##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062538_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	837063
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18595494007022	32.0	32.0	32.0	32.0	32.0
2	31.386152535711172	32.0	32.0	32.0	32.0	32.0
3	31.491247373256254	32.0	32.0	32.0	32.0	32.0
4	31.603402611272987	32.0	32.0	32.0	32.0	32.0
5	31.528307905139755	32.0	32.0	32.0	32.0	32.0
6	35.126842304581615	36.0	36.0	36.0	36.0	36.0
7	35.13303538682273	36.0	36.0	36.0	36.0	36.0
8	35.084340127326136	36.0	36.0	36.0	36.0	36.0
9	35.193342675521436	36.0	36.0	36.0	36.0	36.0
10	35.086461831427265	36.0	36.0	36.0	36.0	36.0
11	35.20978827161157	36.0	36.0	36.0	36.0	36.0
12	35.117216983667895	36.0	36.0	36.0	36.0	36.0
13	35.163463204083804	36.0	36.0	36.0	36.0	36.0
14	35.12243403423637	36.0	36.0	36.0	36.0	36.0
15	35.0956391573872	36.0	36.0	36.0	36.0	36.0
16	35.10275929051935	36.0	36.0	36.0	36.0	36.0
17	35.083312725565456	36.0	36.0	36.0	36.0	36.0
18	35.08982239090726	36.0	36.0	36.0	36.0	36.0
19	35.06955270989161	36.0	36.0	36.0	36.0	36.0
20	35.054496495484806	36.0	36.0	36.0	36.0	36.0
21	35.044011024259824	36.0	36.0	36.0	36.0	36.0
22	35.031327391128265	36.0	36.0	36.0	36.0	36.0
23	34.99386784507259	36.0	36.0	36.0	36.0	36.0
24	34.97332578312505	36.0	36.0	36.0	36.0	36.0
25	34.9627387663772	36.0	36.0	36.0	36.0	36.0
26	34.909366439563094	36.0	36.0	36.0	32.0	36.0
27	34.897711402845424	36.0	36.0	36.0	32.0	36.0
28	34.875437093743244	36.0	36.0	36.0	32.0	36.0
29	34.8506468449806	36.0	36.0	36.0	32.0	36.0
30	34.82488534315816	36.0	36.0	36.0	32.0	36.0
31	34.817422344554714	36.0	36.0	36.0	32.0	36.0
32	34.78874708355285	36.0	36.0	36.0	32.0	36.0
33	34.751243335328404	36.0	36.0	36.0	32.0	36.0
34	34.72693572646264	36.0	36.0	36.0	32.0	36.0
35	34.68122829464449	36.0	36.0	36.0	32.0	36.0
36	34.65197123752932	36.0	36.0	36.0	32.0	36.0
37	34.64200424579751	36.0	36.0	36.0	32.0	36.0
38	34.59596470038695	36.0	36.0	36.0	32.0	36.0
39	34.575571970090664	36.0	36.0	36.0	32.0	36.0
40	34.55586138677734	36.0	36.0	36.0	32.0	36.0
41	34.52082579208494	36.0	36.0	36.0	32.0	36.0
42	34.48708520147229	36.0	36.0	36.0	32.0	36.0
43	34.468121276415275	36.0	36.0	36.0	32.0	36.0
44	34.41997555739532	36.0	36.0	36.0	32.0	36.0
45	34.35758837745785	36.0	36.0	36.0	32.0	36.0
46	34.34261101016291	36.0	36.0	36.0	32.0	36.0
47	34.2933685994961	36.0	36.0	36.0	32.0	36.0
48	34.249871276116615	36.0	36.0	36.0	32.0	36.0
49	34.230274184858246	36.0	36.0	36.0	32.0	36.0
50	33.71301682191185	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	9.0
21	36.0
22	96.0
23	270.0
24	668.0
25	1520.0
26	3181.0
27	5703.0
28	9485.0
29	14074.0
30	19627.0
31	28112.0
32	40440.0
33	64720.0
34	139493.0
35	509626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.44869264334087	17.947418980209235	11.518526660899736	26.08536171555016
2	15.826742491218848	20.7705909058326	37.29289622709135	26.109770375857206
3	19.17931612863147	24.707367948218643	28.375333608901656	27.73798231424824
4	12.367303736162913	15.839447973985202	36.04213307974797	35.75111521010391
5	13.972902875888673	37.26171148408184	33.58086547846458	15.1845201615649
6	33.79104698712994	35.55472194910992	16.989722349320658	13.664508714439483
7	29.57423754245499	30.72839200872575	21.016817133238476	18.680553315580788
8	27.939713020405872	33.182090236935565	19.68071698307057	19.19747975958799
9	27.53018589998602	14.090337286440805	18.699667766942273	39.6798090466309
