##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062537_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143066
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252474485287813	32.0	32.0	32.0	32.0	32.0
2	31.468349159191156	32.0	32.0	32.0	32.0	32.0
3	31.5450227720884	32.0	32.0	32.0	32.0	32.0
4	31.63542962523599	32.0	32.0	32.0	32.0	32.0
5	31.584148246907876	32.0	32.0	32.0	32.0	32.0
6	35.19621876602051	36.0	36.0	36.0	36.0	36.0
7	35.20506514934396	36.0	36.0	36.0	36.0	36.0
8	35.154160827108846	36.0	36.0	36.0	36.0	36.0
9	35.25359428064521	36.0	36.0	36.0	36.0	36.0
10	35.155132774485466	36.0	36.0	36.0	36.0	36.0
11	35.261305121489045	36.0	36.0	36.0	36.0	36.0
12	35.17970090965876	36.0	36.0	36.0	36.0	36.0
13	35.21091433040612	36.0	36.0	36.0	36.0	36.0
14	35.16670340995183	36.0	36.0	36.0	36.0	36.0
15	35.14817779550787	36.0	36.0	36.0	36.0	36.0
16	35.15677135003578	36.0	36.0	36.0	36.0	36.0
17	35.12988488853662	36.0	36.0	36.0	36.0	36.0
18	35.14045120754182	36.0	36.0	36.0	36.0	36.0
19	35.10956847636095	36.0	36.0	36.0	36.0	36.0
20	35.093154725973825	36.0	36.0	36.0	36.0	36.0
21	35.077523957496766	36.0	36.0	36.0	36.0	36.0
22	35.06049956870382	36.0	36.0	36.0	36.0	36.0
23	35.01719498261693	36.0	36.0	36.0	36.0	36.0
24	34.99195934442981	36.0	36.0	36.0	36.0	36.0
25	34.968346534670786	36.0	36.0	36.0	36.0	36.0
26	34.9256210927453	36.0	36.0	36.0	32.0	36.0
27	34.91149679895999	36.0	36.0	36.0	32.0	36.0
28	34.87496609994523	36.0	36.0	36.0	32.0	36.0
29	34.844692257489946	36.0	36.0	36.0	32.0	36.0
30	34.831951960779165	36.0	36.0	36.0	32.0	36.0
31	34.81754596847426	36.0	36.0	36.0	32.0	36.0
32	34.77299648489239	36.0	36.0	36.0	32.0	36.0
33	34.740788370925216	36.0	36.0	36.0	32.0	36.0
34	34.716305970083965	36.0	36.0	36.0	32.0	36.0
35	34.66205625921863	36.0	36.0	36.0	32.0	36.0
36	34.632404428090766	36.0	36.0	36.0	32.0	36.0
37	34.60099854251636	36.0	36.0	36.0	32.0	36.0
38	34.54687743314909	36.0	36.0	36.0	32.0	36.0
39	34.4888737833161	36.0	36.0	36.0	32.0	36.0
40	34.44435841849902	36.0	36.0	36.0	32.0	36.0
41	34.41864511760476	36.0	36.0	36.0	32.0	36.0
42	34.34244916741466	36.0	36.0	36.0	32.0	36.0
43	34.35272941369965	36.0	36.0	36.0	32.0	36.0
44	34.306520358404505	36.0	36.0	36.0	32.0	36.0
45	34.215873799063225	36.0	36.0	36.0	32.0	36.0
46	34.22792122239661	36.0	36.0	36.0	32.0	36.0
47	34.164852248251634	36.0	36.0	36.0	32.0	36.0
48	34.12616244381339	36.0	36.0	36.0	32.0	36.0
49	34.12816495285487	36.0	36.0	36.0	32.0	36.0
50	33.59917887506058	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	28.0
22	72.0
23	298.0
24	809.0
25	1975.0
26	4144.0
27	7979.0
28	13380.0
29	20280.0
30	28152.0
31	39201.0
32	56785.0
33	86009.0
34	183690.0
35	700260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13077678244876	18.13055278967253	11.890429033663665	25.848241394215037
2	15.566637358214896	20.85475693669812	37.01771192853987	26.56089377654711
3	18.96439653807358	24.37240991966322	28.659400225534377	28.003793316728824
4	12.357849042441229	15.852931990625189	35.702669056736156	36.08654991019743
5	14.061567748493964	36.91956544941412	33.49132945954127	15.527537342550648
6	34.239789897109425	35.00186779510069	16.89256635046835	13.865775957321539
7	29.672503313459863	30.826943349678277	20.846146107176757	18.654407229685102
8	28.196447099292605	33.08339151020151	19.845223285444586	18.8749381050613
9	27.23403548001603	14.374498060479448	19.03503384756436	39.356432611940164
10	16.09837052278696	27.02136184612262	31.535536880635064	25.34473075045535
11	37.2891854013679	21.069649521549938	22.166174131677437	19.474990945404727
12	24.718345222410605	23.93317621204725	28.933324934868153	22.415153630673995
13	30.075516199414558	19.47927766200727	25.35855322439824	25.086652914179936
14	23.419732543877604	20.297340660994205	25.101962616331864	31.180964178796327
15	25.15812735222638	27.831113863941365	23.10898933219954	23.901769451632717
16	25.303963200725065	25.78468784829572	25.184897459989187	23.726451490990023
17	23.457175701140617	26.29366983183823	26.382991008393216	23.866163458627938
