FastQCFastQC Report
Thu 2 Feb 2017
SRR4062536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993208
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG102971.0367415485980782No Hit
TCCATGTACTCTGCGTTGATACCAC83500.8407101030197098No Hit
GAGTACATGGAAGCAGTGGTATCAA40170.40444700405151796No Hit
CATGTACTCTGCGTTGATACCACTG34160.34393601340303337No Hit
GCTTCCATGTACTCTGCGTTGATAC25460.2563410685375067No Hit
CCCATGTACTCTGCGTTGATACCAC24290.2445610587107635No Hit
GTACATGGGAAGCAGTGGTATCAAC23650.23811729265169027No Hit
CATGGAAGCAGTGGTATCAACGCAG22940.23096873967990592No Hit
GCGTTGATACCACTGCTTCCATGTA21760.21908804600848963No Hit
GTATCAACGCAGAGTACTTTTTTTT21050.2119394930367053No Hit
GTATCAACGCAGAGTACATGGAAGC17150.17267279361422785No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15700.15807363613664008No Hit
TATCAACGCAGAGTACTTTTTTTTT14640.14740114860130005No Hit
ACGCAGAGTACATGGAAGCAGTGGT14060.1415614856102649No Hit
ACTCTGCGTTGATACCACTGCTTCC13840.1393464410274585No Hit
CAGTGGTATCAACGCAGAGTACATG13010.13098968191959792No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11340.11417547985920372No Hit
TATCAACGCAGAGTACATGGAAGCA11220.1129672737231275No Hit
GTCTTGCGCCGGTCCAAGAATTTCA10930.11004744222760993No Hit
GAATAGGACCGCGGTTCTATTTTGT10830.10904060378087972No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10800.10873855224686067No Hit
GAATAACGCCGCCGCATCGCCAGTC10500.1057180369066701No Hit
GTATCTGATCGTCTTCGAACCTCCG10360.10430846308124783No Hit
GTCCTATTCCATTATTCCTAGCTGC10290.1036036761685367No Hit
GTATCAACGCAGAGTACATGGGAAG10220.10289888925582556No Hit
GATTAAGAGGGACGGCCGGGGGCAT10080.1014893154304033No Hit
GCGCAAGACGGACCAGAGCGAAAGC10000.10068384467301913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCC250.00602881819.0009578
GGGTAAA451.6768427E-618.9885141
TGCACCG350.002169455616.2857155
CGTCTTA551.1258726E-515.54780615
TCTATAC704.4515582E-714.9225543
CGCGCTA801.2828423E-714.253592516
TGTACCG602.5664132E-514.2500015
CGTACTA400.005291457814.2442563
CGCATCG1500.013.93473813
GTCGGCG759.662563E-713.93333212
CGGTTCT2400.013.85416712
TAGAAAT707.271341E-613.5686924
ACGCGCG852.6923772E-713.41311613
TAAGACT852.7023088E-713.4090614
CTATACT500.001501159713.2973194
ATTATAC655.469263E-513.1485433
GGTCGGC801.9973231E-613.062511
TATACTG801.9973231E-613.06255
GCGCCGA1051.981607E-812.67049919
CGAACGA751.4734824E-512.6698616
Produced by FastQC (version 0.11.5)