##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	993208
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19542130147965	32.0	32.0	32.0	32.0	32.0
2	31.38445924720703	32.0	32.0	32.0	32.0	32.0
3	31.48554884777408	32.0	32.0	32.0	32.0	32.0
4	31.60080667896352	32.0	32.0	32.0	32.0	32.0
5	31.533388776570465	32.0	32.0	32.0	32.0	32.0
6	35.1220439223204	36.0	36.0	36.0	36.0	36.0
7	35.13524256751859	36.0	36.0	36.0	36.0	36.0
8	35.07638883295342	36.0	36.0	36.0	36.0	36.0
9	35.20469327673559	36.0	36.0	36.0	36.0	36.0
10	35.08727577707791	36.0	36.0	36.0	36.0	36.0
11	35.21122061038574	36.0	36.0	36.0	36.0	36.0
12	35.121591851857815	36.0	36.0	36.0	36.0	36.0
13	35.16276651013685	36.0	36.0	36.0	36.0	36.0
14	35.11887540172854	36.0	36.0	36.0	36.0	36.0
15	35.07763831946581	36.0	36.0	36.0	36.0	36.0
16	35.089069963189985	36.0	36.0	36.0	36.0	36.0
17	35.05613023656676	36.0	36.0	36.0	36.0	36.0
18	35.0689291669016	36.0	36.0	36.0	36.0	36.0
19	35.039282808837626	36.0	36.0	36.0	36.0	36.0
20	35.02676478642943	36.0	36.0	36.0	36.0	36.0
21	34.99182849916634	36.0	36.0	36.0	36.0	36.0
22	34.96469923721919	36.0	36.0	36.0	36.0	36.0
23	34.8995880017076	36.0	36.0	36.0	32.0	36.0
24	34.87837794298878	36.0	36.0	36.0	32.0	36.0
25	34.840598343952124	36.0	36.0	36.0	32.0	36.0
26	34.76739615468261	36.0	36.0	36.0	32.0	36.0
27	34.7457360391781	36.0	36.0	36.0	32.0	36.0
28	34.7055833219225	36.0	36.0	36.0	32.0	36.0
29	34.66104481639294	36.0	36.0	36.0	32.0	36.0
30	34.6388389944503	36.0	36.0	36.0	32.0	36.0
31	34.624912405055134	36.0	36.0	36.0	32.0	36.0
32	34.575066854072865	36.0	36.0	36.0	32.0	36.0
33	34.53872703401503	36.0	36.0	36.0	32.0	36.0
34	34.511116503290346	36.0	36.0	36.0	32.0	36.0
35	34.44009613293489	36.0	36.0	36.0	32.0	36.0
36	34.39254214625738	36.0	36.0	36.0	32.0	36.0
37	34.327160071203615	36.0	36.0	36.0	32.0	36.0
38	34.27163393770489	36.0	36.0	36.0	32.0	36.0
39	34.13585573213265	36.0	36.0	36.0	32.0	36.0
40	34.05547377789949	36.0	36.0	36.0	32.0	36.0
41	34.06779143945679	36.0	36.0	36.0	32.0	36.0
42	33.923541695193755	36.0	36.0	36.0	32.0	36.0
43	33.96306715209705	36.0	36.0	36.0	32.0	36.0
44	33.932449194931976	36.0	36.0	36.0	32.0	36.0
45	33.75459521067088	36.0	36.0	36.0	32.0	36.0
46	33.86805684207135	36.0	36.0	36.0	32.0	36.0
47	33.77328213224219	36.0	36.0	36.0	32.0	36.0
48	33.784212370419894	36.0	36.0	36.0	32.0	36.0
49	33.81749844946879	36.0	36.0	36.0	32.0	36.0
50	33.2320007490878	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	25.0
22	100.0
23	352.0
24	928.0
25	2323.0
26	4783.0
27	9009.0
28	14853.0
29	21240.0
30	29226.0
31	39809.0
32	57888.0
33	80710.0
34	168688.0
35	563266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.991163631420214	17.25694951437735	11.689282063934021	26.062604790268413
2	15.326534249320108	20.59077320869577	36.87977824608603	27.202914295898097
3	18.89124155130734	24.825790549949105	28.246951019984877	28.03601687875868
4	12.108364226555215	16.00030607950415	35.5152908862814	36.37603880765924
5	13.726429911962047	37.3055794959364	33.77046902562203	15.19752156647953
6	34.651154595476264	34.72545949728404	17.05055854531542	13.572827361924276
7	29.64504917398974	30.35909900041079	21.35997696353634	18.635874862063133
8	28.619684899839708	32.8122608758689	19.875494357677344	18.69255986661404
9	27.277569250348364	14.129870077566833	18.145745906194875	40.446814765889926
10	15.406541228020718	27.13691391934016	32.19023608347899	25.266308769160133
11	37.56705544055223	20.78346126893863	21.64581839856304	20.003664891946098
12	23.971816578199128	23.672986927209607	29.41327496355245	22.941921531038815
13	30.884265934225258	19.379525738818053	24.845148246892894	24.89106008006379
14	23.943826469380028	19.32576056576266	24.83961063543588	31.89080232942143
15	25.684549459931855	26.852582741983554	23.485312240739102	23.97755555734549
16	26.0978566423146	25.09504554937133	24.919050188882892	23.888047619431177
17	24.039375437974726	25.72784351314126	26.347653260948363	23.88512778793566
18	25.66400995561856	23.491856690642845	27.890935232096396	22.953198121642192
19	25.071888265096536	26.473810118323655	25.130184211162216	23.324117405417596
