FastQCFastQC Report
Thu 2 Feb 2017
SRR4062537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143066
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA28850.2523913754761317No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25850.22614617178710592No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14520.127026785854885No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14500.1268518178302915No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA12690.11101721160457925No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12580.11005488746931498No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12300.10760533512500589No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12060.10550571882988384No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC11600.1014814542642332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACG300.00574502529.3331852
TTAGGAC5900.021.2541333
GACGTGA4900.021.1019367
GATATAC3600.020.782221
TCTATAC1301.6243575E-920.307593
TAGGACG8450.020.0472374
TATGTCG550.004483593619.999916
TAGTACT1152.021261E-719.1303394
CTAGCGG3700.019.02693229
CGTCTTA1759.094947E-1218.8570515
TTTAGGA6900.018.8115022
AGGACGT8700.018.7125515
TGTAGGA10000.018.6999072
GGACGTG8850.018.6439766
TAGCGGC3900.018.61529430
CGCAATA3550.018.59145536
TAGGGTG1203.1617674E-718.333245
CTGTAGG9500.018.2986491
TCTAGCG3850.018.28562428
CCGGTCC4450.017.7976659
Produced by FastQC (version 0.11.5)