##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062535_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1189018
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281874622587715	32.0	32.0	32.0	32.0	32.0
2	31.376943830959664	32.0	32.0	32.0	32.0	32.0
3	31.49023900395116	32.0	32.0	32.0	32.0	32.0
4	31.600749526079504	32.0	32.0	32.0	32.0	32.0
5	31.529119828295283	32.0	32.0	32.0	32.0	32.0
6	35.122644905291594	36.0	36.0	36.0	36.0	36.0
7	35.14156471979398	36.0	36.0	36.0	36.0	36.0
8	35.08171869559586	36.0	36.0	36.0	36.0	36.0
9	35.21562247165308	36.0	36.0	36.0	36.0	36.0
10	35.08637968474825	36.0	36.0	36.0	36.0	36.0
11	35.22133222541627	36.0	36.0	36.0	36.0	36.0
12	35.13426373696613	36.0	36.0	36.0	36.0	36.0
13	35.18991806684171	36.0	36.0	36.0	36.0	36.0
14	35.14419293904718	36.0	36.0	36.0	36.0	36.0
15	35.09940724194251	36.0	36.0	36.0	36.0	36.0
16	35.1133145166852	36.0	36.0	36.0	36.0	36.0
17	35.08370604986636	36.0	36.0	36.0	36.0	36.0
18	35.10358211566183	36.0	36.0	36.0	36.0	36.0
19	35.08989939597214	36.0	36.0	36.0	36.0	36.0
20	35.08919881784801	36.0	36.0	36.0	36.0	36.0
21	35.08367156762976	36.0	36.0	36.0	36.0	36.0
22	35.07170623152888	36.0	36.0	36.0	36.0	36.0
23	35.032500769542594	36.0	36.0	36.0	36.0	36.0
24	35.004490260029705	36.0	36.0	36.0	36.0	36.0
25	34.97211396295094	36.0	36.0	36.0	32.0	36.0
26	34.93476970071101	36.0	36.0	36.0	32.0	36.0
27	34.92010718088372	36.0	36.0	36.0	32.0	36.0
28	34.910267127999745	36.0	36.0	36.0	32.0	36.0
29	34.88074192316685	36.0	36.0	36.0	32.0	36.0
30	34.86945109325511	36.0	36.0	36.0	32.0	36.0
31	34.87775290197457	36.0	36.0	36.0	32.0	36.0
32	34.84354147708445	36.0	36.0	36.0	32.0	36.0
33	34.81685643110533	36.0	36.0	36.0	32.0	36.0
34	34.81529043294551	36.0	36.0	36.0	32.0	36.0
35	34.79106876430802	36.0	36.0	36.0	32.0	36.0
36	34.76189342802211	36.0	36.0	36.0	32.0	36.0
37	34.75052270024508	36.0	36.0	36.0	32.0	36.0
38	34.723960444669466	36.0	36.0	36.0	32.0	36.0
39	34.709503977231634	36.0	36.0	36.0	32.0	36.0
40	34.7091616779561	36.0	36.0	36.0	32.0	36.0
41	34.673218571964426	36.0	36.0	36.0	32.0	36.0
42	34.65208264298774	36.0	36.0	36.0	32.0	36.0
43	34.64891868752197	36.0	36.0	36.0	32.0	36.0
44	34.60737011550708	36.0	36.0	36.0	32.0	36.0
45	34.580604330632504	36.0	36.0	36.0	32.0	36.0
46	34.580807859931475	36.0	36.0	36.0	32.0	36.0
47	34.566740789458194	36.0	36.0	36.0	32.0	36.0
48	34.514236117535646	36.0	36.0	36.0	32.0	36.0
49	34.50354830624936	36.0	36.0	36.0	32.0	36.0
50	33.99693612712339	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	18.0
22	71.0
23	221.0
24	674.0
25	1537.0
26	3213.0
27	5907.0
28	10196.0
29	16432.0
30	24323.0
31	36543.0
32	55702.0
33	95878.0
34	217397.0
35	720899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.85418191300544	17.802592508474863	11.145347027699968	26.197878550819727
2	16.728134590783426	20.466589905354816	36.299409487395366	26.505866016466396
3	19.017433846169375	24.16146555661059	28.088853827010563	28.73224677020948
4	12.172996685497347	15.442420083144311	36.345905903784384	36.03867732757395
5	14.478081912973565	36.80347984639425	33.237427860637936	15.481010379994247
6	34.757487321385014	35.776065802642535	16.312226137711207	13.154220738261243
7	30.405763411487463	30.01989877360982	20.72163751936472	18.852700295538
