FastQCFastQC Report
Thu 2 Feb 2017
SRR4062537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143066
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG60940.5331275709364114No Hit
TCCATGTACTCTGCGTTGATACCAC53920.4717137943040909No Hit
GTATCAACGCAGAGTACTTTTTTTT36740.32141626117826966No Hit
GAGTACATGGAAGCAGTGGTATCAA28710.2511665993039772No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28010.2450427184432045No Hit
CATGTACTCTGCGTTGATACCACTG26610.23279495672165912No Hit
TATCAACGCAGAGTACTTTTTTTTT25020.21888499876647544No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20790.181879261564949No Hit
CCCATGTACTCTGCGTTGATACCAC18640.16307019892114716No Hit
GCTTCCATGTACTCTGCGTTGATAC17650.15440928170376864No Hit
GTACATGGGAAGCAGTGGTATCAAC17460.15274708547013033No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16210.14181158393303622No Hit
GCGTTGATACCACTGCTTCCATGTA15850.13866215949035313No Hit
GCGCAAGACGGACCAGAGCGAAAGC15420.13490034696159275No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14890.13026369430986487No Hit
CATGGAAGCAGTGGTATCAACGCAG13870.12134032505559608No Hit
GAATAACGCCGCCGCATCGCCAGTC13510.11819090061291299No Hit
GTATCTGATCGTCTTCGAACCTCCG12830.11224198777673379No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12630.11049230753079875No Hit
GAATAGGACCGCGGTTCTATTTTGT12520.10952998339553448No Hit
GGTATCAACGCAGAGTACTTTTTTT12330.10786778716189616No Hit
GAACTACGACGGTATCTGATCGTCT12300.10760533512500589No Hit
ACTCTGCGTTGATACCACTGCTTCC12070.1055932028421806No Hit
GTATCAACGCAGAGTACATGGAAGC11740.10270623043638774No Hit
GATTAAGAGGGACGGCCGGGGGCAT11680.10218132636260722No Hit
GTACATGGGGTGGTATCAACGCAAA11520.10078158216585918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGC601.4654142E-615.83391914
CGCCAGT2300.015.284512518
GCATCGC2500.013.68050514
TGCCGAG500.001497221313.30223912
ATCGCCA2500.013.3004916
ACATAGG500.00150217213.2964143
GCGTTAT1301.4551915E-1113.1451181
CGCAAGA3050.013.0772942
CGCATCG2550.013.04026713
TGTAGGA6600.012.9499182
CGACCCG2100.012.6671345
ATATACG2050.012.5132066
CGTTATT1301.9826984E-1012.4186392
TACGCTA2150.012.3741759
AATCGCT853.941026E-612.29403315
CGGACCA3350.012.1960829
GTATTAG3050.012.1394811
AAGACGG3450.012.1163895
TCGCCAG2750.012.09241417
CAAGACG3550.012.040064
Produced by FastQC (version 0.11.5)