##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062534_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2017269
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27181650042706	32.0	32.0	32.0	32.0	32.0
2	31.412800176872793	32.0	32.0	32.0	32.0	32.0
3	31.504691739178067	32.0	32.0	32.0	32.0	32.0
4	31.611176298252737	32.0	32.0	32.0	32.0	32.0
5	31.53559738438453	32.0	32.0	32.0	32.0	32.0
6	35.14159638600504	36.0	36.0	36.0	36.0	36.0
7	35.16032219798153	36.0	36.0	36.0	36.0	36.0
8	35.09361864976857	36.0	36.0	36.0	36.0	36.0
9	35.22341938531748	36.0	36.0	36.0	36.0	36.0
10	35.10395440568412	36.0	36.0	36.0	36.0	36.0
11	35.23634378954914	36.0	36.0	36.0	36.0	36.0
12	35.147820642660946	36.0	36.0	36.0	36.0	36.0
13	35.1933311819098	36.0	36.0	36.0	36.0	36.0
14	35.14946841497093	36.0	36.0	36.0	36.0	36.0
15	35.10989560638665	36.0	36.0	36.0	36.0	36.0
16	35.1266489496443	36.0	36.0	36.0	36.0	36.0
17	35.09479449691638	36.0	36.0	36.0	36.0	36.0
18	35.114820085967715	36.0	36.0	36.0	36.0	36.0
19	35.10000351960993	36.0	36.0	36.0	36.0	36.0
20	35.09757945023693	36.0	36.0	36.0	36.0	36.0
21	35.094762770855056	36.0	36.0	36.0	36.0	36.0
22	35.07769910706009	36.0	36.0	36.0	36.0	36.0
23	35.04155767029583	36.0	36.0	36.0	36.0	36.0
24	35.01820282768436	36.0	36.0	36.0	36.0	36.0
25	34.985294970576554	36.0	36.0	36.0	36.0	36.0
26	34.95115177995597	36.0	36.0	36.0	32.0	36.0
27	34.93120104458057	36.0	36.0	36.0	32.0	36.0
28	34.917528103589554	36.0	36.0	36.0	32.0	36.0
29	34.8971014772943	36.0	36.0	36.0	32.0	36.0
30	34.88726937260227	36.0	36.0	36.0	32.0	36.0
31	34.887925705495896	36.0	36.0	36.0	32.0	36.0
32	34.85719901510409	36.0	36.0	36.0	32.0	36.0
33	34.84000497702587	36.0	36.0	36.0	32.0	36.0
34	34.82886119798599	36.0	36.0	36.0	32.0	36.0
35	34.807636462960566	36.0	36.0	36.0	32.0	36.0
36	34.783190541271395	36.0	36.0	36.0	32.0	36.0
37	34.78128251611461	36.0	36.0	36.0	32.0	36.0
38	34.742197991442886	36.0	36.0	36.0	32.0	36.0
39	34.743960770725174	36.0	36.0	36.0	32.0	36.0
40	34.73991222786847	36.0	36.0	36.0	32.0	36.0
41	34.70950279808989	36.0	36.0	36.0	32.0	36.0
42	34.68834548094478	36.0	36.0	36.0	32.0	36.0
43	34.681435148212756	36.0	36.0	36.0	32.0	36.0
44	34.64224106948553	36.0	36.0	36.0	32.0	36.0
45	34.61126899783817	36.0	36.0	36.0	32.0	36.0
46	34.61575476547748	36.0	36.0	36.0	32.0	36.0
47	34.59650894352711	36.0	36.0	36.0	32.0	36.0
48	34.555931311094355	36.0	36.0	36.0	32.0	36.0
49	34.54028490994508	36.0	36.0	36.0	32.0	36.0
50	34.0470586718975	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	14.0
21	53.0
22	169.0
23	486.0
24	1302.0
25	2981.0
26	6004.0
27	10840.0
28	18321.0
29	28721.0
30	41401.0
31	60319.0
32	89742.0
33	151793.0
34	346996.0
35	1258126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16563128110495	17.75392892875523	11.485392387164458	26.595047402975364
2	16.971828053365485	19.83220172279772	36.03493781429954	27.161032409537256
3	19.024043964520242	23.153203127207508	28.091230543563235	29.73152236470901
4	12.633677148339808	15.132305242687261	35.433683295270285	36.80033431370265
5	14.857817602817274	36.15473997505537	33.06506621827145	15.92237620385591
6	35.42715224401476	34.91769756426431	16.071939343315545	13.583210848405383
7	31.177067201779835	29.77165156042727	20.08964599646651	18.961635241326388
8	28.61522186679119	32.47112804489634	18.935848416844753	19.977801671467713
9	27.322632727712566	14.015632025277739	18.3357797100932	40.325955536916496
10	16.025577154063242	26.198390001531774	30.88388311127569	26.892149733129294
11	38.46517246832227	20.90707783642142	21.539764899971196	19.08798479528511
12	24.896977051647546	23.469205148148315	28.03577509989991	23.598042700304223
13	29.502659288374527	18.950720008090148	25.29424682578278	26.252373877752543
14	23.567605510221988	19.501960323585994	24.46713849268491	32.463295673507105
15	25.492237277229762	27.176990277449363	21.60599305298401	25.724779392336867
16	26.369710732678687	25.55603640367249	23.29451352298578	24.779739340663046
17	24.455984799250867	25.7113453882452	24.736413438168135	25.096256374335795
