FastQCFastQC Report
Thu 2 Feb 2017
SRR4062534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017269
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32310.16016703771286825No Hit
GTCTTGCGCCGGTCCAAGAATTTCA30040.14891420033718855No Hit
GCGCAAGACGGACCAGAGCGAAAGC29940.14841848062900884No Hit
GGGTAGGCACACGCTGAGCCAGTCA29400.14574159420483832No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG24710.12249233989120936No Hit
GAATAGGACCGCGGTTCTATTTTGT24600.12194704821221165No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC23530.11664284733468863No Hit
GAACTACGACGGTATCTGATCGTCT23440.11619669959732688No Hit
GAATAACGCCGCCGCATCGCCAGTC22510.11158650631125547No Hit
GTATCTGATCGTCTTCGAACCTCCG21560.10687716908354811No Hit
GATTAAGAGGGACGGCCGGGGGCAT21460.10638144937536838No Hit
GTGCATGGCCGTTCTTAGTTGGTGG21420.10618316149209649No Hit
GTCCTATTCCATTATTCCTAGCTGC20750.10286183944729235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCG1850.013.3502355
CGCCAGT4350.013.10918718
TCGCCAG4350.012.89005917
ACCGTAT751.4796142E-512.6662378
AACCGCG1650.012.6659227
CGGTCCA5050.012.60447110
CAAGACG5250.012.4840524
CGTTATT1750.012.4787852
ATCGCCA4500.012.45915416
GCATCGC4450.012.38375414
CGCATCG4450.012.38344713
GCGTTAT1850.012.3162621
ACTCTAA3650.012.23337210
ACGCTTA951.0354033E-612.00465817
TCGACGC951.0385138E-612.001677514
TCGCGTA2550.011.9217559
GTATCAA25000.011.8862191
AAGACGG5200.011.8740075
TTTCGTT2250.011.82681217
TCCAACG1052.7058013E-711.767055518
Produced by FastQC (version 0.11.5)