FastQCFastQC Report
Thu 2 Feb 2017
SRR4062540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174662
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24650.20984759871350228No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC22300.1898418438665761No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20250.17239001517032135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.1444670892563137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCG508.34145E-526.3998998
TATCTCG500.002580452521.99991627
TATACCG500.002580452521.9999165
GTATTAT956.854989E-720.8455731
GTGATCG755.4726283E-520.5332559
TTCGGTC600.007412145418.33326312
TAATACT2800.018.071364
TATCGTT750.001290874217.59993211
GTGTATA2251.8189894E-1216.624991
TACCGTC2400.016.4999377
TACGCTA2550.016.3920949
GATTTCG1259.440077E-615.83993941
ACGCTAT2650.015.77352310
AGTGCGG850.002979951215.52935110
CGAGCCG3550.015.49289815
ATACCGT2700.015.4814216
GATCGAA1004.9436896E-415.39994111
TAGGGTG2001.2751116E-915.3999415
TATAAGC1004.9436896E-415.3999413
CTAATAC3750.015.2532743
Produced by FastQC (version 0.11.5)