FastQCFastQC Report
Thu 2 Feb 2017
SRR4062535_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062535_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189018
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCA19940.16770141410811273No Hit
GCGCAAGACGGACCAGAGCGAAAGC19480.16383267536740403No Hit
GGGTAGGCACACGCTGAGCCAGTCA19390.16307574822248275No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC17680.1486941324689786No Hit
GAATAGGACCGCGGTTCTATTTTGT17550.1476007932596479No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17450.14675976309862424No Hit
GAACTACGACGGTATCTGATCGTCT16560.1392745946655139No Hit
GAATAACGCCGCCGCATCGCCAGTC16440.13826535847228555No Hit
GTATCTGATCGTCTTCGAACCTCCG16010.1346489287798839No Hit
GTGCATGGCCGTTCTTAGTTGGTGG15850.13330328052224608No Hit
GTCCTATTCCATTATTCCTAGCTGC15380.129350438765435No Hit
GTATCAACGCAGAGTACTTTTTTTT14720.12379963970267903No Hit
CTCTTAATCATGGCCTCAGTTCCGA14470.12169706430011994No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG14290.12018321001027739No Hit
GATTAAGAGGGACGGCCGGGGGCAT14260.1199309009619703No Hit
GTACATGGGGAATAATTGCAATCCC13370.11244573252885996No Hit
TCGTAGTTCCGACCATAAACGATGC13100.11017495109409614No Hit
CATCTAAGGGCATCACAGACCTGTT12680.10664262441779687No Hit
ATCAGATACCGTCGTAGTTCCGACC12510.1052128731440567No Hit
GGATTGACAGATTGATAGCTCTTTC12370.10403543091862362No Hit
GAATAATGGAATAGGACCGCGGTTC12200.10260567964488342No Hit
CTATTGGAGCTGGAATTACCGCGGC12050.10134413440334798No Hit
AGTCAGTGTAGCGCGCGTGCAGCCC11960.10058720725842671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGG402.7679524E-416.6190683
GGCGAAT400.005274409414.2515168
CCTAGTA400.005293597514.2437182
CGAACGA1500.013.93540216
GGTTAAG551.969694E-413.8097651
ATCGCCA1950.013.6430516
GGTCCAA4600.013.42534111
AATCGTA500.001498457713.30085513
CGGTCCA4700.013.13969710
CGCCAGT2000.012.82744618
TTACACA1600.012.4669264
TCGCCAG2150.012.37445217
CGACCAT2700.012.31612410
ACGGTAT3300.012.0921969
GTCCTAC3950.012.0179911
GATATAC2450.012.0130861
CGCCGGT5150.011.9915677
ACCTCCG4050.011.96524319
AGAATTT5400.011.96524317
GCCGGTC5200.011.8762648
Produced by FastQC (version 0.11.5)