##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062539_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1354040
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17856341023899	32.0	32.0	32.0	32.0	32.0
2	30.867718088091934	32.0	32.0	32.0	32.0	32.0
3	30.853394286727127	32.0	32.0	32.0	32.0	32.0
4	30.91669965436767	32.0	32.0	32.0	32.0	32.0
5	30.735190245487576	32.0	32.0	32.0	32.0	32.0
6	34.38309872677321	36.0	36.0	36.0	32.0	36.0
7	34.25390608844643	36.0	36.0	36.0	32.0	36.0
8	34.25770065876931	36.0	36.0	36.0	32.0	36.0
9	34.3882507163747	36.0	36.0	36.0	32.0	36.0
10	34.098178783492365	36.0	36.0	36.0	32.0	36.0
11	34.40076881037488	36.0	36.0	36.0	32.0	36.0
12	34.18583129006529	36.0	36.0	36.0	32.0	36.0
13	34.302691944108005	36.0	36.0	36.0	32.0	36.0
14	34.21590499542111	36.0	36.0	36.0	32.0	36.0
15	34.16586216064518	36.0	36.0	36.0	32.0	36.0
16	34.16029511683554	36.0	36.0	36.0	32.0	36.0
17	34.09398245251248	36.0	36.0	36.0	32.0	36.0
18	34.11694853918643	36.0	36.0	36.0	32.0	36.0
19	34.08944048920268	36.0	36.0	36.0	32.0	36.0
20	34.084905172668456	36.0	36.0	36.0	32.0	36.0
21	34.06106983545538	36.0	36.0	36.0	32.0	36.0
22	34.01500472659596	36.0	36.0	36.0	32.0	36.0
23	33.98700629228088	36.0	36.0	36.0	32.0	36.0
24	33.963113349679475	36.0	36.0	36.0	32.0	36.0
25	33.53276343387197	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	44.0
5	132.0
6	230.0
7	51.0
8	212.0
9	246.0
10	153.0
11	50.0
12	96.0
13	92.0
14	245.0
15	280.0
16	432.0
17	608.0
18	807.0
19	1177.0
20	1727.0
21	2581.0
22	3800.0
23	5626.0
24	7790.0
25	10787.0
26	14918.0
27	19858.0
28	26738.0
29	36072.0
30	46508.0
31	64144.0
32	93809.0
33	136644.0
34	310657.0
35	567518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27581968908243	17.52966184317039	11.11240774989512	26.082110717852057
2	17.21672943145369	20.357061352425447	36.255250196270744	26.170959019850116
3	19.110027880869293	24.016100740412487	28.40687605014864	28.466995328569585
4	13.109185226877388	15.57462235113976	35.45655791649955	35.85963450548331
5	15.088486356537146	36.038778257342585	33.070565966922345	15.802169419197925
6	33.616864069276865	35.17285966558545	17.123414190821702	14.086862074315986
7	29.903784828022495	30.679182004754963	20.870023420069128	18.547009747153414
8	28.25331542536717	31.9328998265135	19.933207327088216	19.880577421031116
9	27.848751596726117	14.293064294838434	18.845803567204428	39.01238054123102
10	16.425316515596382	26.406158846724598	31.13819917912605	26.030325458552966
11	37.13027475180357	21.41325010702743	22.128540870430633	19.327934270738368
12	24.74968572062412	23.967462841085556	28.361162463950308	22.92168897434001
13	29.466554081032083	19.63345094687279	25.192095542022937	25.707899430072196
14	23.74884833857589	19.724207590391558	25.128697702001045	31.39824636903151
15	25.360918343657257	26.740932864677987	22.63497377491826	25.26317501674649
16	25.770946606356254	25.714534977427956	23.65207259134174	24.86244582487405
17	24.006994790877194	25.479450029021727	25.371200834051173	25.1423543460499
18	24.889299384158498	24.712710826623756	26.177330998538785	24.220658790678964
19	25.41912764656249	25.132942830330858	25.628257016149846	23.819672506956806
20	25.70469843321475	24.14759976249402	25.216598503239084	24.93110330105214
21	26.802504475711363	24.111309702957705	24.547677695539228	24.538508125791708
22	25.78400794639879	24.40670118685649	24.842924754023308	24.96636611272141
23	24.59558317815343	24.297031343135675	25.36544312428542	25.741942354425472
24	24.58897828787789	25.171641128793702	25.221484351890194	25.017896231438208
25	24.747169242054948	24.551574812465983	25.34053396909534	25.360721976383726
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	199.0
1	199.0
2	578.0
3	957.0
4	957.0
5	957.0
6	2406.0
7	3855.0
8	3855.0
9	3855.0
10	4072.5
11	4290.0