18	25.485545061169333	24.353023815861942	25.841917959379735	24.31951316358899
19	25.847271732228077	24.626115803098152	25.037364873004048	24.489247591669727
20	26.001837137238514	23.965024000269672	24.571437919285927	25.46170094320589
21	27.112546715386	23.83802061103403	24.08503774161998	24.96439493195999
22	26.217376066355058	24.02371721371815	24.719211964294303	25.039694755632492
23	24.56201924483051	23.79246396985231	25.51246264132349	26.13305414399369
24	25.022939429496017	24.63023027667604	25.003655932847824	25.343174360980118
25	25.08227707856513	24.19488922895261	25.169275887350672	25.553557805131593
26	24.686841467350167	25.23401688123894	25.472606776785845	24.606534874625048
27	25.748573938329493	24.307913322417583	24.675390342091212	25.268122397161708
28	24.649513773324234	24.49747653882551	25.593066665873515	25.259943021976746
29	24.615953549080466	24.652141087777586	25.33137623192544	25.40052913121651
30	24.52687271752057	24.85196570214483	25.612796310259068	25.008365270075533
31	25.326419034843646	24.63900451551082	24.538422986721155	25.496153462924383
32	25.17140748209584	24.43253725705397	24.597909351702725	25.79814590914747
33	24.652041943835947	24.16603834193655	25.21939314984764	25.96252656437986
34	25.411583680708922	24.51948649386869	25.243881703431718	24.825048121990672
35	25.904229926697926	24.118895397688657	25.308771413232446	24.66810326238097
36	24.51190198233354	24.82752672053157	25.136409670698356	25.52416162643653
37	25.92445529079166	24.509671243646732	24.659279451575372	24.906594013986236
38	24.79332206066717	24.208422376985915	25.398744540267064	25.599511022079852
39	25.57338659345878	24.24664236648657	24.58204632104097	25.597924719013676
40	25.669556216845645	24.43833717763967	25.29494083337423	24.597165772140453
41	24.56598500249595	24.884435343030603	25.738411684311806	24.81116797016164
42	25.985825390664306	24.91556654070429	25.021006122634116	24.077601945997287
43	24.987247610507076	23.798908326058648	25.446978067872948	25.76686599556133
44	24.500599572987046	24.577783131550625	25.06686019564074	25.85475709982159
45	24.57575068074709	24.925728794721973	25.154999159755093	25.343521364775846
46	24.494203785818918	24.26384238064905	25.32037188966249	25.921581943869548
47	24.879874721715346	24.190526895520627	25.625189302963562	25.30440907980046
48	25.68422952020777	24.861235660687793	24.625074791710972	24.82946002739347
49	24.563308116071777	24.990965508318425	24.979910958826014	25.465815416783784
50	24.400525859727114	25.277008310249304	24.951072440548305	25.371393389475273
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	24.5
2	37.0
3	120.5
4	204.0
5	241.5
6	279.0
7	248.0
8	217.0
9	292.5
10	368.0
11	619.0
12	870.0
13	1468.5
14	2067.0
15	2818.5
16	3570.0
17	4009.0
18	4448.0
19	4854.5
20	5261.0
21	6306.0
22	7351.0
23	8085.5
24	8820.0
25	10922.5
26	13025.0
27	16504.0
28	19983.0
29	22896.0
30	25809.0
31	28482.5
32	31156.0
33	35744.0
34	40332.0
35	47097.5
36	53863.0
37	64057.5
38	74252.0
39	80309.0
40	86366.0
41	93899.5
42	101433.0
43	103451.0
44	105469.0
45	115905.5
46	126342.0
47	134359.0
48	142376.0
49	147393.0
50	152410.0
51	146199.5
52	139989.0
53	137618.5
54	135248.0
55	137768.5
56	140289.0
57	138443.5
58	136598.0
59	125749.5
60	114901.0
61	102054.0
62	89207.0
63	79240.0
64	69273.0
65	60179.0
66	51085.0
67	46620.0
68	42155.0
69	39209.0
70	36263.0
71	27692.5
72	19122.0
73	17062.5
74	15003.0
75	10913.0
76	6823.0
77	6103.5
78	5384.0
79	4697.0
80	4010.0
81	3150.0
82	2290.0
83	1938.5
84	1587.0
85	1314.0
86	1041.0
87	764.0
88	487.0
89	307.5
90	128.0
91	78.5
92	29.0
93	23.0
94	17.0
95	11.5
96	6.0
97	8.0
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015218595041117472
2	0.004213617519527639
3	2.974318249078333E-4
4	9.914394163594444E-5
5	4.957197081797222E-5
6	6.940075914516111E-4
7	4.957197081797222E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.9828788327188888E-4
47	0.0
48	0.0
49	0.0
