FastQCFastQC Report
Thu 2 Feb 2017
SRR4062534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017269
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC30440.1508970791699074No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT28640.14197412442267243No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC28550.14152797668531067No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24570.12179833229975774No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23220.11510611623933148No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23000.1140155328813361No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA22890.11347024120233842No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG22800.11302409346497666No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA21430.10623273346291445No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT20530.10177125608929696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30100.023.4680081
ATTTCGT2700.020.3702142
ATACGAA8350.020.28726440
CGGTCCA8800.020.2498410
GGTATCA14600.019.4435021
CGCAATA8700.019.21823936
CGAATGC8950.018.92722543
TAGCGGC8800.018.74985330
CTAGCGG8800.018.74985329
TAGGACG3450.018.4926094
GCCGGTC9400.018.4892168
CGCCGGT9450.018.391397
GTATACG600.007402463418.3381881
TACGAAT9250.018.31336841
CAATACG9300.018.2149138
GATATAC2900.018.2117181
TAGTACG1456.3082553E-918.2081072
TCTAGCG9100.018.13172728
AACGCAG38500.018.0570016
GATTTCG3050.018.03264441
Produced by FastQC (version 0.11.5)