18	24.858669578134595	24.082161484988617	28.247362794449316	22.81180614242747
19	25.211317631702805	25.76089219695101	25.736221705483324	23.291568465862863
20	25.134856604955445	24.253630149090256	26.80203942729466	23.80947381865964
21	25.996486642066163	24.753601279366197	24.639609611343527	24.610302467224116
22	25.042298519945476	25.390834824935745	25.371325890193567	24.195540764925212
23	23.21624473127536	24.993307473059296	26.203561299172577	25.586886496492767
24	24.204901554240962	25.945570946909452	25.92378742784756	23.925740071002025
25	24.698048931557757	24.793231536936624	25.748906887266354	24.759812644239265
26	23.257887121128615	25.935072865433845	26.673262961193846	24.1337770522437
27	24.780371387129	25.538770289729552	25.967266981959046	23.7135913411824
28	23.67422353564886	24.9123847616848	26.429707470959684	24.983684231706654
29	23.56915523688046	25.515587026470914	26.52725214467056	24.388005591978065
30	24.000538901515746	25.34997979119316	26.84000748863145	23.80947381865964
31	24.116192765772055	25.299763968134826	25.485842462290016	25.098200803803106
32	23.67483592373494	25.65206208565385	25.74689475498353	24.92620723562769
33	23.29996693104335	25.239312515637767	26.498644872649525	24.962075680669358
34	23.94069983710477	25.569914598107196	26.962572589859203	23.52681297492883
35	24.969161885665393	25.29617712363066	26.366456530069133	23.36820446063482
36	23.575804021815014	26.054488542218913	26.09429376781393	24.275413668152147
37	24.733392472525644	25.688980338843077	25.603333490804552	23.974293697826724
38	23.856277765238403	25.613219184194087	25.785300236381804	24.745202814185706
39	24.381356807043513	24.998906449846288	25.912152054212097	24.707584688898105
40	25.185422364062966	25.138093513410425	26.02588127019787	23.650602852328735
41	23.452626532501185	25.237912771441017	27.125292852731164	24.18416784332663
42	25.239399999650065	25.96499239763933	26.084495558436693	22.71111204427391
43	23.921103418350295	24.697873963533166	26.26978669648122	25.111235921635323
44	23.311164884617337	25.489954210867964	26.689097567419555	24.509783337095143
45	23.560056899601598	25.752056311709037	26.192625797635483	24.495260991053886
46	23.221689055303067	24.978282074947618	26.850791512293704	24.949237357455612
47	24.1958032169621	24.601116645932954	27.023199010380853	24.17988112672409
48	23.998701737257516	26.483772590559074	25.37071350210749	24.14681217007592
49	23.19866044480371	25.49327860333524	26.696183772415594	24.611877179445457
50	22.837263990005827	27.016812677483188	25.21927867682181	24.926644655689174
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	19.5
2	20.0
3	75.5
4	131.0
5	171.5
6	212.0
7	212.0
8	212.0
9	324.5
10	437.0
11	614.5
12	792.0
13	1423.0
14	2054.0
15	2866.0
16	3678.0
17	4503.0
18	5328.0
19	5774.5
20	6221.0
21	6811.0
22	7401.0
23	7582.5
24	7764.0
25	8334.5
26	8905.0
27	10801.0
28	12697.0
29	15058.0
30	17419.0
31	19757.0
32	22095.0
33	26132.0
34	30169.0
35	34916.0
36	39663.0
37	44529.0
38	49395.0
39	50646.0
40	51897.0
41	54720.0
42	57543.0
43	59425.0
44	61307.0
45	69908.0
46	78509.0
47	85817.0
48	93125.0
49	91906.0
50	90687.0
51	83244.5
52	75802.0
53	73009.0
54	70216.0
55	71370.5
56	72525.0
57	70307.5
58	68090.0
59	62124.5
60	56159.0
61	49431.5
62	42704.0
63	37276.0
64	31848.0
65	27357.5
66	22867.0
67	19842.5
68	16818.0
69	15596.5
70	14375.0
71	11094.5
72	7814.0
73	7189.0
74	6564.0
75	4715.5
76	2867.0
77	2598.0
78	2329.0
79	2031.5
80	1734.0
81	1383.0
82	1032.0
83	889.5
84	747.0
85	646.0
86	545.0
87	400.0
88	255.0
89	159.5
90	64.0
91	39.0
92	14.0
93	10.5
94	7.0
95	6.5
96	6.0
97	4.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015047250115041474
2	0.004461684627134391
3	2.624520368902583E-4
4	2.624520368902583E-4
5	0.0
6	6.123880860772694E-4
7	8.748401229675276E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.748401229675276E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1143066.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.95413445687687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.88402466929105	46.636337508850254