10	15.524279534515323	27.368429855339443	32.42802513072493	24.679265479420305
11	37.165900296632394	21.437812924475217	22.191400169401827	19.204886609490565
12	24.235212881228772	24.163533688623197	29.489775560501418	22.11147786964661
13	29.80289416686677	19.889064502910774	25.380885309707875	24.927156020514584
14	23.078788573858837	19.821566596540524	25.942491783772546	31.15715304582809
15	24.861569559280483	27.54488013447017	23.26372089078122	24.329829415468133
16	24.920824358501093	26.114880241988953	24.90601065869594	24.058284740814013
17	23.37135914501059	25.954557781194488	26.204957094029957	24.469125979764964
18	24.112402531231222	25.209930435343576	27.395906879171577	23.28176015425362
19	24.91915184400696	25.464749965056395	26.214155923747672	23.401942267188968
20	25.016277149987516	24.681535320519483	26.58832130914877	23.713866220344226
21	25.99983513785701	24.65680599907056	25.37323952916328	23.970119333909157
22	24.77615185475884	25.139684826590113	25.89374993280076	24.190413385850288
23	23.977167787848703	24.833136812880273	26.261464190867358	24.928231208403666
24	23.9783624410588	25.52257118042489	26.28738816552637	24.21167821298994
25	24.36327970535073	25.039453422263318	26.28786602681041	24.309400845575542
26	23.618652359499823	25.50297886777937	26.85532630160454	24.02304247111627
27	24.33998396775392	25.70153023129681	26.146060690772376	23.812425110176893
28	23.738715007114163	25.354961335048852	26.520106610852466	24.386217046984516
29	23.712313171171107	25.379451725855763	26.59405564455722	24.314179458415914
30	23.944434289892158	25.58146758368247	26.658805848544255	23.815292277881113
31	24.049563772380335	25.350421652850503	26.101500126035916	24.49851444873325
32	23.717211249332486	25.65111586583089	26.133397366745392	24.49827551809123
33	23.637647345540298	25.23943836963287	26.54196876459717	24.580945520229662
34	23.915284751565892	25.556379866270518	26.81972563594377	23.708609746219818
35	24.58632145967508	25.430702348568744	26.438033935319087	23.54494225643709
36	23.651863718740408	25.84464968586594	26.27914505837673	24.224341537016926
37	24.423729157781434	25.576211109558063	25.95897799807183	24.041081734588676
38	23.875383334348786	25.517075775658466	26.22896962355283	24.378571266439923
39	24.0401260120206	25.37921279521374	26.16792284451708	24.412738348248578
40	24.63171828165861	25.339430843317643	26.321913643298057	23.70693723172569
41	23.633585524625982	25.556977192875568	26.824743179426157	23.98469410307229
42	24.492421717361776	25.876905322538445	26.231836791257052	23.398836168842728
43	23.58030399145584	25.183409134079515	26.601462494459792	24.63482438000485
44	23.402897989757044	25.675367325995772	26.41999467184668	24.5017400124005
45	23.558680768353156	25.77894375931083	26.328364770632557	24.334010701703455
46	23.40026975269484	25.354841869727846	26.404942041399515	24.839946336177803
47	23.78231984928255	25.34886860367738	26.578166756862988	24.290644790177083
48	23.91695726606002	26.031015586640432	25.727334740634817	24.32469240666473
49	23.31963066101357	25.84990615999035	26.289777471946557	24.54068570704953
50	23.12597737565751	26.441737360270373	25.805226129932873	24.627059134139245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	51.0
2	55.0
3	186.5
4	318.0
5	362.0
6	406.0
7	320.5
8	235.0
9	342.5
10	450.0
11	671.0
12	892.0
13	1419.5
14	1947.0
15	2648.0
16	3349.0
17	3691.5
18	4034.0
19	4313.5
20	4593.0
21	5091.5
22	5590.0
23	6013.5
24	6437.0
25	7062.0
26	7687.0