8	28.442967221690502	32.789831608941164	19.18154308849824	19.585658080870097
9	27.447271614054625	13.591888432302959	18.443791431248307	40.51704852239411
10	15.891853613654291	26.503131155289488	31.11231284976342	26.492702381292798
11	38.91345631437035	20.802040002758577	21.69849405139367	18.586009631477403
12	24.57078025732159	23.637320881601458	28.679464902970352	23.112433958106607
13	29.570199946510485	19.014514498518945	25.60196733775267	25.813318217217905
14	23.371891762782397	19.921481424166835	24.513674309388083	32.19295250366269
15	25.283132803708607	27.7488650297977	21.746769182636427	25.221232983857266
16	26.19110896554972	25.57412923942278	23.731516259636106	24.50324553539139
17	24.174402742431152	25.961760040638577	24.785327051398717	25.078510165531558
18	25.36723581981097	24.549333988215487	26.20347210891677	23.879958083056774
19	25.77614468410066	24.803829714941237	24.82208006943545	24.59794553152265
20	25.77681750822948	24.30467831437371	24.65547199453667	25.26303218286014
21	27.290167179975413	24.082057630750754	23.88365861576528	24.74411657350856
22	26.13240506031027	24.379529998704815	24.807362041617537	24.680702899367375
23	24.525532834658517	23.992740227650046	25.458571695298136	26.023155242393305
24	25.01400315218104	25.027039119676907	24.957485925360256	25.001471802781793
25	24.983137345271476	24.62931595652883	25.08675226110959	25.3007944370901
26	24.529401573399227	25.65713891631582	25.62703003655117	24.186429473733785
27	25.485232351402587	24.695589133217496	24.805511775263284	25.013666740116637
28	24.489957258847216	24.70786817356844	25.61138687555613	25.19078769202821
29	24.509721467631273	24.631839047011905	25.35024701055829	25.50819247479853
30	24.234284089896033	25.236623835804	25.769836957892984	24.759255116406983
31	25.247725433929514	24.949496138830533	24.370362769949654	25.432415657290303
32	24.845124295847498	24.857571542230648	24.543951395184934	25.75335276673692
33	24.367166855337764	24.411825556888118	25.403231910702782	25.81777567707133
34	25.33670642496581	24.77229108390285	25.41870686566562	24.47229562546572
35	25.974880111150544	24.370278666933554	25.38498155620857	24.269859665707333
36	24.25699190424367	25.483886703144947	24.934273492916002	25.324847899695378
37	25.764622570894634	25.091714339059628	24.632007253044108	24.511655837001626
38	24.709045615793872	24.54807244297395	25.248902876154943	25.493979065077234
39	25.73342035192066	24.387099270154025	24.473893582771666	25.405586795153646
40	25.937622475017196	24.687599346687772	25.06656753724502	24.30821064105001
41	24.421581506755995	25.27791841671026	25.822233136924755	24.47826693960899
42	26.19918285509555	25.555962987944675	24.82813548659482	23.416718670364954
43	24.94024480705927	24.229826630042606	25.33569718877258	25.494231374125537
44	24.475575643093713	25.21500936066569	24.791634777606394	25.5177802186342
45	24.696850678459032	25.43990082572341	24.88036345959439	24.982885036223166
46	24.602760095103772	24.747753816808338	25.113265832195843	25.53622025589205
47	25.10819853021569	24.44445752713584	25.442087504142073	25.005256438506397
48	26.011464923155074	25.259920371264354	24.16792681019127	24.560687895389304
49	24.64277243910521	25.560168138749788	24.64394988133064	25.15310954081435
