0.0	0.0	0.0	0.0	7.959305662011269E-5
4	0.0	0.0	0.0	0.0	7.959305662011269E-5
5	0.0	0.0	0.0	0.0	7.959305662011269E-5
6	0.0	0.0	0.0	0.0	7.959305662011269E-5
7	0.0	0.0	0.0	0.0	7.959305662011269E-5
8	0.0	0.0	0.0	0.0	7.959305662011269E-5
9	0.0	0.0	0.0	0.0	7.959305662011269E-5
10	0.0	0.0	0.0	0.0	7.959305662011269E-5
11	0.0	0.0	0.0	0.0	7.959305662011269E-5
12	0.0	0.0	0.0	0.0	2.3877916986033806E-4
13	0.0	0.0	0.0	0.0	2.3877916986033806E-4
14	0.0	0.0	0.0	0.0	2.3877916986033806E-4
15	0.0	0.0	0.0	0.0	3.1837222648045075E-4
16	0.0	0.0	0.0	0.0	3.1837222648045075E-4
17	0.0	0.0	0.0	0.0	3.1837222648045075E-4
18	0.0	0.0	0.0	0.0	3.1837222648045075E-4
19	0.0	0.0	0.0	0.0	3.1837222648045075E-4
20	0.0	7.959305662011269E-5	0.0	1.5918611324022537E-4	3.9796528310056346E-4
21	0.0	7.959305662011269E-5	0.0	4.775583397206761E-4	3.9796528310056346E-4
22	0.0	7.959305662011269E-5	0.0	0.0015918611324022538	3.9796528310056346E-4
23	7.959305662011269E-5	7.959305662011269E-5	0.0	0.0028653500383240566	3.9796528310056346E-4
24	7.959305662011269E-5	7.959305662011269E-5	0.0	0.004536804227346423	3.9796528310056346E-4
25	7.959305662011269E-5	7.959305662011269E-5	0.0	0.00620825841636879	3.9796528310056346E-4
26	7.959305662011269E-5	7.959305662011269E-5	0.0	0.008516457058352057	3.9796528310056346E-4
27	7.959305662011269E-5	7.959305662011269E-5	0.0	0.011063434870195664	3.9796528310056346E-4
28	7.959305662011269E-5	7.959305662011269E-5	0.0	0.01719210022994434	3.9796528310056346E-4
29	7.959305662011269E-5	7.959305662011269E-5	0.0	0.030245361515642822	3.9796528310056346E-4
30	7.959305662011269E-5	7.959305662011269E-5	0.0	0.05388449933181629	3.9796528310056346E-4
31	7.959305662011269E-5	7.959305662011269E-5	0.0	0.10283422915318559	3.9796528310056346E-4
32	7.959305662011269E-5	7.959305662011269E-5	0.0	0.16420047580729247	3.9796528310056346E-4
33	7.959305662011269E-5	7.959305662011269E-5	0.0	0.21808497513910877	3.9796528310056346E-4
34	7.959305662011269E-5	7.959305662011269E-5	0.0	0.2937779719848359	4.775583397206761E-4
35	7.959305662011269E-5	7.959305662011269E-5	0.0	0.3647749784899765	4.775583397206761E-4
36	7.959305662011269E-5	7.959305662011269E-5	0.0	0.4801053175325197	4.775583397206761E-4
37	7.959305662011269E-5	7.959305662011269E-5	0.0	0.6581549851917118	4.775583397206761E-4
38	7.959305662011269E-5	7.959305662011269E-5	0.0	0.8667683865930271	4.775583397206761E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	40	1.8202194E-5	32.99976	44
CGATATG	90	4.082358E-7	21.999842	38
GTCGATA	80	3.6159581E-6	21.99984	36
GGCGAAC	55	0.004483897	19.999855	21
TCGATAT	90	1.0020733E-5	19.555414	37
GATATAC	175	9.094947E-12	18.86226	1
GTCGTAT	105	1.7929269E-6	18.857006	43
CTATGCG	165	8.185452E-11	18.666533	38
CGATGAT	165	8.185452E-11	18.666533	43
TATGGCG	295	0.0	18.643932	16
GTAGGAC	485	0.0	18.597803	3
TGTAGGA	510	0.0	18.549623	2
TGCGCTA	60	0.0074125705	18.3332	21
GCGAGAA	265	0.0	18.264019	20
GTATTAG	375	0.0	18.191603	1
TAGGACG	260	0.0	17.769102	4
GCGAAAG	500	0.0	17.599873	18
CAGAGCG	510	0.0	17.254776	14
GAGCGAA	530	0.0	17.018744	16
AGCGAAA	520	0.0	16.922955	17
>>END_MODULE