##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062535_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1189018
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13605008502815	32.0	32.0	32.0	32.0	32.0
2	30.64112149689912	32.0	32.0	32.0	32.0	32.0
3	30.611134566507825	32.0	32.0	32.0	32.0	32.0
4	30.72399492690607	32.0	32.0	32.0	32.0	32.0
5	30.417364581528624	32.0	32.0	32.0	32.0	32.0
6	34.10944493691433	36.0	36.0	36.0	32.0	36.0
7	33.97406262983403	36.0	36.0	36.0	32.0	36.0
8	33.957820655364344	36.0	36.0	36.0	32.0	36.0
9	34.1900887959644	36.0	36.0	36.0	32.0	36.0
10	33.72774087524327	36.0	36.0	36.0	32.0	36.0
11	34.1793084713604	36.0	36.0	36.0	32.0	36.0
12	33.8588650466183	36.0	36.0	36.0	32.0	36.0
13	34.05469303240153	36.0	36.0	36.0	32.0	36.0
14	33.921410777633305	36.0	36.0	36.0	32.0	36.0
15	33.822648605824305	36.0	36.0	36.0	32.0	36.0
16	33.84453893885542	36.0	36.0	36.0	32.0	36.0
17	33.7347163793988	36.0	36.0	36.0	32.0	36.0
18	33.7665653505666	36.0	36.0	36.0	32.0	36.0
19	33.747637966792766	36.0	36.0	36.0	32.0	36.0
20	33.751118990629244	36.0	36.0	36.0	32.0	36.0
21	33.731023415961744	36.0	36.0	36.0	32.0	36.0
22	33.67680892972184	36.0	36.0	36.0	27.0	36.0
23	33.62783742550575	36.0	36.0	36.0	27.0	36.0
24	33.5975409960152	36.0	36.0	36.0	27.0	36.0
25	33.115771165785546	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	43.0
5	100.0
6	202.0
7	47.0
8	170.0
9	214.0
10	146.0
11	38.0
12	78.0
13	66.0
14	145.0
15	179.0
16	301.0
17	467.0
18	636.0
19	1071.0
20	1664.0
21	2585.0
22	3987.0
23	6191.0
24	8700.0
25	12246.0
26	17245.0
27	22263.0
28	29505.0
29	38828.0
30	49477.0
31	66715.0
32	94235.0
33	130998.0
34	268952.0
35	431515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.96578230439972	17.3042357035857	10.730942530050458	25.999039461964124
2	17.24792509832023	19.85589231706045	36.58950757461067	26.306675010008647
3	19.603301364298133	23.77189197645343	27.722561593590385	28.902245065658054
4	12.398915938098787	14.89937435968178	35.97044905127761	36.73126065094183
5	14.703078793385579	36.417258742317735	33.35344076929872	15.526221694997961
6	34.52971734071071	35.56648812198254	16.59994379460462	13.303850742702133
7	30.347185169351025	29.905355979674177	20.766813494220873	18.98064535675393
8	28.37751159521545	32.57813617959747	19.39132484280171	19.653027382385375
9	27.322356989884568	13.833206497582266	18.486843877537275	40.35759263499589
10	15.873186947537887	26.390027937805964	31.145264802911328	26.591520311744826
11	38.489181677263126	20.981323795258074	21.78761447921587	18.741880048262928
12	24.576034250739802	23.54106770820175	28.778638425301352	23.10425961575709
13	29.33880748575749	19.09556920351419	25.610663819568526	25.954959491159794
14	23.207012550680584	19.994526707561985	24.627879267589268	32.170581474168166
15	25.229202151709135	27.616659664378506	21.946237582164624	25.207900601747735
16	25.973342583417036	25.58487274539734	23.835663955909432	24.606120715276194
17	23.962397923869606	25.953819234519543	25.025892687231494	25.057890154379358
18	25.112798670163045	24.6529304075573	26.31118454182435	23.923086380455306
19	25.677119780916602	24.813283109879382	24.933704872365563	24.57589223683845
20	25.625739414531047	24.454969918196017	24.630198005212215	25.289092662060717
21	27.141916381470804	24.106348864156942	23.957378937724002	24.794355816648252
22	26.039270647073735	24.326138571665602	24.89061306376911	24.74397771749155
23	24.375205808364726	24.058629381560078	25.573547382454475	25.992617427620722
24	24.83254031748705	25.060676238999534	25.10084635142532	25.005937092088086
25	24.696510374562113	24.81852937213689	25.109471840474267	25.37548841282673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	82.0
2	246.5
3	411.0
4	411.0
5	411.0
6	1052.0
7	1693.0
8	1693.0
9	1693.0
10	1920.5
11	2148.0
12	2148.0
13	2148.0
14	2621.5
15	3095.0
16	3095.0
17	3095.0
18	5140.5
19	7186.0
20	7186.0
21	7186.0
22	12104.5
23	17023.0
24	17023.0
25	17023.0
26	25823.5
27	34624.0
28	34624.0
29	34624.0
30	43969.5
31	53315.0
32	53315.0
33	53315.0
34	65337.5
35	77360.0
36	77360.0
37	77360.0
38	86897.5
39	96435.0
40	96435.0
41	96435.0
42	109215.0
43	121995.0
44	121995.0
45	121995.0
46	136584.5
47	151174.0
48	151174.0
49	151174.0
50	152723.0
51	154272.0
52	154272.0
53	154272.0
54	142227.5
55	130183.0
56	130183.0
57	130183.0
58	121199.0
59	112215.0
60	112215.0
61	112215.0
62	101353.0
63	90491.0
64	90491.0
65	90491.0
66	76435.0
67	62379.0
68	62379.0
69	62379.0
70	47136.0
71	31893.0
72	31893.0
73	31893.0
74	25690.0
75	19487.0
76	19487.0
77	19487.0
78	16009.5
79	12532.0
80	12532.0
81	12532.0
82	8745.5
