##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062540_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1174662
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08613456466626	32.0	32.0	32.0	32.0	32.0
2	31.389073622880453	32.0	32.0	32.0	32.0	32.0
3	31.48717588548876	32.0	32.0	32.0	32.0	32.0
4	31.605993043105165	32.0	32.0	32.0	32.0	32.0
5	31.545637809003782	32.0	32.0	32.0	32.0	32.0
6	35.11785773269247	36.0	36.0	36.0	36.0	36.0
7	35.12212108674665	36.0	36.0	36.0	36.0	36.0
8	35.081847373968	36.0	36.0	36.0	36.0	36.0
9	35.161608190270904	36.0	36.0	36.0	36.0	36.0
10	35.075328903122774	36.0	36.0	36.0	36.0	36.0
11	35.197839037953045	36.0	36.0	36.0	36.0	36.0
12	35.10418911993408	36.0	36.0	36.0	36.0	36.0
13	35.12440770196022	36.0	36.0	36.0	36.0	36.0
14	35.09015699835357	36.0	36.0	36.0	36.0	36.0
15	35.075833729191885	36.0	36.0	36.0	36.0	36.0
16	35.08007494921944	36.0	36.0	36.0	36.0	36.0
17	35.06375791504279	36.0	36.0	36.0	36.0	36.0
18	35.05876584072695	36.0	36.0	36.0	36.0	36.0
19	35.02887043251591	36.0	36.0	36.0	36.0	36.0
20	34.98650675683729	36.0	36.0	36.0	36.0	36.0
21	34.95971947675161	36.0	36.0	36.0	36.0	36.0
22	34.96889062555867	36.0	36.0	36.0	36.0	36.0
23	34.93233117271181	36.0	36.0	36.0	32.0	36.0
24	34.92992792820403	36.0	36.0	36.0	32.0	36.0
25	34.8972206473011	36.0	36.0	36.0	32.0	36.0
26	34.85086263112282	36.0	36.0	36.0	32.0	36.0
27	34.83788187580768	36.0	36.0	36.0	32.0	36.0
28	34.80630257895463	36.0	36.0	36.0	32.0	36.0
29	34.77662170054024	36.0	36.0	36.0	32.0	36.0
30	34.75647037190273	36.0	36.0	36.0	32.0	36.0
31	34.738981085622925	36.0	36.0	36.0	32.0	36.0
32	34.68414999378545	36.0	36.0	36.0	32.0	36.0
33	34.62093095716044	36.0	36.0	36.0	32.0	36.0
34	34.58281956852269	36.0	36.0	36.0	32.0	36.0
35	34.52217999730986	36.0	36.0	36.0	32.0	36.0
36	34.48192331070555	36.0	36.0	36.0	32.0	36.0
37	34.44587464308882	36.0	36.0	36.0	32.0	36.0
38	34.39697802431678	36.0	36.0	36.0	32.0	36.0
39	34.33641847612334	36.0	36.0	36.0	32.0	36.0
40	34.28107659905573	36.0	36.0	36.0	32.0	36.0
41	34.2410463605701	36.0	36.0	36.0	32.0	36.0
42	34.16359429350741	36.0	36.0	36.0	32.0	36.0
43	34.13669549197982	36.0	36.0	36.0	32.0	36.0
44	34.08232240423202	36.0	36.0	36.0	32.0	36.0
45	33.964197360602455	36.0	36.0	36.0	32.0	36.0
46	33.96801888543258	36.0	36.0	36.0	32.0	36.0
47	33.864917738038685	36.0	36.0	36.0	32.0	36.0
48	33.814113336432094	36.0	36.0	36.0	32.0	36.0
49	33.770934107002695	36.0	36.0	36.0	27.0	36.0
50	33.2357486664249	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	3.0
21	45.0
22	150.0
23	427.0
24	1102.0
25	2752.0
26	5540.0
27	10382.0
28	17038.0
29	24694.0
30	33468.0
31	44682.0
32	62849.0
33	92515.0
34	188411.0
35	690600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.748569959180614	18.110728328857448	11.966533894394104	25.174167817567835
2	15.39374435987809	21.159949600721937	37.34501370656042	26.101292332839556
3	18.648384971234083	25.713784654885607	28.75817049105524	26.87965988282507
4	12.100842625367978	16.101312547779703	35.81200379343164	35.98584103342068
5	13.587312775930439	37.68360600751535	33.35751050089302	15.371570715661187
6	33.093802621699254	35.373189473021085	17.53114523546428	14.001862669815385
7	29.027498974172993	31.35020967733697	21.29719017044903	18.325101178041002
8	28.397275131058976	32.163039240223995	20.228116683777973	19.211568944939057
9	27.664213194944587	14.154369512251183	19.223146743488766	38.95827054931546
10	15.839194593849124	27.44772538823934	32.128561237189935	24.584518780721602
11	36.274690081061614	21.684109982275753	22.301223671149657	19.739976265512972
12	24.238376656433935	24.592350820916995	29.563312680583863	21.605959842065207
13	29.976367670019123	20.062026353112643	25.79559056137	24.166015415498247
