##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062534_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2017269
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238400530618375	32.0	32.0	32.0	32.0	32.0
2	30.85473033095735	32.0	32.0	32.0	32.0	32.0
3	30.84280728053621	32.0	32.0	32.0	32.0	32.0
4	30.929748090115893	32.0	32.0	32.0	32.0	32.0
5	30.691431831847908	32.0	32.0	32.0	32.0	32.0
6	34.37522511871248	36.0	36.0	36.0	32.0	36.0
7	34.268975035059775	36.0	36.0	36.0	32.0	36.0
8	34.255473613087794	36.0	36.0	36.0	32.0	36.0
9	34.45523279245356	36.0	36.0	36.0	32.0	36.0
10	34.07970875475705	36.0	36.0	36.0	32.0	36.0
11	34.41809248047732	36.0	36.0	36.0	32.0	36.0
12	34.17816513315775	36.0	36.0	36.0	32.0	36.0
13	34.32214146948176	36.0	36.0	36.0	32.0	36.0
14	34.2154031019165	36.0	36.0	36.0	32.0	36.0
15	34.13567005689375	36.0	36.0	36.0	32.0	36.0
16	34.14711870355416	36.0	36.0	36.0	32.0	36.0
17	34.05920777050557	36.0	36.0	36.0	32.0	36.0
18	34.08096788281583	36.0	36.0	36.0	32.0	36.0
19	34.06820210889078	36.0	36.0	36.0	32.0	36.0
20	34.071919015262715	36.0	36.0	36.0	32.0	36.0
21	34.05568072478187	36.0	36.0	36.0	32.0	36.0
22	33.999473545669915	36.0	36.0	36.0	32.0	36.0
23	33.96411980752195	36.0	36.0	36.0	32.0	36.0
24	33.93800826761329	36.0	36.0	36.0	32.0	36.0
25	33.48571162299128	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	77.0
5	214.0
6	342.0
7	88.0
8	299.0
9	401.0
10	270.0
11	85.0
12	161.0
13	137.0
14	298.0
15	371.0
16	576.0
17	810.0
18	1032.0
19	1593.0
20	2361.0
21	3514.0
22	5307.0
23	8202.0
24	11455.0
25	16683.0
26	23382.0
27	30454.0
28	40875.0
29	54519.0
30	71001.0
31	96304.0
32	139589.0
33	202922.0
34	455417.0
35	848525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.849867036307714	17.142252311743835	10.885522326200292	26.122358325748156
2	17.645440376286942	19.32550525881625	36.33144026524105	26.69761409965576
3	19.666226116338997	22.852267578305092	27.631081899727043	29.850424405628868
4	12.896401412424058	14.661336527582627	35.23102612944841	37.21123593054491
5	15.146804090787594	35.97187447155536	33.118360193818	15.76296124383905
6	35.20407910228802	34.70411034723552	16.38008933079274	13.711721219683723
7	31.283356805147893	29.72503996987935	20.095411810051424	18.89619141492133
8	28.625858940498226	32.3933137476624	18.988309626931173	19.9925176849082
9	27.286057600458907	14.274074335050097	18.451320868567166	39.988547195923836
10	16.1474143767285	26.146590455618444	30.896594922660608	26.80940024499245
11	38.20308036842123	21.09041329508889	21.609482821518604	19.09702351497127
12	24.993819837081972	23.45245245742141	28.065584186725307	23.488143518771317
13	29.429186325963975	19.02684120714455	25.25167044052378	26.292302026367697
14	23.47308361439743	19.704516631797343	24.394824946082846	32.42757480772237
15	25.39838679898912	27.194427845683805	21.67006803396338	25.737117321363694
16	26.236516660429782	25.66829818342801	23.314080045898198	24.781105110244013
17	24.419142559614514	25.781235264511633	24.734723982897652	25.064898192976205
18	25.31951320121774	24.517596742496707	25.862165675791456	24.300724380494096
19	25.739288678503492	24.769322870542823	25.04139976887611	24.449988682077574
20	25.94422643764537	24.16521815956377	24.541151664898013	25.349403737892846
21	26.980869739600323	23.910418022423787	24.119545885274032	24.989166352701858
22	26.20891366700378	24.095161068504073	24.677393809674882	25.01853145481726
23	24.521702954922073	24.01150951133228	25.413610652453862	26.053176881291783
24	24.8789144620089	24.782760854511203	25.01974450045545	25.318580183024448
25	24.958241190938896	24.522361128691593	25.05915727869072	25.460240401678792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	198.0
1	198.0
2	464.0
3	730.0
4	730.0
5	730.0
6	1833.0
7	2936.0
8	2936.0
9	2936.0
10	3435.0
11	3934.0
12	3934.0
13	3934.0
14	4832.5
15	5731.0
16	5731.0
17	5731.0
18	9126.0
19	12521.0
20	12521.0
21	12521.0
22	20366.0
23	28211.0
24	28211.0
25	28211.0
26	41638.0
27	55065.0
28	55065.0
29	55065.0
30	68259.5
31	81454.0
32	81454.0
33	81454.0
34	101969.0
35	122484.0
36	122484.0
37	122484.0
38	139816.5
39	157149.0
40	157149.0
41	157149.0
42	178837.0
43	200525.0
44	200525.0
45	200525.0
46	225475.0
47	250425.0
48	250425.0
49	250425.0
50	257783.5
51	265142.0
52	265142.0
53	265142.0
54	248243.0
55	231344.0
56	231344.0
57	231344.0
