FastQCFastQC Report
Thu 2 Feb 2017
SRR4062538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837063
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC300.00574413229.33321410
TACTAGA803.6134716E-621.999912
CGAATGT1452.9467628E-1019.72405833
TATACTG1452.9467628E-1019.7240585
CGTCTTA901.0013915E-519.55547515
TAGGACG3050.018.7540234
ATACACT2950.018.6439934
ACGAACG1102.7944498E-617.99992815
TTACCGC1601.1004886E-917.87492828
TTAATCC1254.84828E-717.5999284
AATCCCG1408.3633495E-817.28564519
GGCTAAT902.2113859E-417.1141091
ATAACGC1802.8558134E-1017.1110423
CGCAATA1551.4371835E-817.03218836
GTATAGA1307.296785E-716.9260431
GATATAC2500.016.7229291
ACCGTCG1451.2479177E-716.6895898
TATGGCG3300.016.666616
TAGCGGC1602.1263986E-816.49993330
TCTAGCG1602.1263986E-816.49993328
Produced by FastQC (version 0.11.5)