20	25.029500366489195	23.431144332305013	27.35177324387238	24.187582057333408
21	25.962839606608085	24.969392111219403	23.87475735193434	25.19301093023818
22	24.786046830069836	25.39689571570104	24.828334044832502	24.98872340939662
23	23.333279635282842	24.594344789812407	25.363569363114273	26.70880621179048
24	23.829650989520825	26.146688306981012	25.27849151436557	24.74516918913259
25	25.317254794564683	24.14801330637691	24.774468187932435	25.76026371112597
26	23.56364427189471	24.946134143099936	26.062315245145022	25.42790633986033
27	25.639644465207688	25.245869948691514	25.226941385892985	23.88754420020781
28	24.398212660389365	23.889960612479964	25.788555871479087	25.923270855651587
29	23.714569355059563	25.402534011002732	26.493443468034894	24.38945316590281
30	25.07712382501953	24.41341592093499	26.475119008304404	24.03434124574107
31	24.29944180876513	24.638242946089843	25.153643546971026	25.908671698173997
32	23.960741355285094	25.334270364314428	25.402332643313386	25.302655637087096
33	23.5666647872349	24.696639576000194	25.928204364040564	25.808491272724343
34	23.897008481607077	25.485296131323953	26.73719905598827	23.8804963310807
35	25.74435566366763	24.66311185572408	25.91571956730111	23.67681291330718
36	24.16231041232048	25.569165773936575	25.770835514816635	24.497688298926306
37	25.603297597280733	25.278692882054916	25.43354463516202	23.684464885502333
38	24.473322808515437	25.214456589153535	24.941402002400302	25.37081859993073
39	24.733691230839863	24.658782450403137	25.728850351587983	24.878675967169013
40	25.687771342961398	24.83759695854242	25.087896996399543	24.38673470209664
41	23.602407552093823	25.18767468647051	26.361446947668565	24.848470813767108
42	26.30486262696233	25.586584079065013	25.36789876843521	22.74065452553745
43	24.917640615057472	23.740042367761838	25.29319135568783	26.049125661492862
44	24.284943335132215	24.857431675943005	26.396182874080758	24.46144211484402
45	24.860754242817215	25.091118879429082	25.38078630055336	24.667340577200346
46	23.870955400032422	24.519662064403494	26.666747213823506	24.942635321740582
47	25.157066797689907	23.88925582556725	26.910475952670538	24.0432014240723
48	24.810915739704072	26.810798946444248	24.308100619407014	24.07018469444467
49	23.77075094038711	25.04671730392828	26.874028400898904	24.308503354785703
50	23.649326223711448	27.17970455332619	24.11962046217912	25.051348760783238
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	20.5
2	24.0
3	106.5
4	189.0
5	215.0
6	241.0
7	232.0
8	223.0
9	268.0
10	313.0
11	481.0
12	649.0
13	1122.0
14	1595.0
15	2172.5
16	2750.0
17	3118.5
18	3487.0
19	3745.5
20	4004.0
21	4282.5
22	4561.0
23	4902.0
24	5243.0
25	5709.0
26	6175.0
27	7581.5
28	8988.0
29	10529.5
30	12071.0
31	14158.5
32	16246.0
33	18934.0
34	21622.0
35	25414.5
36	29207.0
37	33329.0
38	37451.0
39	39561.5
40	41672.0
41	45446.5
42	49221.0
43	52122.5
44	55024.0
45	64653.5
46	74283.0
47	81193.5
48	88104.0
49	86331.0
50	84558.0
51	76614.5
52	68671.0
53	65896.0
54	63121.0
55	63944.5
56	64768.0
57	63050.5
58	61333.0
59	55831.0
60	50329.0
61	44616.0
62	38903.0
63	34041.0
64	29179.0
65	24849.0
66	20519.0
67	17764.5
68	15010.0
69	13890.0
70	12770.0
71	9932.0
72	7094.0
73	6283.5
74	5473.0
75	3941.0
76	2409.0
77	2256.0
78	2103.0
79	1789.0
80	1475.0
81	1154.5
82	834.0
83	738.0
84	642.0
85	511.5
86	381.0
87	284.0
88	187.0
89	120.0
90	53.0
91	37.0
92	21.0
93	13.0
94	5.0
95	4.5
96	4.0
97	3.0
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01540462823497193
2	0.0035239345635556703
3	7.04786912711134E-4
4	2.013676893460383E-4
5	0.0
6	3.0205153401905745E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0068384467301915E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	993208.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.36605672775883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.91811018096752	45.04668732035695
2	12.518500257645627	14.11236991337834
3	3.4269771669917413	5.79495568198172
4	1.4375291254335478	3.241113929279716
5	0.6925986533013825	1.9519527490777546
