##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1318677
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308325693099977	32.0	32.0	32.0	32.0	32.0
2	31.396335872999984	32.0	32.0	32.0	32.0	32.0
3	31.498660399779475	32.0	32.0	32.0	32.0	32.0
4	31.608396142497366	32.0	32.0	32.0	32.0	32.0
5	31.53568993771788	32.0	32.0	32.0	32.0	32.0
6	35.139139455681715	36.0	36.0	36.0	36.0	36.0
7	35.162542457326545	36.0	36.0	36.0	36.0	36.0
8	35.09195580115525	36.0	36.0	36.0	36.0	36.0
9	35.23561948832049	36.0	36.0	36.0	36.0	36.0
10	35.116084530176835	36.0	36.0	36.0	36.0	36.0
11	35.24748441051145	36.0	36.0	36.0	36.0	36.0
12	35.16571760939184	36.0	36.0	36.0	36.0	36.0
13	35.2065138013327	36.0	36.0	36.0	36.0	36.0
14	35.163693611096576	36.0	36.0	36.0	36.0	36.0
15	35.12732230864723	36.0	36.0	36.0	36.0	36.0
16	35.14289397631111	36.0	36.0	36.0	36.0	36.0
17	35.114720284042264	36.0	36.0	36.0	36.0	36.0
18	35.13424970633446	36.0	36.0	36.0	36.0	36.0
19	35.11680115752379	36.0	36.0	36.0	36.0	36.0
20	35.11301175344683	36.0	36.0	36.0	36.0	36.0
21	35.11086717975668	36.0	36.0	36.0	36.0	36.0
22	35.0952727620183	36.0	36.0	36.0	36.0	36.0
23	35.0523729465214	36.0	36.0	36.0	36.0	36.0
24	35.03748378109272	36.0	36.0	36.0	36.0	36.0
25	35.011607088013214	36.0	36.0	36.0	36.0	36.0
26	34.96574217947231	36.0	36.0	36.0	32.0	36.0
27	34.946761792311534	36.0	36.0	36.0	32.0	36.0
28	34.9397396026472	36.0	36.0	36.0	32.0	36.0
29	34.919630053455094	36.0	36.0	36.0	32.0	36.0
30	34.91047542347368	36.0	36.0	36.0	32.0	36.0
31	34.906651894284956	36.0	36.0	36.0	32.0	36.0
32	34.880371766550866	36.0	36.0	36.0	32.0	36.0
33	34.85705369851753	36.0	36.0	36.0	32.0	36.0
34	34.854509481851885	36.0	36.0	36.0	32.0	36.0
35	34.824138890721535	36.0	36.0	36.0	32.0	36.0
36	34.79962947711987	36.0	36.0	36.0	32.0	36.0
37	34.792312294822764	36.0	36.0	36.0	32.0	36.0
38	34.76315200765616	36.0	36.0	36.0	32.0	36.0
39	34.752492839414046	36.0	36.0	36.0	32.0	36.0
40	34.73168410459878	36.0	36.0	36.0	32.0	36.0
41	34.70975075776707	36.0	36.0	36.0	32.0	36.0
42	34.68519432734475	36.0	36.0	36.0	32.0	36.0
43	34.67571740464117	36.0	36.0	36.0	32.0	36.0
44	34.656599758697546	36.0	36.0	36.0	32.0	36.0
45	34.60371417716393	36.0	36.0	36.0	32.0	36.0
46	34.62537528143738	36.0	36.0	36.0	32.0	36.0
47	34.600788517582394	36.0	36.0	36.0	32.0	36.0
48	34.56724201605093	36.0	36.0	36.0	32.0	36.0
49	34.56533100979239	36.0	36.0	36.0	32.0	36.0
50	34.05938679449175	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	28.0
22	76.0
23	256.0
24	671.0
25	1572.0
26	3390.0
27	6358.0
28	10880.0
29	17563.0
30	26627.0
31	39813.0
32	60212.0
33	102397.0
34	232011.0
35	816821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88293604144072	17.41405070875458	11.333854123214842	26.369159126589864
2	16.620798179922268	19.50509052990805	36.48429234998578	27.3898189401839
3	18.9555529097901	22.98620806282699	28.33417635509745	29.724062672285463
4	12.44605380400781	15.09606233529869	35.53127192067795	36.92661194001555
5	14.564446031894088	36.535709654449114	33.506158066001	15.393686247655793
6	35.78084618464637	34.829661962944535	15.919955834354562	13.469536018054528
7	31.050690237783957	30.025191934940803	20.111005281054634	18.813112546220605
8	28.539892634815047	33.35934425185243	18.688503704849634	19.412259408482896
9	26.858737962366824	14.07236192031862	18.120434344422478	40.94846577289207
10	15.425612185546575	27.060682790402808	31.89969947151577	25.614005552534852
11	38.45672594577747	20.72600037765124	21.30999479023294	19.50727888633835
12	24.189395886938197	23.46351684301766	28.812135193076088	23.534952076968054
13	29.7094739651939	19.012616432985485	25.040097006317698	26.23781259550292