58	215071.0
59	198798.0
60	198798.0
61	198798.0
62	179140.0
63	159482.0
64	159482.0
65	159482.0
66	134838.5
67	110195.0
68	110195.0
69	110195.0
70	84184.5
71	58174.0
72	58174.0
73	58174.0
74	46831.5
75	35489.0
76	35489.0
77	35489.0
78	28493.0
79	21497.0
80	21497.0
81	21497.0
82	15311.0
83	9125.0
84	9125.0
85	9125.0
86	6640.0
87	4155.0
88	4155.0
89	4155.0
90	2759.0
91	1363.0
92	1363.0
93	1363.0
94	808.0
95	253.0
96	253.0
97	253.0
98	571.0
99	889.0
100	889.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008774238834781083
2	0.004213617519527639
3	0.004709337227707361
4	0.01541688292438936
5	0.0365841144636635
6	0.0467463684813478
7	0.06821103184552978
8	0.09170814601324861
9	0.10122596441029927
10	0.12457436266556418
11	0.12442564675311026
12	0.13676906748678536
13	0.12546665814028768
14	0.12908541200999965
15	0.11862572616740752
16	0.11773343069268402
17	0.12764782485627846
18	0.13483576062488445
19	0.13706649931169318
20	0.12645809755664714
21	0.13443918485834067
22	0.15496198077698117
23	0.14549373435074847
24	0.13800836675723466
25	0.1350340485081563
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2017269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.37088497080812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.03722236609326	37.74729506511672
2	13.007525920258612	12.583710800872685
3	3.862478764463188	5.604945480541139
4	1.5838232537489383	3.0644372968472373
5	0.8528702205395952	2.0627043666374254
6	0.5093718164929317	1.478325932577071
7	0.35201635549415916	1.191913984761575
8	0.24799095501047572	0.9596433566890057
9	0.19597794217299916	0.8531663847899246
>10	1.1201865497632975	9.963022303343834
>50	0.10662665829633648	3.623166216404414
>100	0.10149376555661745	10.513487987539913
>500	0.015937577876118002	5.34258840018758
>1k	0.0064778542335189315	5.011592423691572
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3231	0.16016703771286825	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	3004	0.14891420033718855	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2994	0.14841848062900884	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2940	0.14574159420483832	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2471	0.12249233989120936	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2460	0.12194704821221165	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2353	0.11664284733468863	No Hit
GAACTACGACGGTATCTGATCGTCT	2344	0.11619669959732688	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2251	0.11158650631125547	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2156	0.10687716908354811	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2146	0.10638144937536838	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2142	0.10618316149209649	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2075	0.10286183944729235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.957197081797222E-5
2	0.0	0.0	0.0	0.0	4.957197081797222E-5
3	0.0	0.0	0.0	0.0	4.957197081797222E-5
4	0.0	0.0	0.0	4.957197081797222E-5	4.957197081797222E-5
5	0.0	0.0	0.0	4.957197081797222E-5	4.957197081797222E-5
6	0.0	0.0	0.0	4.957197081797222E-5	4.957197081797222E-5
7	0.0	0.0	0.0	4.957197081797222E-5	9.914394163594444E-5
8	0.0	4.957197081797222E-5	0.0	4.957197081797222E-5	9.914394163594444E-5
9	0.0	4.957197081797222E-5	0.0	9.914394163594444E-5	9.914394163594444E-5
10	0.0	4.957197081797222E-5	0.0	9.914394163594444E-5	9.914394163594444E-5
11	0.0	4.957197081797222E-5	0.0	9.914394163594444E-5	9.914394163594444E-5
12	0.0	4.957197081797222E-5	0.0	9.914394163594444E-5	9.914394163594444E-5
13	0.0	4.957197081797222E-5	0.0	9.914394163594444E-5	2.478598540898611E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAACCG	185	0.0	13.350235	5
CGCCAGT	435	0.0	13.109187	18
TCGCCAG	435	0.0	12.890059	17
ACCGTAT	75	1.4796142E-5	12.666237	8
AACCGCG	165	0.0	12.665922	7
CGGTCCA	505	0.0	12.604471	10
CAAGACG	525	0.0	12.484052	4
CGTTATT	175	0.0	12.478785	2
ATCGCCA	450	0.0	12.459154	16
GCATCGC	445	0.0	12.383754	14
CGCATCG	445	0.0	12.383447	13
GCGTTAT	185	0.0	12.316262	1
ACTCTAA	365	0.0	12.233372	10
ACGCTTA	95	1.0354033E-6	12.004658	17
TCGACGC	95	1.0385138E-6	12.0016775	14
TCGCGTA	255	0.0	11.921755	9
GTATCAA	2500	0.0	11.886219	1
AAGACGG	520	0.0	11.874007	5
TTTCGTT	225	0.0	11.826812	17
TCCAACG	105	2.7058013E-7	11.7670555	18
>>END_MODULE