2	11.650812420069917	13.27123874209026
3	2.9536112245327506	5.0466111244613705
4	1.1408768235175726	2.599106080214176
5	0.6210971734849866	1.7687025964725052
6	0.34989777231803465	1.195687486245782
7	0.23414959482527517	0.9335051254691374
8	0.17064789352862805	0.7775282458249831
9	0.12373553633571852	0.6342525336202457
>10	0.6771270823215849	7.3892994976213435
>50	0.0868492398518836	3.448196799929311
>100	0.09653061604990736	11.273862471398024
>500	0.008481122652156399	3.2795441755229637
>1k	0.0021588312205489016	1.7461276122796736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2885	0.2523913754761317	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2585	0.22614617178710592	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1452	0.127026785854885	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1450	0.1268518178302915	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1269	0.11101721160457925	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1258	0.11005488746931498	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1230	0.10760533512500589	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1206	0.10550571882988384	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1160	0.1014814542642332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.748401229675277E-5	0.0
4	0.0	0.0	0.0	8.748401229675277E-5	0.0
5	0.0	0.0	0.0	8.748401229675277E-5	0.0
6	0.0	0.0	0.0	8.748401229675277E-5	0.0
7	0.0	0.0	0.0	8.748401229675277E-5	0.0
8	0.0	0.0	0.0	8.748401229675277E-5	0.0
9	0.0	0.0	0.0	8.748401229675277E-5	0.0
10	0.0	0.0	0.0	8.748401229675277E-5	0.0
11	0.0	0.0	0.0	8.748401229675277E-5	0.0
12	0.0	0.0	0.0	8.748401229675277E-5	8.748401229675277E-5
13	0.0	0.0	0.0	8.748401229675277E-5	8.748401229675277E-5
14	0.0	0.0	0.0	8.748401229675277E-5	8.748401229675277E-5
15	0.0	0.0	0.0	1.7496802459350555E-4	1.7496802459350555E-4
16	0.0	0.0	0.0	2.624520368902583E-4	1.7496802459350555E-4
17	0.0	0.0	0.0	2.624520368902583E-4	1.7496802459350555E-4
18	0.0	0.0	0.0	2.624520368902583E-4	2.624520368902583E-4
19	0.0	0.0	0.0	5.249040737805166E-4	2.624520368902583E-4
20	0.0	0.0	0.0	6.998720983740222E-4	2.624520368902583E-4
21	0.0	0.0	0.0	8.748401229675276E-4	2.624520368902583E-4
22	0.0	0.0	0.0	0.001487228209044797	2.624520368902583E-4
23	0.0	0.0	0.0	0.0027120043811993356	2.624520368902583E-4
24	0.0	0.0	0.0	0.0058614288238824355	2.624520368902583E-4
25	0.0	0.0	0.0	0.007173689008333727	2.624520368902583E-4
26	0.0	0.0	0.0	0.009973177401829816	3.499360491870111E-4
27	0.0	0.0	0.0	0.013035117832216163	3.499360491870111E-4
28	0.0	0.0	0.0	0.0220459710987817	4.374200614837638E-4
29	0.0	0.0	0.0	0.03814302936138421	4.374200614837638E-4
30	0.0	0.0	0.0	0.07689844680884568	4.374200614837638E-4
31	0.0	0.0	0.0	0.15178476133486604	5.249040737805166E-4
32	0.0	0.0	0.0	0.2511665993039772	5.249040737805166E-4
33	0.0	8.748401229675277E-5	0.0	0.3450369444983929	7.87356110670775E-4
34	0.0	8.748401229675277E-5	0.0	0.4612157128284806	7.87356110670775E-4
35	0.0	8.748401229675277E-5	0.0	0.5920917952244228	7.87356110670775E-4
36	0.0	8.748401229675277E-5	0.0	0.7779078373427256	7.87356110670775E-4
37	0.0	8.748401229675277E-5	0.0	1.0444716228109312	7.87356110670775E-4
38	0.0	8.748401229675277E-5	0.0	1.3725366689237541	7.87356110670775E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTACG	30	0.005745025	29.333185	2
TTAGGAC	590	0.0	21.254133	3
GACGTGA	490	0.0	21.101936	7
GATATAC	360	0.0	20.78222	1
TCTATAC	130	1.6243575E-9	20.30759	3
TAGGACG	845	0.0	20.047237	4
TATGTCG	55	0.0044835936	19.9999	16
TAGTACT	115	2.021261E-7	19.130339	4
CTAGCGG	370	0.0	19.026932	29
CGTCTTA	175	9.094947E-12	18.85705	15
TTTAGGA	690	0.0	18.811502	2
AGGACGT	870	0.0	18.712551	5
TGTAGGA	1000	0.0	18.699907	2
GGACGTG	885	0.0	18.643976	6
TAGCGGC	390	0.0	18.615294	30
CGCAATA	355	0.0	18.591455	36
TAGGGTG	120	3.1617674E-7	18.33324	5
CTGTAGG	950	0.0	18.298649	1
TCTAGCG	385	0.0	18.285624	28
CCGGTCC	445	0.0	17.797665	9
>>END_MODULE