12	4290.0
13	4290.0
14	4443.5
15	4597.0
16	4597.0
17	4597.0
18	7547.0
19	10497.0
20	10497.0
21	10497.0
22	16146.5
23	21796.0
24	21796.0
25	21796.0
26	31447.0
27	41098.0
28	41098.0
29	41098.0
30	49735.0
31	58372.0
32	58372.0
33	58372.0
34	71211.0
35	84050.0
36	84050.0
37	84050.0
38	94709.0
39	105368.0
40	105368.0
41	105368.0
42	120571.0
43	135774.0
44	135774.0
45	135774.0
46	155176.0
47	174578.0
48	174578.0
49	174578.0
50	175890.5
51	177203.0
52	177203.0
53	177203.0
54	165391.5
55	153580.0
56	153580.0
57	153580.0
58	140646.5
59	127713.0
60	127713.0
61	127713.0
62	113814.5
63	99916.0
64	99916.0
65	99916.0
66	83854.5
67	67793.0
68	67793.0
69	67793.0
70	52090.0
71	36387.0
72	36387.0
73	36387.0
74	29129.0
75	21871.0
76	21871.0
77	21871.0
78	17718.0
79	13565.0
80	13565.0
81	13565.0
82	9772.5
83	5980.0
84	5980.0
85	5980.0
86	4425.5
87	2871.0
88	2871.0
89	2871.0
90	1915.5
91	960.0
92	960.0
93	960.0
94	577.5
95	195.0
96	195.0
97	195.0
98	385.0
99	575.0
100	575.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008271542938170217
2	0.0031756816637617796
3	0.004800449026616643
4	0.014992171575433519
5	0.03677882485007829
6	0.04652742902720747
7	0.06846178842574814
8	0.08840211515169419
9	0.09394109479779032
10	0.11661398481581045
11	0.11690939706360225
12	0.12850432778943016
13	0.117278662373342
14	0.12156213996632301
15	0.11210894803698561
16	0.10945023780685947
17	0.11853416442645713
18	0.12879974003722194
19	0.13049836046202476
20	0.12207911139995864
21	0.1285781808513781
22	0.1463029157188857
23	0.13633275235591266
24	0.13293551150630706
25	0.12939056453280554
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1354040.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.839071646728065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.56371672929951	46.36005938950088
2	11.881620338711011	13.50680539422433
3	3.0429462266072766	5.188747157738155
4	1.1665182974202226	2.6521526833714915
5	0.5823195547786574	1.6549251447677449
6	0.35350522392976896	1.205574525026207
7	0.21710812266430993	0.8638156897442337
8	0.1632276922179193	0.7422168394163514
9	0.11863771252514249	0.6068931697998451
>10	0.7108630558869949	7.8174091989511645
>50	0.09782262518626492	3.9045100519727676
>100	0.0924794991451501	10.854148549809633
>500	0.006893673891103825	2.618755305172036
>1k	0.002341247736601299	2.0239869005051894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3813	0.2816017252075271	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2565	0.18943310389648754	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2431	0.17953679359546248	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1817	0.13419101355942217	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1531	0.11306903784230894	No Hit
GTACATGGAAGCAGTGGTATCAACG	1398	0.10324658060323182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.2155918584384508E-4	0.0
10	0.0	0.0	0.0	2.2155918584384508E-4	0.0
11	0.0	0.0	0.0	2.2155918584384508E-4	0.0
12	0.0	0.0	0.0	2.2155918584384508E-4	0.0
13	0.0	0.0	0.0	3.692653097397418E-4	7.385306194794836E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	85	1.7103957E-8	14.53289	16
CGAACGT	80	1.2894634E-7	14.24867	4
GTACTAA	70	7.312423E-6	13.562138	1
GTAAGAC	80	2.0067728E-6	13.057899	3
CGACCAT	195	0.0	12.666888	10
AATCGTA	60	4.0920867E-4	12.66642	13
CCGACCA	190	0.0	12.500219	9
CGCGTAA	100	1.4415491E-7	12.350216	10
GCGTAAC	100	1.4415491E-7	12.350216	11
TCGCGTA	110	3.8107828E-8	12.091121	9
ACGAACG	95	1.038281E-6	12.001098	15
ACGTCTG	105	2.7167334E-7	11.762546	7
CAATACT	130	2.6175258E-9	11.6912155	4
CGTACTC	65	8.045978E-4	11.6881895	3
CTAATTC	65	8.045978E-4	11.6881895	3
ATATACG	230	0.0	11.565848	6
TCGAACG	100	1.936969E-6	11.395985	3
CGGTCCA	310	0.0	11.338909	10
GTCACGC	60	0.0058726068	11.083938	8
CGTCGTA	120	1.2741293E-7	11.083528	10
>>END_MODULE