FastQCFastQC Report
Thu 2 Feb 2017
SRR4062533_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062533_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318677
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG21810.16539304166221144No Hit
TCCATGTACTCTGCGTTGATACCAC19020.144235472371172No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13460.10207200095246978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT706.548362E-1118.9889952
GCGTTAT855.820766E-1116.753091
TCGCGTA602.564239E-514.2520229
ATAACGA759.649884E-713.93583912
TAAGACT802.0023763E-613.0603864
GAACCGC1102.743036E-912.9549086
TAATAGT604.0968784E-412.6646184
CGACCAT2050.012.51397110
TAACGAA853.935771E-612.29586213
CCGACCA1950.012.1812169
AACCGCG1103.8098733E-812.0912487
CCGTCGT1900.012.0017029
AAGACGG1900.011.9989695
CTTATAC1353.7471182E-1011.9573963
ACGAACG802.8580893E-511.87803815
AAACGAT2000.011.87758717
CCGATAA802.8614239E-511.8766859
GTTATTC1459.822543E-1111.7876163
GTGTAAC1052.7430178E-711.7537541
CGTCGTA1950.011.69396710
Produced by FastQC (version 0.11.5)