27	9264.0
28	10841.0
29	12162.5
30	13484.0
31	14978.0
32	16472.0
33	18862.0
34	21252.0
35	24350.0
36	27448.0
37	30996.5
38	34545.0
39	36261.5
40	37978.0
41	41042.5
42	44107.0
43	45535.5
44	46964.0
45	51298.5
46	55633.0
47	58354.0
48	61075.0
49	61982.0
50	62889.0
51	60489.0
52	58089.0
53	56327.5
54	54566.0
55	54359.5
56	54153.0
57	52187.5
58	50222.0
59	45828.0
60	41434.0
61	36328.0
62	31222.0
63	27349.5
64	23477.0
65	20102.5
66	16728.0
67	14581.5
68	12435.0
69	11103.0
70	9771.0
71	7677.5
72	5584.0
73	4923.0
74	4262.0
75	3182.0
76	2102.0
77	1849.0
78	1596.0
79	1353.0
80	1110.0
81	900.0
82	690.0
83	559.0
84	428.0
85	343.5
86	259.0
87	206.5
88	154.0
89	91.0
90	28.0
91	20.0
92	12.0
93	8.0
94	4.0
95	3.5
96	3.0
97	3.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012782789347994117
2	0.005137008803399505
3	5.973266050464541E-4
4	1.1946532100929081E-4
5	0.0
6	4.7786128403716325E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	837063.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.81964110587676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.16259875906327	54.07912762647701
2	11.898880819399862	15.663601301889992
3	2.934094040837089	5.7936305021636665
4	1.0572812202414275	2.783594818570968
5	0.5156008675179018	1.69683320269535
6	0.2911135022698835	1.1496591744287143
7	0.1895584088916248	0.8733666509293448
8	0.1363382637594456	0.7178988471716058
9	0.10606943418064811	0.6283306881066308
>10	0.5925744530662146	7.267801942320211
>50	0.06606839144739113	3.1001943995088936
>100	0.04836185870017347	5.67359261491834
>500	0.001459980625123125	0.5723682308192638
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.1946532100929081E-4	0.0
14	0.0	0.0	0.0	3.5839596302787247E-4	0.0
15	0.0	0.0	0.0	4.7786128403716325E-4	0.0
16	0.0	0.0	0.0	4.7786128403716325E-4	0.0
17	0.0	0.0	0.0	4.7786128403716325E-4	0.0
18	0.0	0.0	0.0	7.167919260557449E-4	0.0
19	0.0	0.0	0.0	9.557225680743265E-4	0.0
20	0.0	0.0	0.0	0.0017919798151393622	0.0
21	0.0	0.0	0.0	0.002389306420185816	0.0
22	0.0	0.0	0.0	0.0037034249512880152	0.0
23	0.0	0.0	0.0	0.006690057976520286	0.0
24	0.0	0.0	0.0	0.010632413569826882	0.0
25	0.0	0.0	0.0	0.014335838521114898	0.0
26	0.0	0.0	0.0	0.01911445136148653	0.0
27	0.0	0.0	0.0	0.024131994843876747	0.0
28	0.0	0.0	0.0	0.03751211079691732	0.0
29	0.0	0.0	0.0	0.051609018676013634	0.0
30	0.0	0.0	0.0	0.08398412066953144	0.0
31	0.0	0.0	0.0	0.14789806740950204	0.0
32	0.0	0.0	0.0	0.22447533817645746	0.0
33	0.0	0.0	0.0	0.3018888661904779	0.0
34	0.0	0.0	0.0	0.3928019754785482	0.0
35	0.0	0.0	0.0	0.49745359668268696	0.0
36	0.0	0.0	0.0	0.6374669529055758	0.0
37	0.0	0.0	0.0	0.8364961777070543	0.0
38	0.0	0.0	0.0	1.0981252307174012	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTTC	30	0.005744132	29.333214	10
TACTAGA	80	3.6134716E-6	21.99991	2
CGAATGT	145	2.9467628E-10	19.724058	33
TATACTG	145	2.9467628E-10	19.724058	5
CGTCTTA	90	1.0013915E-5	19.555475	15
TAGGACG	305	0.0	18.754023	4
ATACACT	295	0.0	18.643993	4
ACGAACG	110	2.7944498E-6	17.999928	15
TTACCGC	160	1.1004886E-9	17.874928	28
TTAATCC	125	4.84828E-7	17.599928	4
AATCCCG	140	8.3633495E-8	17.285645	19
GGCTAAT	90	2.2113859E-4	17.114109	1
ATAACGC	180	2.8558134E-10	17.111042	3
CGCAATA	155	1.4371835E-8	17.032188	36
GTATAGA	130	7.296785E-7	16.926043	1
GATATAC	250	0.0	16.722929	1
ACCGTCG	145	1.2479177E-7	16.689589	8
TATGGCG	330	0.0	16.6666	16
TAGCGGC	160	2.1263986E-8	16.499933	30
TCTAGCG	160	2.1263986E-8	16.499933	28
>>END_MODULE