##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062540_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174662
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90094256901134	32.0	32.0	32.0	32.0	32.0
2	30.658143363793158	32.0	32.0	32.0	32.0	32.0
3	30.685054083642783	32.0	32.0	32.0	32.0	32.0
4	30.689472375883447	32.0	32.0	32.0	32.0	32.0
5	30.571533768862874	32.0	32.0	32.0	32.0	32.0
6	34.14946767665933	36.0	36.0	36.0	32.0	36.0
7	33.948481350379936	36.0	36.0	36.0	32.0	36.0
8	33.91520113871054	36.0	36.0	36.0	32.0	36.0
9	34.01954179159622	36.0	36.0	36.0	32.0	36.0
10	33.77305386570775	36.0	36.0	36.0	32.0	36.0
11	34.135295089140534	36.0	36.0	36.0	32.0	36.0
12	33.9146043713	36.0	36.0	36.0	32.0	36.0
13	34.02592405304675	36.0	36.0	36.0	32.0	36.0
14	33.94594104516874	36.0	36.0	36.0	32.0	36.0
15	33.934182769171045	36.0	36.0	36.0	32.0	36.0
16	33.90506545712724	36.0	36.0	36.0	32.0	36.0
17	33.832509266495386	36.0	36.0	36.0	32.0	36.0
18	33.87668027057996	36.0	36.0	36.0	32.0	36.0
19	33.838041070537734	36.0	36.0	36.0	32.0	36.0
20	33.76513073547965	36.0	36.0	36.0	32.0	36.0
21	33.724932789176805	36.0	36.0	36.0	27.0	36.0
22	33.69312619289634	36.0	36.0	36.0	27.0	36.0
23	33.69895765760704	36.0	36.0	36.0	27.0	36.0
24	33.701652901004714	36.0	36.0	36.0	27.0	36.0
25	33.219725333755584	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	10.0
4	50.0
5	100.0
6	222.0
7	63.0
8	185.0
9	204.0
10	145.0
11	44.0
12	106.0
13	65.0
14	417.0
15	473.0
16	624.0
17	907.0
18	1382.0
19	1843.0
20	2724.0
21	3951.0
22	5492.0
23	7656.0
24	10022.0
25	12836.0
26	16539.0
27	20587.0
28	26839.0
29	34349.0
30	43430.0
31	57332.0
32	80798.0
33	115707.0
34	253450.0
35	476108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.54275086606826	17.382749810354678	11.37335566224317	24.701143661333894
2	15.888875267323227	20.75007151321973	37.909923991663604	25.451129227793444
3	19.23025514282771	25.629726621130484	28.16753320500563	26.972485031036175
4	12.265992781504712	15.84152346181256	35.717247503795285	36.17523625288744
5	13.893494732306111	37.628903292617395	33.19375285824875	15.28384911682775
6	32.945034260430376	35.13293217329092	17.929809768374792	13.992223797903918
7	29.265872001690198	31.404430632085788	21.14841356655319	18.18128379967082
8	28.482166213497596	32.2611787622051	20.082448084377212	19.17420693992009
9	27.72036510426742	14.565482120168083	19.131330673990796	38.582822101573704
10	15.918935828175051	27.596203185491913	31.792099175413448	24.692761810919592
11	36.06484855458811	22.052429476770698	22.207987589445917	19.674734379195275
12	24.425811093512102	24.70192984759336	29.288621247589607	21.58363781130493
13	29.826577179377466	20.32292819139415	25.59073373553744	24.25976089369094
14	23.572568354080786	20.078574375445378	26.41814046895724	29.930716801516592
15	24.836376250670135	27.16149977285725	24.453855929278063	23.548268047194554
16	24.458977215293444	26.645057858818856	25.202103151232393	23.693861774655307
17	22.820426926201247	26.368229175989644	26.51526581240512	24.296078085403995
18	23.864162369563125	25.28734260410868	28.061329307453814	22.78716571887438
19	24.23179184493563	26.45447439107244	26.731094965676032	22.582638798315894
20	24.801009231398687	24.457069307943435	27.346505621521178	23.3954158391367
21	25.8458957475901	24.872472030797663	25.520767622899115	23.760864598713127
22	24.608339173926908	25.432843405586166	26.054333720432528	23.9044837000544
23	23.20328944732272	25.501145389922787	26.502626255048114	24.79293890770638
24	23.25489009279348	26.596648863844642	26.596819361741126	23.55164168162075
25	23.91188523017985	25.64870880542444	26.33290169905913	24.106504265336586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	580.0
1	580.0
2	1152.5
3	1725.0
4	1725.0
5	1725.0
6	4438.5
7	7152.0
8	7152.0
9	7152.0
10	6822.5
11	6493.0
12	6493.0
13	6493.0
14	5649.0
15	4805.0
16	4805.0
17	4805.0
18	7542.0
19	10279.0
20	10279.0
21	10279.0
22	15802.0
23	21325.0
24	21325.0
25	21325.0
26	30697.0
27	40069.0
28	40069.0
29	40069.0
30	48850.0
31	57631.0
32	57631.0
33	57631.0
34	67594.0
35	77557.0
36	77557.0
37	77557.0
38	86345.0
39	95133.0
40	95133.0
41	95133.0
42	109094.5
43	123056.0
44	123056.0
45	123056.0
46	148514.5
47	173973.0
48	173973.0
49	173973.0
50	166240.5
51	158508.0
52	158508.0
53	158508.0
54	140701.0
55	122894.0
56	122894.0
57	122894.0
58	110974.5
59	99055.0
60	99055.0
61	99055.0
62	87013.0
63	74971.0
64	74971.0
