##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062533_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1318677
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.158913062106944	32.0	32.0	32.0	32.0	32.0
2	30.69111541340298	32.0	32.0	32.0	32.0	32.0
3	30.687705177234456	32.0	32.0	32.0	32.0	32.0
4	30.7871798780141	32.0	32.0	32.0	32.0	32.0
5	30.496899544012674	32.0	32.0	32.0	32.0	32.0
6	34.190554624066394	36.0	36.0	36.0	32.0	36.0
7	34.07547185550366	36.0	36.0	36.0	32.0	36.0
8	34.049429845216075	36.0	36.0	36.0	32.0	36.0
9	34.28243838331904	36.0	36.0	36.0	32.0	36.0
10	33.82568968746706	36.0	36.0	36.0	32.0	36.0
11	34.254102407185385	36.0	36.0	36.0	32.0	36.0
12	33.975691545389815	36.0	36.0	36.0	32.0	36.0
13	34.12907558105586	36.0	36.0	36.0	32.0	36.0
14	33.99581474462662	36.0	36.0	36.0	32.0	36.0
15	33.920714473673236	36.0	36.0	36.0	32.0	36.0
16	33.93741151168937	36.0	36.0	36.0	32.0	36.0
17	33.84447745733034	36.0	36.0	36.0	32.0	36.0
18	33.852509750302765	36.0	36.0	36.0	32.0	36.0
19	33.84694432374266	36.0	36.0	36.0	32.0	36.0
20	33.856050420231796	36.0	36.0	36.0	32.0	36.0
21	33.82958374188676	36.0	36.0	36.0	32.0	36.0
22	33.781406667440166	36.0	36.0	36.0	32.0	36.0
23	33.73977554776492	36.0	36.0	36.0	32.0	36.0
24	33.698890630533484	36.0	36.0	36.0	27.0	36.0
25	33.213795341846414	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	58.0
5	128.0
6	205.0
7	57.0
8	180.0
9	215.0
10	148.0
11	44.0
12	91.0
13	89.0
14	156.0
15	220.0
16	376.0
17	534.0
18	731.0
19	1079.0
20	1731.0
21	2626.0
22	4087.0
23	6240.0
24	9086.0
25	12762.0
26	17792.0
27	23113.0
28	30891.0
29	41157.0
30	52724.0
31	71149.0
32	100384.0
33	141686.0
34	298175.0
35	500757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.240058366216154	16.90724276508432	10.830209370598928	26.022489498100594
2	17.1466316001814	19.064747110275906	36.87752059321816	26.911100696324542
3	19.58498785086136	22.607332558781508	28.060187530524523	29.747492059832613
4	12.619256915365229	14.58956053082458	35.442711552799175	37.34847100101101
5	14.754102091440796	36.28972182396092	33.715664186067684	15.240511898530606
6	35.57336752422065	34.54893064965215	16.288417331138238	13.589284494988963
7	30.956774853472798	29.879712621524245	20.294359575184924	18.869152949818037
8	28.48941699352755	33.1739001080766	18.904221666322602	19.43246123207325
9	26.87474714813457	14.270598745627039	18.223930545719792	40.630723560518604
10	15.450443713420878	26.905530683377126	32.00460985181209	25.639415751389905
11	38.19083192881762	20.886742890113727	21.463733126831563	19.45869205423708
12	24.110164440290262	23.612978763307577	28.855220107926044	23.42163668847612
13	29.529899183308117	19.07180921130052	25.12749185168201	26.270799753709355
14	22.96826468192175	19.505008302248932	24.934458926455058	32.59226808937426
15	25.370698215364808	27.066132015025314	21.9985059715588	25.564663798051086
16	26.074823326852727	25.790521710546795	23.38864752141372	24.746007441186762
17	24.219652169819845	26.037078906750022	24.993755395224643	24.749513528205494
18	25.068392156029024	24.87227010326969	25.862143584424736	24.197194156276552
19	25.427670692427895	25.449917920635862	24.846737098505866	24.27567428843038
20	25.331271263200172	24.59375472146489	25.088621184202236	24.986352831132706
21	26.237596323295488	24.55231354338373	24.273584354112867	24.93650577920791
22	25.311676002770454	24.637744996779958	24.843250665273338	25.207328335176253
23	24.30097728774176	24.369015346530894	25.374475097007387	25.95553226871996
24	24.46656405825141	25.32253692346359	24.98213717585903	25.228761842425975
25	24.87940513874958	24.654198553848747	24.882138596961457	25.584257710440216
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	88.0
2	254.0