83	4959.0
84	4959.0
85	4959.0
86	3734.5
87	2510.0
88	2510.0
89	2510.0
90	1680.0
91	850.0
92	850.0
93	850.0
94	503.5
95	157.0
96	157.0
97	157.0
98	353.0
99	549.0
100	549.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00849440462633871
2	0.0038687387407087197
3	0.0046256658856299905
4	0.0131200705119687
5	0.03389351548925248
6	0.043733568373229
7	0.06526394049543405
8	0.08645790055322963
9	0.09503640819567072
10	0.11606216222126158
11	0.11412779285090721
12	0.12951864479763972
13	0.11572575015685212
14	0.12051962207468685
15	0.11025905411019848
16	0.10773596362712759
17	0.11976269492976557
18	0.12649093621795462
19	0.1282570995561043
20	0.11967859191366322
21	0.12867761463661612
22	0.1445730846799628
23	0.13683560719854534
24	0.1318735292485059
25	0.12598631812134048
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1189018.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.91055986081455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21177776361978	39.0359361589187
2	13.226350344488818	13.202691012174286
3	3.9336907869948536	5.88998128494724
4	1.6291702214233008	3.2525119143921657
5	0.7672790769833204	1.9147664150863275
6	0.46987686326173606	1.407109038662199
7	0.28151842301651697	0.983551947272159
8	0.2182451947684581	0.8714191886261007
9	0.1487930768288302	0.6683711191146098
>10	0.8762318289760478	8.276733108592802
>50	0.1067286627581462	3.7595699059803014
>100	0.10957150976680173	11.892100092255347
>500	0.015363646238446046	5.102729282073335
>1k	0.00540260087505795	3.742529531904438
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCA	1994	0.16770141410811273	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1948	0.16383267536740403	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1939	0.16307574822248275	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1768	0.1486941324689786	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1755	0.1476007932596479	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1745	0.14675976309862424	No Hit
GAACTACGACGGTATCTGATCGTCT	1656	0.1392745946655139	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1644	0.13826535847228555	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1601	0.1346489287798839	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1585	0.13330328052224608	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1538	0.129350438765435	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1472	0.12379963970267903	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1447	0.12169706430011994	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1429	0.12018321001027739	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1426	0.1199309009619703	No Hit
GTACATGGGGAATAATTGCAATCCC	1337	0.11244573252885996	No Hit
TCGTAGTTCCGACCATAAACGATGC	1310	0.11017495109409614	No Hit
CATCTAAGGGCATCACAGACCTGTT	1268	0.10664262441779687	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1251	0.1052128731440567	No Hit
GGATTGACAGATTGATAGCTCTTTC	1237	0.10403543091862362	No Hit
GAATAATGGAATAGGACCGCGGTTC	1220	0.10260567964488342	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1205	0.10134413440334798	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCC	1196	0.10058720725842671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.410301610236347E-5
2	0.0	0.0	0.0	0.0	8.410301610236347E-5
3	0.0	0.0	0.0	0.0	8.410301610236347E-5
4	0.0	0.0	0.0	0.0	8.410301610236347E-5
5	0.0	8.410301610236347E-5	0.0	0.0	8.410301610236347E-5
6	0.0	8.410301610236347E-5	0.0	0.0	8.410301610236347E-5
7	0.0	8.410301610236347E-5	0.0	0.0	8.410301610236347E-5
8	0.0	8.410301610236347E-5	0.0	0.0	8.410301610236347E-5
9	0.0	8.410301610236347E-5	0.0	0.0	8.410301610236347E-5
10	0.0	8.410301610236347E-5	0.0	0.0	8.410301610236347E-5
11	0.0	8.410301610236347E-5	0.0	0.0	8.410301610236347E-5
12	0.0	8.410301610236347E-5	0.0	0.0	1.6820603220472693E-4
13	0.0	8.410301610236347E-5	0.0	0.0	1.6820603220472693E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTAGG	40	2.7679524E-4	16.619068	3
GGCGAAT	40	0.0052744094	14.251516	8
CCTAGTA	40	0.0052935975	14.243718	2
CGAACGA	150	0.0	13.935402	16
GGTTAAG	55	1.969694E-4	13.809765	1
ATCGCCA	195	0.0	13.64305	16
GGTCCAA	460	0.0	13.425341	11
AATCGTA	50	0.0014984577	13.300855	13
CGGTCCA	470	0.0	13.139697	10
CGCCAGT	200	0.0	12.827446	18
TTACACA	160	0.0	12.466926	4
TCGCCAG	215	0.0	12.374452	17
CGACCAT	270	0.0	12.316124	10
ACGGTAT	330	0.0	12.092196	9
GTCCTAC	395	0.0	12.017991	1
GATATAC	245	0.0	12.013086	1
CGCCGGT	515	0.0	11.991567	7
ACCTCCG	405	0.0	11.965243	19
AGAATTT	540	0.0	11.965243	17
GCCGGTC	520	0.0	11.876264	8
>>END_MODULE