FastQCFastQC Report
Thu 2 Feb 2017
SRR4062539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354040
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38130.2816017252075271No Hit
TATCAACGCAGAGTACTTTTTTTTT25650.18943310389648754No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24310.17953679359546248No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18170.13419101355942217No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15310.11306903784230894No Hit
GTACATGGAAGCAGTGGTATCAACG13980.10324658060323182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA851.7103957E-814.5328916
CGAACGT801.2894634E-714.248674
GTACTAA707.312423E-613.5621381
GTAAGAC802.0067728E-613.0578993
CGACCAT1950.012.66688810
AATCGTA604.0920867E-412.6664213
CCGACCA1900.012.5002199
CGCGTAA1001.4415491E-712.35021610
GCGTAAC1001.4415491E-712.35021611
TCGCGTA1103.8107828E-812.0911219
ACGAACG951.038281E-612.00109815
ACGTCTG1052.7167334E-711.7625467
CAATACT1302.6175258E-911.69121554
CGTACTC658.045978E-411.68818953
CTAATTC658.045978E-411.68818953
ATATACG2300.011.5658486
TCGAACG1001.936969E-611.3959853
CGGTCCA3100.011.33890910
GTCACGC600.005872606811.0839388
CGTCGTA1201.2741293E-711.08352810
Produced by FastQC (version 0.11.5)