50	24.633689313366155	25.733504454936764	24.493069070443006	25.13973716125408
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	5.0
2	9.0
3	28.0
4	47.0
5	61.0
6	75.0
7	76.0
8	77.0
9	106.5
10	136.0
11	224.0
12	312.0
13	517.5
14	723.0
15	1067.0
16	1411.0
17	1731.5
18	2052.0
19	2307.5
20	2563.0
21	3240.0
22	3917.0
23	4549.0
24	5181.0
25	6615.0
26	8049.0
27	10584.0
28	13119.0
29	14890.0
30	16661.0
31	18218.0
32	19775.0
33	23066.0
34	26357.0
35	30899.0
36	35441.0
37	41627.5
38	47814.0
39	50579.0
40	53344.0
41	57726.0
42	62108.0
43	62070.5
44	62033.0
45	68782.5
46	75532.0
47	80319.0
48	85106.0
49	88643.0
50	92180.0
51	86517.0
52	80854.0
53	79108.5
54	77363.0
55	79966.5
56	82570.0
57	80909.5
58	79249.0
59	72796.0
60	66343.0
61	58466.0
62	50589.0
63	44425.5
64	38262.0
65	32954.5
66	27647.0
67	24810.0
68	21973.0
69	20601.5
70	19230.0
71	14766.0
72	10302.0
73	9349.5
74	8397.0
75	5966.5
76	3536.0
77	3251.0
78	2966.0
79	2572.5
80	2179.0
81	1785.5
82	1392.0
83	1214.5
84	1037.0
85	833.0
86	629.0
87	476.0
88	323.0
89	213.0
90	103.0
91	63.5
92	24.0
93	18.5
94	13.0
95	10.0
96	7.0
97	5.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017493427349291598
2	0.004289253821220537
3	7.569271449212712E-4
4	8.410301610236347E-5
5	0.0
6	6.728241288189077E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.410301610236347E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1189018.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.550293295843176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73916856281772	40.074769399882456
2	13.725126981975096	14.150686428870143
3	4.040624775045612	6.248861767561549
4	1.59506044191919	3.2890333442212594
5	0.7862904080385478	2.0266750575047676
6	0.46117555834226676	1.4264241176050851
7	0.28748928904221666	1.0374110018677791
8	0.21744195942728153	0.8967357426639356
9	0.13648942377314466	0.6332462844557614
>10	0.7967087872392657	7.710227063880809
>50	0.09748442333101315	3.529746477248976
>100	0.09941486127650924	11.071734320365847
>500	0.012447328137171773	4.301508911791794
>1k	0.005077199634899012	3.6029400820798267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2024	0.17022450459118363	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2024	0.17022450459118363	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1999	0.16812192918862456	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1965	0.1652624266411442	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1737	0.14608693896980535	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1690	0.14213409721299425	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1657	0.13935869768161624	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1611	0.13548995894090754	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1573	0.13229404432901773	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	1521	0.12792068749169483	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1444	0.12144475525181285	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1413	0.11883756175263957	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1321	0.11110008427122214	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1294	0.10882930283645832	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1292	0.10866109680425359	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1272	0.10697903648220633	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1272	0.10697903648220633	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	1269	0.10672672743389923	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1267	0.1065585214016945	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	1215	0.1021851645643716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.410301610236347E-5