3	420.0
4	420.0
5	420.0
6	1012.5
7	1605.0
8	1605.0
9	1605.0
10	1880.5
11	2156.0
12	2156.0
13	2156.0
14	2683.5
15	3211.0
16	3211.0
17	3211.0
18	5290.5
19	7370.0
20	7370.0
21	7370.0
22	11963.5
23	16557.0
24	16557.0
25	16557.0
26	24828.5
27	33100.0
28	33100.0
29	33100.0
30	42180.5
31	51261.0
32	51261.0
33	51261.0
34	64757.0
35	78253.0
36	78253.0
37	78253.0
38	91584.5
39	104916.0
40	104916.0
41	104916.0
42	121004.5
43	137093.0
44	137093.0
45	137093.0
46	158162.0
47	179231.0
48	179231.0
49	179231.0
50	180189.0
51	181147.0
52	181147.0
53	181147.0
54	170749.0
55	160351.0
56	160351.0
57	160351.0
58	146858.5
59	133366.0
60	133366.0
61	133366.0
62	116533.0
63	99700.0
64	99700.0
65	99700.0
66	81480.0
67	63260.0
68	63260.0
69	63260.0
70	47480.5
71	31701.0
72	31701.0
73	31701.0
74	24142.5
75	16584.0
76	16584.0
77	16584.0
78	13360.5
79	10137.0
80	10137.0
81	10137.0
82	7049.5
83	3962.0
84	3962.0
85	3962.0
86	2897.5
87	1833.0
88	1833.0
89	1833.0
90	1256.5
91	680.0
92	680.0
93	680.0
94	403.0
95	126.0
96	126.0
97	126.0
98	347.5
99	569.0
100	569.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008038359658961216
2	0.002957509685844221
3	0.004929182809740368
4	0.015166716337662672
5	0.033897611014676074
6	0.04451431245103994
7	0.06316937354636502
8	0.0828861047853265
9	0.09145529951610591
10	0.11359870536909343
11	0.11352287178740511
12	0.12618707992935344
13	0.1163287143098727
14	0.1186037217605221
15	0.10866952255935305
16	0.10722868450727509
17	0.11678371580000257
18	0.12436707396883391
19	0.12580791202091188
20	0.11784538594363896
21	0.12345707098857416
22	0.14597964475000322
23	0.1339979388432497
24	0.12861375454337945
25	0.12618707992935344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1318677.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.79821677229506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.54557714801304	48.76280099118794
2	11.57488250487063	13.843146662801992
3	3.0570530300030083	5.48418959317564
4	1.284766854981953	3.073070675842824
5	0.6712069382638972	2.0068488996686495
6	0.3907323029899993	1.401905697248043
7	0.2688578661345184	1.1254054672034033
8	0.1871253124944746	0.8951808000102436
9	0.1399615600451093	0.7532506536629434
>10	0.7394989285215027	8.048944848908016
>50	0.06622133947912107	2.7768866501361495
>100	0.06636137103746759	8.126012740024587
>500	0.006229300247519996	2.5030549915114424
>1k	0.0015255429177599988	1.1993013286182506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2181	0.16539304166221144	No Hit
TCCATGTACTCTGCGTTGATACCAC	1902	0.144235472371172	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1346	0.10207200095246978	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.583358168831337E-5	0.0
8	0.0	0.0	0.0	7.583358168831337E-5	0.0
9	0.0	0.0	0.0	7.583358168831337E-5	0.0
10	0.0	0.0	0.0	1.5166716337662673E-4	0.0
11	0.0	0.0	0.0	2.2750074506494008E-4	0.0
12	0.0	0.0	0.0	2.2750074506494008E-4	1.5166716337662673E-4
13	0.0	0.0	0.0	2.2750074506494008E-4	2.2750074506494008E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	70	6.548362E-11	18.988995	2
GCGTTAT	85	5.820766E-11	16.75309	1
TCGCGTA	60	2.564239E-5	14.252022	9
ATAACGA	75	9.649884E-7	13.935839	12
TAAGACT	80	2.0023763E-6	13.060386	4
GAACCGC	110	2.743036E-9	12.954908	6
TAATAGT	60	4.0968784E-4	12.664618	4
CGACCAT	205	0.0	12.513971	10
TAACGAA	85	3.935771E-6	12.295862	13
CCGACCA	195	0.0	12.181216	9
AACCGCG	110	3.8098733E-8	12.091248	7
CCGTCGT	190	0.0	12.001702	9
AAGACGG	190	0.0	11.998969	5
CTTATAC	135	3.7471182E-10	11.957396	3
ACGAACG	80	2.8580893E-5	11.878038	15
AAACGAT	200	0.0	11.877587	17
CCGATAA	80	2.8614239E-5	11.876685	9
GTTATTC	145	9.822543E-11	11.787616	3
GTGTAAC	105	2.7430178E-7	11.753754	1
CGTCGTA	195	0.0	11.693967	10
>>END_MODULE