65	74971.0
66	61195.0
67	47419.0
68	47419.0
69	47419.0
70	36081.0
71	24743.0
72	24743.0
73	24743.0
74	18964.5
75	13186.0
76	13186.0
77	13186.0
78	10772.5
79	8359.0
80	8359.0
81	8359.0
82	5736.5
83	3114.0
84	3114.0
85	3114.0
86	2264.5
87	1415.0
88	1415.0
89	1415.0
90	966.0
91	517.0
92	517.0
93	517.0
94	316.0
95	115.0
96	115.0
97	115.0
98	351.5
99	588.0
100	588.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008598218040593806
2	0.004597067071208569
3	0.005533506659788092
4	0.015238426032339514
5	0.03652114395460141
6	0.04826920424768997
7	0.07099914698866568
8	0.09151568706572615
9	0.10122060643827757
10	0.12497211963952183
11	0.12488698876783279
12	0.1375714886495009
13	0.12429107266600946
14	0.12820709276370565
15	0.11841704251946517
16	0.11705494857244042
17	0.12641934445823563
18	0.13493243162714041
19	0.13408112291024993
20	0.12701526056005896
21	0.13501756249882946
22	0.15630028042109134
23	0.14463735099969183
24	0.13850792823808042
25	0.13629452557416516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1174662.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.313194643210224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3553193794963	41.47101323503064
2	14.650327390036555	15.91412166243619
3	4.565222300588631	7.438554222041831
4	1.806109095497252	3.9238221940245843
5	0.8365534088236495	2.271794406143995
6	0.44102969879435416	1.4372239124432484
7	0.2633995584426097	1.0014250040640362
8	0.1664554223617091	0.7232580603319421
9	0.12127896068440444	0.5928343018000468
>10	0.629263287841554	6.559457395041599
>50	0.07876790769739926	3.0503430784988845
>100	0.07637334519559948	8.32304489760049
>500	0.006443016287995366	2.3664533068582667
>1k	0.0029857880359002915	3.4334313343066976
>5k	4.714402161947829E-4	1.4932229893776734
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6608	0.5625448001212263	No Hit
GTACATGGAAGCAGTGGTATCAACG	5594	0.4762220962285321	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5293	0.45059770385012876	No Hit
TCCATGTACTCTGCGTTGATACCAC	4649	0.3957734224823822	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4616	0.3929641037166436	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3631	0.30911019510293175	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2785	0.2370894776539975	No Hit
GTACATGGGGTGGTATCAACGCAAA	2764	0.2353017293485275	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2368	0.20158990415966466	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2323	0.19775901493365752	No Hit
CATGTACTCTGCGTTGATACCACTG	2117	0.1802220553657137	No Hit
GTACATGGGTGGTATCAACGCAAAA	1624	0.13825253562301326	No Hit
CCCATGTACTCTGCGTTGATACCAC	1571	0.13374059942349373	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1536	0.13076101891437708	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1454	0.12378028743587517	No Hit
GCGTTGATACCACTGCTTCCATGTA	1309	0.11143631104096327	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1298	0.11049987145238375	No Hit
GTACATGGGAGTGGTATCAACGCAA	1291	0.10990395535056043	No Hit
GTATCAACGCAGAGTACATGGGGTG	1275	0.10854186140353565	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1273	0.10837159966015757	No Hit
GTATCAACGCAGAGTACATGGGAAG	1273	0.10837159966015757	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.513087168904757E-5	0.0
5	0.0	0.0	0.0	8.513087168904757E-5	0.0
6	0.0	0.0	0.0	8.513087168904757E-5	0.0
7	0.0	0.0	0.0	1.7026174337809514E-4	0.0
8	0.0	0.0	0.0	1.7026174337809514E-4	0.0
9	0.0	0.0	0.0	2.5539261506714274E-4	0.0
10	0.0	0.0	0.0	5.107852301342855E-4	0.0
11	0.0	0.0	0.0	5.107852301342855E-4	0.0
12	0.0	0.0	0.0	5.107852301342855E-4	8.513087168904757E-5
13	0.0	0.0	0.0	5.107852301342855E-4	8.513087168904757E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGCG	40	0.005272661	14.252207	9
CGCGCTA	65	5.4379947E-5	13.157006	16
TCGGCGT	110	2.737579E-9	12.955999	13
GAGCGAA	185	0.0	12.840921	16
ATCGCCA	120	7.366907E-10	12.669709	16
CGAAAGC	195	0.0	12.668088	19
GTCGGCG	105	1.9883373E-8	12.668088	12
CGCATCG	120	7.385097E-10	12.668087	13
CTATACC	90	5.407783E-7	12.665387	4
CGCAAGA	185	0.0	12.317304	2
GCGTTAT	70	1.09889654E-4	12.204207	1
AGAATTT	235	0.0	12.130054	17
GCGAAAG	190	0.0	12.002369	18
AGCGAAA	190	0.0	12.002369	17
TTAATCC	95	1.0403073E-6	11.998789	4
CGGTTCT	170	1.8189894E-12	11.736611	12
CAAGACG	195	0.0	11.691128	4
ATTTACC	65	8.0420094E-4	11.688636	3
CAGAGCG	230	0.0	11.567994	14
GAATTTC	230	0.0	11.567501	18
>>END_MODULE