##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062537_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143066
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16281999464598	32.0	32.0	32.0	32.0	32.0
2	30.920614382721556	32.0	32.0	32.0	32.0	32.0
3	30.90597130874333	32.0	32.0	32.0	32.0	32.0
4	30.949123672648824	32.0	32.0	32.0	32.0	32.0
5	30.81759495952115	32.0	32.0	32.0	32.0	32.0
6	34.462402870875344	36.0	36.0	36.0	32.0	36.0
7	34.31981967795385	36.0	36.0	36.0	32.0	36.0
8	34.3215317400745	36.0	36.0	36.0	32.0	36.0
9	34.42392915194748	36.0	36.0	36.0	32.0	36.0
10	34.18235692427209	36.0	36.0	36.0	32.0	36.0
11	34.450951213665704	36.0	36.0	36.0	32.0	36.0
12	34.26325601496327	36.0	36.0	36.0	32.0	36.0
13	34.36347245040969	36.0	36.0	36.0	32.0	36.0
14	34.28373689708206	36.0	36.0	36.0	32.0	36.0
15	34.24828050173831	36.0	36.0	36.0	32.0	36.0
16	34.23058161121055	36.0	36.0	36.0	32.0	36.0
17	34.1709455097081	36.0	36.0	36.0	32.0	36.0
18	34.195412163427136	36.0	36.0	36.0	32.0	36.0
19	34.168051538581324	36.0	36.0	36.0	32.0	36.0
20	34.15133334383142	36.0	36.0	36.0	32.0	36.0
21	34.10171678625731	36.0	36.0	36.0	32.0	36.0
22	34.0690616289873	36.0	36.0	36.0	32.0	36.0
23	34.03167621117241	36.0	36.0	36.0	32.0	36.0
24	34.00336026091232	36.0	36.0	36.0	32.0	36.0
25	33.56249507902431	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	36.0
5	105.0
6	192.0
7	47.0
8	177.0
9	193.0
10	125.0
11	45.0
12	98.0
13	59.0
14	236.0
15	277.0
16	393.0
17	547.0
18	721.0
19	1056.0
20	1665.0
21	2256.0
22	3336.0
23	4740.0
24	6467.0
25	9111.0
26	12252.0
27	15849.0
28	21347.0
29	28495.0
30	37381.0
31	51586.0
32	74810.0
33	110310.0
34	254053.0
35	505094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.642947322004304	17.46836949227578	11.371234239428572	25.51744894629135
2	16.21731808140679	20.37120797882811	37.25998993897771	26.151484000787384
3	19.6266203798887	24.09260038547041	28.226039987086825	28.054739247554068
4	12.59811586049283	15.413125629424565	35.52216318184562	36.466595328236984
5	14.400856931324727	36.61569885584966	33.39065461782018	15.592789595005435
6	34.07696690627836	34.741099459713986	17.210643275570373	13.971290358437274
7	29.765318878488966	30.637844519379925	20.86454186057098	18.73229474156013
8	28.144465478081337	32.85934684428318	19.988687366800747	19.007500310834736
9	27.13010492247716	14.595012548314953	19.126241477566275	39.14864105164161
10	16.07051987783655	26.778127241626663	31.60808548989314	25.543267390643653
11	37.007245056685214	21.21272369287686	22.150937513137798	19.62909373730013
12	24.681960366256924	23.90060292413387	29.033297915299798	22.384138794309404
13	29.915689496309117	19.477075180515214	25.400619409808954	25.206615913366708
14	23.44267191505873	20.358015315985263	24.990912538769518	31.208400230186488
15	24.986644012576523	27.864530876415515	23.171017945192283	23.97780716581568
16	25.25748998066198	25.906207085003224	25.133824724643368	23.70247820969143
17	23.344909586977373	26.31485366184785	26.369771525919568	23.97046522525521
18	24.563909945673785	24.20337029244356	28.304552645271375	22.928167116611277
19	24.9587203980361	25.905370071084132	25.730528510299095	23.405381020580673
20	24.976482562060635	24.275338835041595	26.764771315907947	23.983407286989824
21	25.755886220038942	24.880803989463978	24.55634907634589	24.80696071415119
22	24.875213453553645	25.70212967286956	25.194572387586973	24.22808448598982
23	23.255306660592733	25.194876871457485	26.068734723913483	25.481081744036306
24	23.974207134822997	26.062234353811814	25.966575446145434	23.99698306521975
25	24.46741415557113	25.128416257883675	25.68999649614576	24.714173090399438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	214.0
2	562.0
3	910.0
4	910.0
5	910.0
6	2451.5
7	3993.0
8	3993.0
9	3993.0
10	4073.5
11	4154.0
12	4154.0
13	4154.0
14	3838.5
15	3523.0
16	3523.0
17	3523.0
18	5800.5
19	8078.0
20	8078.0
21	8078.0
22	13155.0
23	18232.0
24	18232.0
25	18232.0
26	27640.0
27	37048.0
28	37048.0
29	37048.0
30	46596.5
31	56145.0
32	56145.0
33	56145.0
34	65888.0
35	75631.0
36	75631.0
37	75631.0
38	83245.0
39	90859.0
40	90859.0
41	90859.0
42	104047.5
43	117236.0
44	117236.0
45	117236.0
46	143953.0
47	170670.0
48	170670.0
49	170670.0
50	161031.0
51	151392.0
52	151392.0
53	151392.0
54	134625.5
55	117859.0
56	117859.0
57	117859.0
58	108136.5
59	98414.0
60	98414.0
61	98414.0
62	88015.5
63	77617.0
64	77617.0
65	77617.0
66	64163.5
67	50710.0
68	50710.0
69	50710.0
70	38679.0
71	26648.0
72	26648.0
73	26648.0
74	20999.5
75	15351.0
76	15351.0
77	15351.0
78	13057.0
79	10763.0
80	10763.0
81	10763.0
82	7451.5
83	4140.0
84	4140.0
85	4140.0
86	3125.5
87	2111.0
88	2111.0
89	2111.0
90	1410.5
91	710.0
92	710.0
93	710.0
94	423.5
95	137.0
96	137.0
97	137.0
98	329.0
99	521.0