6	0.4421435585083261	1.4953133340416076
7	0.2734681653360151	1.0790025484396168
8	0.19696800259452812	0.8881847686237216
9	0.14245993134261284	0.7226914114341307
>10	0.7795510716249833	8.212815706427284
>50	0.07955301464991613	3.1450762452442036
>100	0.08242288928807452	9.48809004805528
>500	0.008098318596425312	3.1823079541652546
>1k	0.0016196637192850622	1.639438389494417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4201	0.4229728314713534	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3598	0.3622604731335229	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1440	0.14498473632914757	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1395	0.1404539633188617	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1185	0.11931035593752769	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1163	0.11709531135472126	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1142	0.11498095061658786	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1020	0.10269752156647953	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1018	0.10249615387713348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0068384467301915E-4
2	0.0	0.0	0.0	0.0	1.0068384467301915E-4
3	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
4	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
5	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
6	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
7	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
8	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
9	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
10	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
11	0.0	0.0	0.0	1.0068384467301915E-4	1.0068384467301915E-4
12	0.0	0.0	0.0	1.0068384467301915E-4	3.0205153401905745E-4
13	0.0	0.0	0.0	1.0068384467301915E-4	4.027353786920766E-4
14	0.0	0.0	0.0	1.0068384467301915E-4	4.027353786920766E-4
15	0.0	0.0	0.0	2.013676893460383E-4	4.027353786920766E-4
16	0.0	0.0	0.0	2.013676893460383E-4	4.027353786920766E-4
17	0.0	0.0	0.0	3.0205153401905745E-4	4.027353786920766E-4
18	0.0	0.0	0.0	6.041030680381149E-4	5.034192233650957E-4
19	0.0	0.0	0.0	0.0010068384467301914	5.034192233650957E-4
20	0.0	0.0	0.0	0.0013088899807492489	6.041030680381149E-4
21	0.0	0.0	0.0	0.0016109415147683064	6.041030680381149E-4
22	0.0	0.0	0.0	0.002718463806171517	6.041030680381149E-4
23	0.0	0.0	0.0	0.004430089165612843	6.041030680381149E-4
24	0.0	0.0	0.0	0.006645133748419263	6.041030680381149E-4
25	0.0	0.0	0.0	0.0077526560398224744	6.041030680381149E-4
26	0.0	0.0	0.0	0.010068384467301914	6.041030680381149E-4
27	0.0	0.0	0.0	0.012988215962819469	6.041030680381149E-4
28	0.0	0.0	0.0	0.018525827419835522	7.04786912711134E-4
29	0.0	0.0	0.0	0.025976431925638938	7.04786912711134E-4
30	0.0	0.0	0.0	0.039669434801169544	7.04786912711134E-4
31	0.0	0.0	0.0	0.06937116897971018	7.04786912711134E-4
32	1.0068384467301915E-4	0.0	0.0	0.105214617683305	7.04786912711134E-4
33	1.0068384467301915E-4	0.0	0.0	0.14015191178484265	7.04786912711134E-4
34	1.0068384467301915E-4	0.0	0.0	0.1866678480237775	7.04786912711134E-4
35	1.0068384467301915E-4	0.0	0.0	0.23247899735000122	7.04786912711134E-4
36	1.0068384467301915E-4	0.0	0.0	0.3082939323887846	7.04786912711134E-4
37	1.0068384467301915E-4	0.0	0.0	0.4254899275881789	7.04786912711134E-4
38	1.0068384467301915E-4	0.0	0.0	0.5515461011187989	7.04786912711134E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	35	3.217474E-4	31.428537	10
CCGTACG	30	0.005744558	29.3333	9
TCGTATA	100	6.002665E-11	26.39997	44
GTCGTAT	115	1.2732926E-11	24.869537	43
ATAACCG	45	0.0013980478	24.444416	5
ATTTCGT	200	0.0	23.099974	42
TATACTA	105	3.0686351E-9	23.047594	5
TGGTATA	380	0.0	20.842081	44
AGTCGTA	140	1.8553692E-10	20.428549	42
TCCGTAC	65	4.931349E-4	20.307669	8
GATTTCG	220	0.0	19.999977	41
GTGTAGG	155	3.45608E-11	19.871944	1
TGTAGGA	570	0.0	19.298223	2
GTCCTAC	515	0.0	19.224247	1
GTAGGAC	590	0.0	19.016928	3
CTAAACC	70	8.121992E-4	18.857122	3
GAATACG	60	0.0074090906	18.334236	1
CGACAAT	60	0.0074112425	18.333313	20
TACGTGT	60	0.0074112425	18.333313	12
CGAACGA	205	0.0	18.243881	16
>>END_MODULE