FastQCFastQC Report
Thu 2 Feb 2017
SRR4062535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189018
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20240.17022450459118363No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC20240.17022450459118363No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT19990.16812192918862456No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19650.1652624266411442No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA17370.14608693896980535No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16900.14213409721299425No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16570.13935869768161624No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16110.13548995894090754No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG15730.13229404432901773No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT15210.12792068749169483No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC14440.12144475525181285No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14130.11883756175263957No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT13210.11110008427122214No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12940.10882930283645832No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC12920.10866109680425359No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG12720.10697903648220633No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12720.10697903648220633No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT12690.10672672743389923No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12670.1065585214016945No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC12150.1021851645643716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC500.002579940522.0006943
GTATCAA19450.021.9487511
GCATATA1601.8189894E-1220.629991
AATCACG2250.020.5331234
CTACGCA808.986234E-519.2506084
TACGACG5800.018.5867925
TAGGACG3650.018.0827624
CGCAATA5100.017.68608936
TGTAGGA5600.017.6791292
ACCGTCG4000.017.5998158
CATATAG1651.6589183E-917.333882
GCGAGAA3300.017.3331520
ATCACGG2550.017.25471935
GATATAC3200.017.1916581
AACGCAG24600.017.1712726
GACGGTA6150.017.1705518
CGTCGTA4000.017.0498210
TACCGTC3900.016.92297
TACGAAT5600.016.8926841
TAGCGGC5350.016.85963630
Produced by FastQC (version 0.11.5)