50	4.957197081797222E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2017269.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.127744944733664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70368443268364	40.239419038850556
2	12.714007387686848	13.000770538862255
3	3.7656908289656745	5.77593840732239
4	1.5565929543445742	3.183403502099954
5	0.8000012181126791	2.0451129117570654
6	0.498122528288086	1.5280728946523487
7	0.3345698410251014	1.1974061048692035
8	0.22097332683093757	0.903829431504116
9	0.19529211589865697	0.8986360942245512
>10	1.0092385205145389	9.403788970562301
>50	0.09499467732753164	3.38610830508803
>100	0.0880384542724344	9.51432205523275
>500	0.01334061567234892	4.6309945742167695
>1k	0.005453098377018536	4.292197170757777
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3044	0.1508970791699074	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2864	0.14197412442267243	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2855	0.14152797668531067	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2457	0.12179833229975774	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2322	0.11510611623933148	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2300	0.1140155328813361	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2289	0.11347024120233842	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2280	0.11302409346497666	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	2143	0.10623273346291445	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2053	0.10177125608929696	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.957197081797222E-5
6	0.0	0.0	0.0	0.0	1.4871591245391665E-4
7	0.0	0.0	0.0	0.0	1.9828788327188888E-4
8	0.0	0.0	0.0	0.0	1.9828788327188888E-4
9	0.0	0.0	0.0	0.0	1.9828788327188888E-4
10	0.0	0.0	0.0	0.0	1.9828788327188888E-4
11	0.0	0.0	0.0	0.0	1.9828788327188888E-4
12	0.0	0.0	0.0	0.0	2.974318249078333E-4
13	0.0	0.0	0.0	0.0	2.974318249078333E-4
14	0.0	0.0	0.0	9.914394163594444E-5	3.4700379572580553E-4
15	0.0	0.0	0.0	9.914394163594444E-5	5.452916789976944E-4
16	0.0	0.0	0.0	1.4871591245391665E-4	5.452916789976944E-4
17	0.0	0.0	0.0	1.9828788327188888E-4	5.452916789976944E-4
18	0.0	0.0	0.0	2.974318249078333E-4	5.452916789976944E-4
19	0.0	0.0	0.0	3.4700379572580553E-4	6.444356206336388E-4
20	0.0	0.0	0.0	5.948636498156666E-4	6.444356206336388E-4
21	0.0	0.0	0.0	8.427235039055277E-4	6.940075914516111E-4
22	0.0	0.0	0.0	9.418674455414722E-4	6.940075914516111E-4
23	0.0	0.0	0.0	0.0014375871537211943	6.940075914516111E-4
24	0.0	0.0	0.0	0.0018341629202649722	6.940075914516111E-4
25	0.0	0.0	0.0	0.002280310657626722	6.940075914516111E-4
26	0.0	0.0	0.0	0.003023890219896305	6.940075914516111E-4
27	0.0	0.0	0.0	0.004362333431981555	6.940075914516111E-4
28	0.0	0.0	0.0	0.008278519126601361	6.940075914516111E-4
29	0.0	0.0	0.0	0.018044197377741888	6.940075914516111E-4
30	0.0	0.0	0.0	0.03940971680028792	6.940075914516111E-4
31	0.0	0.0	0.0	0.08486721404036844	6.940075914516111E-4
32	4.957197081797222E-5	0.0	0.0	0.13319988558789136	7.435795622695833E-4
33	4.957197081797222E-5	0.0	0.0	0.18480430720940044	7.435795622695833E-4
34	4.957197081797222E-5	0.0	0.0	0.2416137857667966	7.435795622695833E-4
35	4.957197081797222E-5	0.0	0.0	0.30536334023870887	7.435795622695833E-4
36	4.957197081797222E-5	0.0	0.0	0.3982116415807708	7.435795622695833E-4
37	4.957197081797222E-5	0.0	0.0	0.5319072468768419	7.435795622695833E-4
38	4.957197081797222E-5	0.0	0.0	0.7167115540862423	7.435795622695833E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3010	0.0	23.468008	1
ATTTCGT	270	0.0	20.37021	42
ATACGAA	835	0.0	20.287264	40
CGGTCCA	880	0.0	20.24984	10
GGTATCA	1460	0.0	19.443502	1
CGCAATA	870	0.0	19.218239	36
CGAATGC	895	0.0	18.927225	43
TAGCGGC	880	0.0	18.749853	30
CTAGCGG	880	0.0	18.749853	29
TAGGACG	345	0.0	18.492609	4
GCCGGTC	940	0.0	18.489216	8
CGCCGGT	945	0.0	18.39139	7
GTATACG	60	0.0074024634	18.338188	1
TACGAAT	925	0.0	18.313368	41
CAATACG	930	0.0	18.21491	38
GATATAC	290	0.0	18.211718	1
TAGTACG	145	6.3082553E-9	18.208107	2
TCTAGCG	910	0.0	18.131727	28
AACGCAG	3850	0.0	18.057001	6
GATTTCG	305	0.0	18.032644	41
>>END_MODULE