FastQCFastQC Report
Thu 2 Feb 2017
SRR4062533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318677
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16200.12285040233506765No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC14000.1061670143636387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA1850.020.21600715
TAACGCC2850.019.2980464
TAGCGGC3500.018.85694730
ATAACGC3150.018.1585433
CTAGACA1701.2732926E-1018.117464
TACCGTC2800.018.0712417
AACGCCG3050.018.0325985
ATACCGT3050.018.0325986
TTAACGG1601.1023076E-917.87481535
AATAACG3150.017.4621242
CGCAAGA5350.017.2728142
TAGGACG902.2157384E-417.1109334
AAGACGG5500.016.7998265
CGCAATA3950.016.70868936
CTAGCGG4000.016.4998329
CGCCGGT3900.016.3588057
TAACGGC1753.6270649E-916.34268836
TACGAAT4050.016.29612741
CGAATGC4050.016.29612743
ACCGTCG2850.016.2103588
Produced by FastQC (version 0.11.5)