100	521.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009010853266565536
2	0.0035868445041668633
3	0.00411174857794738
4	0.013734989930590184
5	0.03359386072195306
6	0.0457541384312017
7	0.06561300922256458
8	0.08573433205081772
9	0.09448273328049299
10	0.11521644419482338
11	0.11565386425630717
12	0.12798910999014929
13	0.11670367240386818
14	0.12099038900640909
15	0.10987991944472147
16	0.11145463166606304
17	0.11906574073588053
18	0.126326913756511
19	0.1278141419655558
20	0.11897825672358377
21	0.1276391739409623
22	0.1502100491135245
23	0.13787480337968236
24	0.1321008585680967
25	0.12825156202703955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1143066.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.0539703196666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7925530832603	41.247806071628595
2	11.962333886775923	12.214492784187998
3	3.316220223378326	5.079186265735055
4	1.27091150724315	2.595403134788581
5	0.6412449930154124	1.6369051420521838
6	0.39985698755574484	1.2248572064869374
7	0.2674544553252725	0.9558228276827343
8	0.1809258899283735	0.7389588011569957
9	0.13660153586657142	0.627664568197753
>10	0.7915592677896208	7.761482963652108
>50	0.10166509292766067	3.647345495025385
>100	0.1220460215269605	13.081110392282453
>500	0.010799015367238792	3.776890918116449
>1k	0.005485214154787957	4.406897816478753
>5k	3.428258846742473E-4	1.0051756125280489
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	6094	0.5331275709364114	No Hit
TCCATGTACTCTGCGTTGATACCAC	5392	0.4717137943040909	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3674	0.32141626117826966	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2871	0.2511665993039772	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2801	0.2450427184432045	No Hit
CATGTACTCTGCGTTGATACCACTG	2661	0.23279495672165912	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2502	0.21888499876647544	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2079	0.181879261564949	No Hit
CCCATGTACTCTGCGTTGATACCAC	1864	0.16307019892114716	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1765	0.15440928170376864	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1746	0.15274708547013033	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1621	0.14181158393303622	No Hit
GCGTTGATACCACTGCTTCCATGTA	1585	0.13866215949035313	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1542	0.13490034696159275	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1489	0.13026369430986487	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1387	0.12134032505559608	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1351	0.11819090061291299	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1283	0.11224198777673379	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1263	0.11049230753079875	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1252	0.10952998339553448	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1233	0.10786778716189616	No Hit
GAACTACGACGGTATCTGATCGTCT	1230	0.10760533512500589	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1207	0.1055932028421806	No Hit
GTATCAACGCAGAGTACATGGAAGC	1174	0.10270623043638774	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1168	0.10218132636260722	No Hit
GTACATGGGGTGGTATCAACGCAAA	1152	0.10078158216585918	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7496802459350555E-4
2	0.0	0.0	0.0	0.0	1.7496802459350555E-4
3	0.0	0.0	0.0	8.748401229675277E-5	1.7496802459350555E-4
4	0.0	0.0	0.0	8.748401229675277E-5	1.7496802459350555E-4
5	0.0	0.0	0.0	8.748401229675277E-5	1.7496802459350555E-4
6	0.0	0.0	0.0	8.748401229675277E-5	1.7496802459350555E-4
7	0.0	0.0	0.0	8.748401229675277E-5	1.7496802459350555E-4
8	0.0	0.0	0.0	8.748401229675277E-5	1.7496802459350555E-4
9	0.0	0.0	0.0	1.7496802459350555E-4	1.7496802459350555E-4
10	0.0	0.0	0.0	1.7496802459350555E-4	1.7496802459350555E-4
11	0.0	0.0	0.0	1.7496802459350555E-4	1.7496802459350555E-4
12	0.0	0.0	0.0	1.7496802459350555E-4	2.624520368902583E-4
13	0.0	0.0	0.0	2.624520368902583E-4	3.499360491870111E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGC	60	1.4654142E-6	15.833919	14
CGCCAGT	230	0.0	15.2845125	18
GCATCGC	250	0.0	13.680505	14
TGCCGAG	50	0.0014972213	13.302239	12
ATCGCCA	250	0.0	13.30049	16
ACATAGG	50	0.001502172	13.296414	3
GCGTTAT	130	1.4551915E-11	13.145118	1
CGCAAGA	305	0.0	13.077294	2
CGCATCG	255	0.0	13.040267	13
TGTAGGA	660	0.0	12.949918	2
CGACCCG	210	0.0	12.667134	5
ATATACG	205	0.0	12.513206	6
CGTTATT	130	1.9826984E-10	12.418639	2
TACGCTA	215	0.0	12.374175	9
AATCGCT	85	3.941026E-6	12.294033	15
CGGACCA	335	0.0	12.196082	9
GTATTAG	305	0.0	12.139481	1
AAGACGG	345	0.0	12.116389	5
TCGCCAG	275	0.0	12.092414	17
CAAGACG	355	0.0	12.04006	4
>>END_MODULE