14	23.064556369755447	19.28455565691978	24.953798390356397	32.69708958296839
15	25.51595273141186	27.116041305035278	21.864944941028014	25.503061022524847
16	26.247291793213957	25.785768615058878	23.2854595932135	24.681479998513662
17	24.427437499857813	26.003335160922653	24.92786330541899	24.641364033800546
18	25.293987837810167	24.866513937833147	25.77553108153096	24.063967142825724
19	25.572600416933035	25.460215048870953	24.81873878136951	24.148445752826508
20	25.49107931661809	24.464671788466774	25.033196150384057	25.01105274453107
21	26.3644546769224	24.528826998575088	24.25430943286339	24.85240889163912
22	25.399396516356926	24.617931457058855	24.81729794331743	25.16537408326679
23	24.455268424337422	24.281685355852876	25.306500378788744	25.95654584102096
24	24.648264889734183	25.244392675386013	24.84778304315613	25.259559391723673
25	25.011811080347957	24.61512561453639	24.859385581154445	25.513677723961216
26	24.79629204118977	25.08438381802367	25.232031801570816	24.887292339215744
27	25.551670348387056	24.8713672870612	24.5709146364121	25.00604772813964
28	24.809866252311977	24.60769392353093	25.271616931212115	25.310822892944973
29	24.669574126188596	25.057766230851076	25.24181433360861	25.030845309351722
30	24.841413022294315	25.026598628777176	25.476746769679004	24.655241579249505
31	25.12844312898458	24.76603444209613	24.632719005488077	25.472803423431213
32	24.821165455983536	24.829203815642494	24.70187923198782	25.64775149638615
33	24.598518060146645	24.495611889795608	25.145809019191205	25.760061030866545
34	25.169165762351202	24.793865366575744	25.332359630144456	24.704609240928598
35	25.785616947895505	24.551728740244958	25.10501055224289	24.557643759616646
36	24.749351054124702	25.103493880609122	24.89373819365925	25.25341687160692
37	25.710390034860698	24.74002352357704	24.689973359662755	24.85961308189951
38	24.842474692437953	24.662294102346518	25.11866059694679	25.376570608268743
39	25.257739385763156	24.680494161951714	24.746241877275484	25.31552457500965
40	25.516786900810434	24.840958020804184	24.99262518418081	24.64962989420457
41	24.658881591170545	25.021669445967436	25.450963351904974	24.86848561095704
42	25.851971331872782	25.191688336112634	24.705898411817298	24.25044192019729
43	25.13974233265614	24.235881872513133	25.05200287864276	25.57237291618797
44	24.88478983102003	24.77361780026496	25.047149529414707	25.294442839300295
45	25.03281698247562	24.97874763873185	24.985193493175355	25.00324188561718
46	24.956774825658464	24.704476308054442	25.11739047347491	25.22135839281218
47	25.147477357988347	24.57372047893457	25.426241604274587	24.852560558802498
48	25.569794574410565	25.33584797490212	24.40863077159911	24.68572667908821
49	24.678825823154572	25.03372698545588	25.027736132502497	25.259711058887053
50	24.694087099484634	25.41473417276116	24.731776418164884	25.159402309589314
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	12.0
2	16.0
3	51.0
4	86.0
5	103.5
6	121.0
7	105.0
8	89.0
9	121.5
10	154.0
11	257.0
12	360.0
13	629.5
14	899.0
15	1173.5
16	1448.0
17	1666.0
18	1884.0
19	2073.0
20	2262.0
21	2928.5
22	3595.0
23	4161.5
24	4728.0
25	6114.5
26	7501.0
27	9520.0
28	11539.0
29	13483.0
30	15427.0
31	17232.0
32	19037.0
33	22159.0
34	25281.0
35	29252.5
36	33224.0
37	40089.5
38	46955.0
39	51456.5
40	55958.0
41	61593.0
42	67228.0
43	69831.0
44	72434.0
45	80702.0
46	88970.0
47	96396.5
48	103823.0
49	105566.0
50	107309.0
51	103791.0
52	100273.0
53	98898.5
54	97524.0
55	97320.0
56	97116.0
57	93382.0
58	89648.0
59	82671.0
60	75694.0
61	65891.0
62	56088.0
63	48397.0
64	40706.0
65	34592.5
66	28479.0
67	24465.5
68	20452.0
69	18607.0
70	16762.0
71	12890.0
72	9018.0
73	8088.5
