FastQCFastQC Report
Thu 2 Feb 2017
SRR4062536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993208
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42010.4229728314713534No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC35980.3622604731335229No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA14400.14498473632914757No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC13950.1404539633188617No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11850.11931035593752769No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11630.11709531135472126No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11420.11498095061658786No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10200.10269752156647953No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10180.10249615387713348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT353.217474E-431.42853710
CCGTACG300.00574455829.33339
TCGTATA1006.002665E-1126.3999744
GTCGTAT1151.2732926E-1124.86953743
ATAACCG450.001398047824.4444165
ATTTCGT2000.023.09997442
TATACTA1053.0686351E-923.0475945
TGGTATA3800.020.84208144
AGTCGTA1401.8553692E-1020.42854942
TCCGTAC654.931349E-420.3076698
GATTTCG2200.019.99997741
GTGTAGG1553.45608E-1119.8719441
TGTAGGA5700.019.2982232
GTCCTAC5150.019.2242471
GTAGGAC5900.019.0169283
CTAAACC708.121992E-418.8571223
GAATACG600.007409090618.3342361
CGACAAT600.007411242518.33331320
TACGTGT600.007411242518.33331312
CGAACGA2050.018.24388116
Produced by FastQC (version 0.11.5)