FastQCFastQC Report
Thu 2 Feb 2017
SRR4062538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837063
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30590.36544441696742064No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19450.23236004936307064No Hit
TATCAACGCAGAGTACTTTTTTTTT18350.21921886405204863No Hit
GTACATGGAAGCAGTGGTATCAACG15430.18433499031733572No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15020.1794369121559548No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12710.15184042300280864No Hit
TCCATGTACTCTGCGTTGATACCAC12360.14765913676748346No Hit
GGTATCAACGCAGAGTACTTTTTTT10060.12018211293534657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCC350.002166198816.28894617
CGAACGA350.00216706516.2879716
GTATTAA508.758309E-515.1893841
TCTATAC456.7751476E-414.7727553
ACGAACG400.00527364614.25112215
CGGTCCA1257.2759576E-1213.68107610
ATTCCGA500.00149764713.3010486
GTGTTAC500.001506447813.2907111
GATCGCA655.442585E-513.154881511
TTAATCC604.0899226E-412.6661484
GTATTGA751.4872961E-512.657821
TCTCGTC550.003063226112.09258412
ATACCAT550.003064599412.091866
CTCGTCT550.003065972812.09113713
TAAGTTG550.003065972812.0911375
ATTACCC550.003074225512.0867993
CGAGCCG951.0368676E-612.00094415
GTCCTAC1900.011.9916171
CACTCTA1353.6925485E-1011.9639049
ACACTCT1353.6925485E-1011.9639048
Produced by FastQC (version 0.11.5)