##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062536_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	993208
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07179362228254	32.0	32.0	32.0	32.0	32.0
2	30.69088247376179	32.0	32.0	32.0	32.0	32.0
3	30.699003632673115	32.0	32.0	32.0	32.0	32.0
4	30.76659974547124	32.0	32.0	32.0	32.0	32.0
5	30.534030132661034	32.0	32.0	32.0	32.0	32.0
6	34.19450306481623	36.0	36.0	36.0	32.0	36.0
7	34.04396561445337	36.0	36.0	36.0	32.0	36.0
8	34.01501397491764	36.0	36.0	36.0	32.0	36.0
9	34.21605846912228	36.0	36.0	36.0	32.0	36.0
10	33.83802486488228	36.0	36.0	36.0	32.0	36.0
11	34.222102520318	36.0	36.0	36.0	32.0	36.0
12	33.964289453971375	36.0	36.0	36.0	32.0	36.0
13	34.09117727605899	36.0	36.0	36.0	32.0	36.0
14	33.97812744158323	36.0	36.0	36.0	32.0	36.0
15	33.91942171226974	36.0	36.0	36.0	32.0	36.0
16	33.915517192773315	36.0	36.0	36.0	32.0	36.0
17	33.81292337556685	36.0	36.0	36.0	32.0	36.0
18	33.83716703852566	36.0	36.0	36.0	32.0	36.0
19	33.834239152322574	36.0	36.0	36.0	32.0	36.0
20	33.816117067119876	36.0	36.0	36.0	32.0	36.0
21	33.755107691440266	36.0	36.0	36.0	32.0	36.0
22	33.69905397459545	36.0	36.0	36.0	27.0	36.0
23	33.62585480584127	36.0	36.0	36.0	27.0	36.0
24	33.59735120941434	36.0	36.0	36.0	27.0	36.0
25	33.06483234126185	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	35.0
5	91.0
6	192.0
7	45.0
8	146.0
9	179.0
10	118.0
11	34.0
12	73.0
13	55.0
14	220.0
15	240.0
16	388.0
17	532.0
18	741.0
19	1125.0
20	1725.0
21	2554.0
22	3805.0
23	5612.0
24	7499.0
25	10337.0
26	13916.0
27	17477.0
28	23121.0
29	30257.0
30	38388.0
31	51610.0
32	73581.0
33	103520.0
34	219041.0
35	386545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.8562274326428	16.51271021751257	11.113158493582967	25.51790385626167
2	15.884447818570468	20.11057422680101	37.39366216965925	26.611315784969275
3	19.420077972709553	24.743950670984162	27.774757140785848	28.061214215520437
4	12.315961932151744	15.649869243728281	35.36481352252462	36.66935530159536
5	14.029017913349145	37.12258330941194	33.76845095106438	15.079947826174541
6	34.339832465036444	34.514297686824854	17.478755938062832	13.667113910075871
7	29.92044508837084	30.397714106085306	21.1668859733084	18.514954832235457
8	28.61060107990011	32.808667417773	19.859741976833945	18.72098952549294
9	27.447711901217748	14.648024727813649	17.97483076222256	39.92943260874604
10	15.552551517240962	27.220921816374634	32.02607263321096	25.200454033173454
11	37.396004600848194	21.072872636949327	21.499892640155004	20.03123012204748
12	24.16787483692222	23.78354562850731	29.219807674082375	22.828771860488096
13	30.667997318534873	19.638506242470978	24.733592407219795	24.959904031774354
14	23.96988982385325	19.63663747521314	24.65318557155199	31.740287129381617
15	25.595368893852676	27.08719309245076	23.40843525785375	23.90900275584281
16	26.06170873316668	25.29896782517539	24.83983146520442	23.799491976453513
17	24.090345474157484	25.75531518090265	26.24222506729034	23.912114277649525
18	25.50247608600971	23.79008517090639	27.658364654073846	23.04907408901005
19	24.92793979910856	26.825550293937063	24.858374862256383	23.388135044697993
20	25.04576742085462	23.70560862604993	27.095018811014825	24.15360514208062
21	26.01070055680399	25.087936316867136	23.49091803599315	25.410445090335728
22	24.851340082061686	25.71753556796307	24.388797742849853	25.042326607125393
23	23.343957040028958	25.211759949264323	24.958988996851204	26.48529401385552
24	23.690190772506657	26.617714964380124	25.014165277024425	24.67792898608879
25	25.247906055803565	24.7831452110478	24.400650060287848	25.56829867286079
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	204.0
1	204.0
2	422.0
3	640.0
4	640.0
5	640.0
6	1604.0
7	2568.0
8	2568.0
9	2568.0
10	2566.0
11	2564.0
12	2564.0
13	2564.0
14	2616.5
15	2669.0
16	2669.0
17	2669.0
18	4299.5
19	5930.0
20	5930.0
21	5930.0
22	9637.5
23	13345.0
24	13345.0
25	13345.0
26	20240.5
27	27136.0
28	27136.0
29	27136.0
30	34454.5
31	41773.0
32	41773.0
33	41773.0
34	50999.5
35	60226.0
36	60226.0
37	60226.0
38	68431.0
39	76636.0
40	76636.0
41	76636.0
42	91202.5
43	105769.0
44	105769.0
45	105769.0
46	135002.0
47	164235.0
48	164235.0
49	164235.0
50	150021.5
51	135808.0
52	135808.0
53	135808.0
54	120389.0
55	104970.0
56	104970.0
57	104970.0
58	96158.0
59	87346.0
60	87346.0
61	87346.0
62	77598.0
63	67850.0
64	67850.0
65	67850.0
66	56205.0
67	44560.0
68	44560.0
69	44560.0
70	33618.5
71	22677.0
72	22677.0
73	22677.0
74	17577.5
75	12478.0
76	12478.0
77	12478.0
