FastQCFastQC Report
Thu 2 Feb 2017
SRR4062539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354040
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13600.10044016424920978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAA808.9897614E-519.24995210
CGTTGCG600.00741265818.33328637
ATACCCG600.00741265818.3332865
TAGGACG1355.5271812E-817.9258824
GGCGTTA3850.016.57138842
TCTAGTA1206.405582E-616.5005682
GAATAGA1501.8365608E-716.1344831
ATTATCC1501.838107E-716.1332933
TACGACG3450.015.9419885
GTATCAA37050.015.9147651
ACGCTAT3350.015.76115510
ACGGTAT3350.015.7611559
GTATTAG4250.015.530521
ATCTAGT850.002978771215.53051951
GTACTAA1004.9415586E-415.4010981
GACGGTA3450.015.3043098
CGACGGT3600.015.2777397
GTCTTAC1301.3682604E-515.2318561
CGCGGGA3200.015.12496344
CGCTATT3650.015.06845511
Produced by FastQC (version 0.11.5)