2	0.0	0.0	0.0	0.0	8.410301610236347E-5
3	0.0	0.0	0.0	0.0	8.410301610236347E-5
4	0.0	0.0	0.0	0.0	8.410301610236347E-5
5	0.0	0.0	0.0	0.0	8.410301610236347E-5
6	0.0	0.0	0.0	0.0	8.410301610236347E-5
7	0.0	0.0	0.0	0.0	8.410301610236347E-5
8	0.0	0.0	0.0	0.0	8.410301610236347E-5
9	0.0	0.0	0.0	0.0	8.410301610236347E-5
10	0.0	0.0	0.0	0.0	8.410301610236347E-5
11	0.0	0.0	0.0	0.0	8.410301610236347E-5
12	0.0	0.0	0.0	0.0	2.523090483070904E-4
13	0.0	0.0	0.0	0.0	3.3641206440945386E-4
14	0.0	0.0	0.0	0.0	3.3641206440945386E-4
15	0.0	0.0	0.0	0.0	4.205150805118173E-4
16	0.0	0.0	0.0	0.0	4.205150805118173E-4
17	0.0	0.0	0.0	0.0	4.205150805118173E-4
18	0.0	0.0	0.0	0.0	4.205150805118173E-4
19	0.0	0.0	0.0	0.0	4.205150805118173E-4
20	0.0	8.410301610236347E-5	0.0	1.6820603220472693E-4	4.205150805118173E-4
21	0.0	8.410301610236347E-5	0.0	1.6820603220472693E-4	4.205150805118173E-4
22	0.0	8.410301610236347E-5	0.0	2.523090483070904E-4	4.205150805118173E-4
23	0.0	8.410301610236347E-5	0.0	3.3641206440945386E-4	4.205150805118173E-4
24	0.0	8.410301610236347E-5	0.0	5.046180966141808E-4	4.205150805118173E-4
25	0.0	8.410301610236347E-5	0.0	5.887211127165443E-4	4.205150805118173E-4
26	0.0	8.410301610236347E-5	0.0	7.569271449212712E-4	4.205150805118173E-4
27	0.0	8.410301610236347E-5	0.0	0.0011774422254330885	4.205150805118173E-4
28	0.0	8.410301610236347E-5	0.0	0.0020184723864567233	4.205150805118173E-4
29	0.0	8.410301610236347E-5	0.0	0.003616429692401629	4.205150805118173E-4
30	0.0	8.410301610236347E-5	0.0	0.008326198594133983	4.205150805118173E-4
31	0.0	8.410301610236347E-5	0.0	0.018586766558622327	4.205150805118173E-4
32	0.0	8.410301610236347E-5	0.0	0.03162273405448866	4.205150805118173E-4
33	0.0	8.410301610236347E-5	0.0	0.044658701550355	4.205150805118173E-4
34	0.0	8.410301610236347E-5	0.0	0.06274085001236314	5.046180966141808E-4
35	0.0	8.410301610236347E-5	0.0	0.07939324720063111	5.046180966141808E-4
36	0.0	8.410301610236347E-5	0.0	0.10891340585256068	5.046180966141808E-4
37	0.0	8.410301610236347E-5	0.0	0.15416082851563223	5.046180966141808E-4
38	0.0	8.410301610236347E-5	0.0	0.22001349012378282	5.046180966141808E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGTAC	50	0.0025799405	22.000694	3
GTATCAA	1945	0.0	21.948751	1
GCATATA	160	1.8189894E-12	20.62999	1
AATCACG	225	0.0	20.53312	34
CTACGCA	80	8.986234E-5	19.250608	4
TACGACG	580	0.0	18.586792	5
TAGGACG	365	0.0	18.082762	4
CGCAATA	510	0.0	17.686089	36
TGTAGGA	560	0.0	17.679129	2
ACCGTCG	400	0.0	17.599815	8
CATATAG	165	1.6589183E-9	17.33388	2
GCGAGAA	330	0.0	17.33315	20
ATCACGG	255	0.0	17.254719	35
GATATAC	320	0.0	17.191658	1
AACGCAG	2460	0.0	17.171272	6
GACGGTA	615	0.0	17.170551	8
CGTCGTA	400	0.0	17.04982	10
TACCGTC	390	0.0	16.9229	7
TACGAAT	560	0.0	16.89268	41
TAGCGGC	535	0.0	16.859636	30
>>END_MODULE