FastQCFastQC Report
Thu 2 Feb 2017
SRR4062540_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062540_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174662
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66080.5625448001212263No Hit
GTACATGGAAGCAGTGGTATCAACG55940.4762220962285321No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52930.45059770385012876No Hit
TCCATGTACTCTGCGTTGATACCAC46490.3957734224823822No Hit
TATCAACGCAGAGTACTTTTTTTTT46160.3929641037166436No Hit
GAGTACTTTTTTTTTTTTTTTTTTT36310.30911019510293175No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27850.2370894776539975No Hit
GTACATGGGGTGGTATCAACGCAAA27640.2353017293485275No Hit
GAGTACATGGAAGCAGTGGTATCAA23680.20158990415966466No Hit
GGTATCAACGCAGAGTACTTTTTTT23230.19775901493365752No Hit
CATGTACTCTGCGTTGATACCACTG21170.1802220553657137No Hit
GTACATGGGTGGTATCAACGCAAAA16240.13825253562301326No Hit
CCCATGTACTCTGCGTTGATACCAC15710.13374059942349373No Hit
GCTTCCATGTACTCTGCGTTGATAC15360.13076101891437708No Hit
GTACATGGGAAGCAGTGGTATCAAC14540.12378028743587517No Hit
GCGTTGATACCACTGCTTCCATGTA13090.11143631104096327No Hit
GCAGAGTACTTTTTTTTTTTTTTTT12980.11049987145238375No Hit
GTACATGGGAGTGGTATCAACGCAA12910.10990395535056043No Hit
GTATCAACGCAGAGTACATGGGGTG12750.10854186140353565No Hit
CATGGAAGCAGTGGTATCAACGCAG12730.10837159966015757No Hit
GTATCAACGCAGAGTACATGGGAAG12730.10837159966015757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCG400.00527266114.2522079
CGCGCTA655.4379947E-513.15700616
TCGGCGT1102.737579E-912.95599913
GAGCGAA1850.012.84092116
ATCGCCA1207.366907E-1012.66970916
CGAAAGC1950.012.66808819
GTCGGCG1051.9883373E-812.66808812
CGCATCG1207.385097E-1012.66808713
CTATACC905.407783E-712.6653874
CGCAAGA1850.012.3173042
GCGTTAT701.09889654E-412.2042071
AGAATTT2350.012.13005417
GCGAAAG1900.012.00236918
AGCGAAA1900.012.00236917
TTAATCC951.0403073E-611.9987894
CGGTTCT1701.8189894E-1211.73661112
CAAGACG1950.011.6911284
ATTTACC658.0420094E-411.6886363
CAGAGCG2300.011.56799414
GAATTTC2300.011.56750118
Produced by FastQC (version 0.11.5)