14	23.58814705847299	19.74389228561067	26.641110378985616	30.026850276930727
15	24.876773063230104	27.020794066718768	24.574898992220742	23.52753387783039
16	24.578304227088303	26.136709964228004	25.405010122060645	23.879975686623045
17	22.900204484353797	25.968065707412002	26.902206762455922	24.229523045778276
18	23.92986237743283	24.637385052040504	28.567962528795515	22.864790041731155
19	24.316016011414348	25.916987184398575	27.12908053550723	22.63791626867984
20	24.757845235480506	23.942376615571117	28.007716262209897	23.292061886738484
21	25.70118042466684	24.48653314740751	26.21962743325314	23.59265899467251
22	24.513775026348007	24.849956838648055	26.69006063020682	23.946207504797123
23	23.139933019030153	24.847402912497383	27.235323863375164	24.777340205097296
24	23.387578724773594	25.93988738888293	27.150874038659634	23.521659847683846
25	24.09944307383741	24.803986167935967	26.92834194006446	24.16822881816216
26	23.199269236597424	25.417268967583865	27.597470591540375	23.785991204278336
27	24.297457481386132	25.65137886472875	26.919828852895556	23.13133480098956
28	23.273247964095205	25.00276675332989	27.61373058803298	24.11025469454192
29	23.037265187773166	25.756089836906277	27.851841636147253	23.354803339173312
30	23.71073551370522	25.49278005077205	27.62147749735669	23.175006938166042
31	23.29223214848186	25.601492173918967	26.8176718068687	24.288603870730473
32	23.072764761267496	25.835942594550605	26.884073886786158	24.207218757395744
33	23.052503613805502	25.390367612130127	27.180584712879106	24.37654406118526
34	23.359996322346344	25.683388072483833	27.634502520725114	23.322113084444716
35	24.34521590040369	25.50623072849892	27.02062380497539	23.127929566122
36	23.45491724427963	26.105466934318127	26.792217676233676	23.64739814516857
37	24.25659466297539	25.974195130173616	26.55206348719887	23.21714671965212
38	23.440104472605736	25.963724032955867	26.635747134069206	23.960424360369196
39	23.489565509057073	25.878678292138506	26.73177475733445	23.899981441469972
40	23.996945504323797	25.936652415758747	26.69244429461411	23.373957785303347
41	22.823756961577033	26.154587447282708	27.196078531526513	23.825577059613746
42	24.258978327382685	26.268066899244207	26.64775058697736	22.825204186395744
43	23.360847631063233	25.348398092387427	26.527375534409046	24.763378742140294
44	22.989677030498985	25.87450687942574	27.02326286199775	24.112553228077523
45	23.314110782505946	26.022719727036375	26.588244107666714	24.074925382790966
46	22.877219148997753	25.809892547813757	26.915657440182795	24.3972308630057
47	23.4450420631637	25.48954507764787	27.081662639976432	23.983750219211995
48	23.493055874796323	27.015005167443913	25.689091840886995	23.80284711687277
49	22.7960042974064	26.267896637500833	26.6995101569643	24.236588908128464
50	22.485702270099825	27.424314398524853	25.613069972468676	24.476913358906646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	63.0
2	63.0
3	246.0
4	429.0
5	488.5
6	548.0
7	506.5
8	465.0
9	671.0
10	877.0
11	1251.0
12	1625.0
13	2891.5
14	4158.0
15	5858.5
16	7559.0
17	8712.5
18	9866.0
19	10477.0
20	11088.0
21	11717.0
22	12346.0
23	12480.0
24	12614.0
25	12019.5
26	11425.0
27	12563.0
28	13701.0
29	15910.0
30	18119.0
31	20875.5
32	23632.0
33	26725.5
34	29819.0
35	32847.5
36	35876.0
37	40579.5
38	45283.0
39	48091.5
40	50900.0
41	55992.5
42	61085.0
43	63893.0
44	66701.0
45	74466.0
46	82231.0
47	87256.5
48	92282.0
49	91960.5
50	91639.0
51	85580.5
52	79522.0
53	76474.5
54	73427.0
55	72388.0
56	71349.0
57	68560.0
58	65771.0
59	59963.0
60	54155.0
61	48070.5
62	41986.0
63	36320.5
64	30655.0
65	26574.0
66	22493.0
67	19462.5
68	16432.0
69	14761.0
70	13090.0
71	10178.5
72	7267.0
73	6495.5
74	5724.0
75	4221.0
76	2718.0
77	2436.0
78	2154.0
79	1782.5
80	1411.0
81	1149.0
82	887.0