78	10227.5
79	7977.0
80	7977.0
81	7977.0
82	5572.5
83	3168.0
84	3168.0
85	3168.0
86	2335.5
87	1503.0
88	1503.0
89	1503.0
90	1035.5
91	568.0
92	568.0
93	568.0
94	352.0
95	136.0
96	136.0
97	136.0
98	304.0
99	472.0
100	472.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0070478691271113406
2	0.0029198314955175553
3	0.004027353786920766
4	0.013995054409549661
5	0.03755507406303614
6	0.046314568549588805
7	0.06906911744569114
8	0.09001135713767912
9	0.09715991010946348
10	0.12182745205435318
11	0.1225322389670643
12	0.13592319030857586
13	0.12212950358837223
14	0.12595548968594694
15	0.11417547985920372
16	0.11357137679116559
17	0.1246465997051977
18	0.1329026749683853
19	0.13380882957044243
20	0.12444523201585167
21	0.13058694654090583
22	0.15253602467962402
23	0.14105806638689983
24	0.1355204549298838
25	0.13088899807492488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	993208.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.810834798589156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59392483284668	41.238276904859426
2	12.601497230563632	13.057881824552222
3	3.576403899924779	5.558894148960983
4	1.3966441474260716	2.894451967788344
5	0.7183759812152455	1.8609829643008735
6	0.4207633912577354	1.3080061532249199
7	0.2768910263780825	1.0042168657420638
8	0.21125203745007876	0.8756115530553122
9	0.1557404222227742	0.7262137158522011
>10	0.8463257160251506	8.195685032157808
>50	0.09165567133668391	3.3667379408938296
>100	0.09456947659611863	10.141318690476513
>500	0.010896894370597664	3.7738960901741363
>1k	0.004670097587398999	4.117884909121967
>5k	1.9458739947495832E-4	0.8418249232750258
>10k+	1.9458739947495832E-4	1.038116315564424
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	10297	1.0367415485980782	No Hit
TCCATGTACTCTGCGTTGATACCAC	8350	0.8407101030197098	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4017	0.40444700405151796	No Hit
CATGTACTCTGCGTTGATACCACTG	3416	0.34393601340303337	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2546	0.2563410685375067	No Hit
CCCATGTACTCTGCGTTGATACCAC	2429	0.2445610587107635	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2365	0.23811729265169027	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2294	0.23096873967990592	No Hit
GCGTTGATACCACTGCTTCCATGTA	2176	0.21908804600848963	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2105	0.2119394930367053	No Hit
GTATCAACGCAGAGTACATGGAAGC	1715	0.17267279361422785	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1570	0.15807363613664008	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1464	0.14740114860130005	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1406	0.1415614856102649	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1384	0.1393464410274585	No Hit
CAGTGGTATCAACGCAGAGTACATG	1301	0.13098968191959792	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1134	0.11417547985920372	No Hit
TATCAACGCAGAGTACATGGAAGCA	1122	0.1129672737231275	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1093	0.11004744222760993	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1083	0.10904060378087972	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1080	0.10873855224686067	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1050	0.1057180369066701	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1036	0.10430846308124783	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1029	0.1036036761685367	No Hit
GTATCAACGCAGAGTACATGGGAAG	1022	0.10289888925582556	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1008	0.1014893154304033	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1000	0.10068384467301913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.013676893460383E-4
13	0.0	0.0	0.0	1.0068384467301915E-4	2.013676893460383E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTCC	25	0.006028818	19.000957	8
GGGTAAA	45	1.6768427E-6	18.988514	1
TGCACCG	35	0.0021694556	16.285715	5
CGTCTTA	55	1.1258726E-5	15.547806	15
TCTATAC	70	4.4515582E-7	14.922554	3
CGCGCTA	80	1.2828423E-7	14.2535925	16
TGTACCG	60	2.5664132E-5	14.250001	5
CGTACTA	40	0.0052914578	14.244256	3
CGCATCG	150	0.0	13.934738	13
GTCGGCG	75	9.662563E-7	13.933332	12
CGGTTCT	240	0.0	13.854167	12
TAGAAAT	70	7.271341E-6	13.568692	4
ACGCGCG	85	2.6923772E-7	13.413116	13
TAAGACT	85	2.7023088E-7	13.409061	4
CTATACT	50	0.0015011597	13.297319	4
ATTATAC	65	5.469263E-5	13.148543	3
GGTCGGC	80	1.9973231E-6	13.0625	11
TATACTG	80	1.9973231E-6	13.0625	5
GCGCCGA	105	1.981607E-8	12.670499	19
CGAACGA	75	1.4734824E-5	12.66986	16
>>END_MODULE