83	735.5
84	584.0
85	488.0
86	392.0
87	285.5
88	179.0
89	111.5
90	44.0
91	26.5
92	9.0
93	8.5
94	8.0
95	5.5
96	3.0
97	4.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.018728791771590467
2	0.0035754966109399983
3	6.810469735123806E-4
4	0.0
5	0.0
6	3.405234867561903E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1174662.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.10951504461619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.59893852978334	45.868356244724765
2	14.045690189884736	16.6046787118202
3	4.2262933170039405	7.494424452132159
4	1.6639041080878103	3.9341025963926044
5	0.7932675261611155	2.344482938601296
6	0.42481794837630255	1.5066469746463225
7	0.23367396397297882	0.9668648282297126
8	0.16661253730055817	0.7878709024151217
9	0.12455533867471232	0.6626165098752155
>10	0.583235474783499	6.460202547547329
>50	0.06692215712114113	2.793471365537115
>100	0.0658518463654547	7.519409633681678
>500	0.005366774696003417	2.1498589998877224
>1k	8.702877885410946E-4	0.9070132945089617
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2465	0.20984759871350228	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2230	0.1898418438665761	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2025	0.17239001517032135	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1697	0.1444670892563137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.513087168904757E-5	0.0
10	0.0	0.0	0.0	5.107852301342855E-4	0.0
11	0.0	0.0	0.0	5.107852301342855E-4	0.0
12	0.0	0.0	0.0	5.107852301342855E-4	2.5539261506714274E-4
13	0.0	0.0	0.0	5.107852301342855E-4	4.256543584452379E-4
14	0.0	0.0	0.0	5.95916101823333E-4	4.256543584452379E-4
15	0.0	0.0	0.0	0.0011067013319576184	5.95916101823333E-4
16	0.0	0.0	0.0	0.0014472248187138088	5.95916101823333E-4
17	0.0	0.0	0.0	0.0015323556904028564	5.95916101823333E-4
18	0.0	0.0	0.0	0.0017026174337809515	6.810469735123806E-4
19	0.0	0.0	0.0	0.001787748305469999	6.810469735123806E-4
20	0.0	0.0	0.0	0.002043140920537142	6.810469735123806E-4
21	0.0	0.0	0.0	0.0024687952789823795	7.661778452014282E-4
22	0.0	0.0	0.0	0.004767328814586664	7.661778452014282E-4
23	0.0	0.0	0.0	0.008853610655660947	7.661778452014282E-4
24	0.0	0.0	0.0	0.014557379058827135	7.661778452014282E-4
25	0.0	0.0	0.0	0.01745182869625475	7.661778452014282E-4
26	0.0	0.0	0.0	0.02315559709942094	7.661778452014282E-4
27	0.0	0.0	0.0	0.029966066834544745	7.661778452014282E-4
28	0.0	0.0	0.0	0.044438315021682835	7.661778452014282E-4
29	0.0	0.0	0.0	0.06776417386448187	7.661778452014282E-4
30	0.0	0.0	0.0	0.11364971370487852	7.661778452014282E-4
31	0.0	0.0	0.0	0.20227095113317703	7.661778452014282E-4
32	0.0	0.0	0.0	0.30664139982394933	7.661778452014282E-4
33	0.0	0.0	0.0	0.4020731069873717	7.661778452014282E-4
34	0.0	0.0	0.0	0.5130837636698897	7.661778452014282E-4
35	0.0	0.0	0.0	0.6466541013500053	7.661778452014282E-4
36	0.0	0.0	0.0	0.8330907103490196	7.661778452014282E-4
37	0.0	0.0	0.0	1.0946127481777737	7.661778452014282E-4
38	0.0	0.0	0.0	1.4058512150729316	7.661778452014282E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGTCG	50	8.34145E-5	26.399899	8
TATCTCG	50	0.0025804525	21.999916	27
TATACCG	50	0.0025804525	21.999916	5
GTATTAT	95	6.854989E-7	20.845573	1
GTGATCG	75	5.4726283E-5	20.533255	9
TTCGGTC	60	0.0074121454	18.333263	12
TAATACT	280	0.0	18.07136	4
TATCGTT	75	0.0012908742	17.599932	11
GTGTATA	225	1.8189894E-12	16.62499	1
TACCGTC	240	0.0	16.499937	7
TACGCTA	255	0.0	16.392094	9
GATTTCG	125	9.440077E-6	15.839939	41
ACGCTAT	265	0.0	15.773523	10
AGTGCGG	85	0.0029799512	15.529351	10
CGAGCCG	355	0.0	15.492898	15
ATACCGT	270	0.0	15.481421	6
GATCGAA	100	4.9436896E-4	15.399941	11
TAGGGTG	200	1.2751116E-9	15.399941	5
TATAAGC	100	4.9436896E-4	15.399941	3
CTAATAC	375	0.0	15.253274	3
>>END_MODULE