74	7159.0
75	5076.5
76	2994.0
77	2633.0
78	2272.0
79	2002.0
80	1732.0
81	1337.5
82	943.0
83	830.5
84	718.0
85	590.5
86	463.0
87	341.0
88	219.0
89	136.0
90	53.0
91	34.0
92	15.0
93	11.0
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012664208141948333
2	0.003943346247792295
3	6.066686535065069E-4
4	1.5166716337662673E-4
5	0.0
6	3.7916790844156684E-4
7	7.583358168831337E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.583358168831337E-5
47	0.0
48	0.0
49	0.0
50	7.583358168831337E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1318677.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.450298468975085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.51499338793228	50.09120673384969
2	11.792412086623786	14.492944847843617
3	3.0701481682617238	5.659845638509802
4	1.2217032962852254	3.002961287890312
5	0.6485844285374438	1.9927853357977778
6	0.37667136070222024	1.3887940523919844
7	0.2575079831301642	1.1076759695044673
8	0.18374322846833827	0.9032860984825972
9	0.1301485682221613	0.719790152630542
>10	0.6792912040837954	7.617283514393122
>50	0.059854562939033705	2.5680759142738907
>100	0.05812534978019474	7.333791713955436
>500	0.005700968209935604	2.272398499520498
>1k	0.001115406823683053	0.8491602409562824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1620	0.12285040233506765	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1400	0.1061670143636387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5166716337662673E-4
2	0.0	0.0	0.0	0.0	1.5166716337662673E-4
3	0.0	0.0	0.0	0.0	1.5166716337662673E-4
4	0.0	0.0	0.0	0.0	1.5166716337662673E-4
5	0.0	0.0	0.0	0.0	1.5166716337662673E-4
6	0.0	0.0	0.0	0.0	1.5166716337662673E-4
7	0.0	0.0	0.0	0.0	1.5166716337662673E-4
8	0.0	0.0	0.0	0.0	1.5166716337662673E-4
9	0.0	0.0	0.0	0.0	1.5166716337662673E-4
10	0.0	0.0	0.0	7.583358168831337E-5	1.5166716337662673E-4
11	0.0	0.0	0.0	7.583358168831337E-5	1.5166716337662673E-4
12	0.0	0.0	0.0	7.583358168831337E-5	3.791679084415668E-4
13	0.0	0.0	0.0	7.583358168831337E-5	4.5500149012988017E-4
14	0.0	0.0	0.0	7.583358168831337E-5	4.5500149012988017E-4
15	0.0	0.0	0.0	1.5166716337662673E-4	6.066686535065069E-4
16	0.0	0.0	0.0	1.5166716337662673E-4	6.066686535065069E-4
17	0.0	0.0	0.0	1.5166716337662673E-4	6.066686535065069E-4
18	0.0	0.0	0.0	1.5166716337662673E-4	6.066686535065069E-4
19	0.0	0.0	0.0	2.2750074506494008E-4	6.066686535065069E-4
20	0.0	0.0	0.0	2.2750074506494008E-4	7.583358168831336E-4
21	0.0	0.0	0.0	3.791679084415668E-4	7.583358168831336E-4
22	0.0	0.0	0.0	4.5500149012988017E-4	8.34169398571447E-4
23	0.0	0.0	0.0	6.066686535065069E-4	8.34169398571447E-4
24	0.0	0.0	0.0	8.34169398571447E-4	8.34169398571447E-4
25	0.0	0.0	0.0	0.0011375037253247005	9.100029802597603E-4
26	0.0	0.0	0.0	0.001440838052077954	9.100029802597603E-4
27	0.0	0.0	0.0	0.001668338797142894	9.100029802597603E-4
28	0.0	0.0	0.0	0.0030333432675325343	9.100029802597603E-4
29	0.0	0.0	0.0	0.004398347737922175	9.100029802597603E-4
30	0.0	0.0	0.0	0.008417527567402784	9.100029802597603E-4
31	0.0	0.0	0.0	0.01918589616714328	9.100029802597603E-4
32	0.0	0.0	0.0	0.03116760207389679	9.858365619480737E-4
33	0.0	0.0	0.0	0.04534848184961139	9.858365619480737E-4
34	0.0	0.0	0.0	0.05930186088026105	0.0011375037253247005
35	0.0	0.0	0.0	0.07848775704740434	0.0011375037253247005
36	0.0	0.0	0.0	0.10631868152701533	0.0011375037253247005
37	0.0	0.0	0.0	0.15181883054000336	0.0011375037253247005
38	0.0	0.0	0.0	0.21688404362857622	0.0011375037253247005
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	185	0.0	20.216007	15
TAACGCC	285	0.0	19.298046	4
TAGCGGC	350	0.0	18.856947	30
ATAACGC	315	0.0	18.158543	3
CTAGACA	170	1.2732926E-10	18.11746	4
TACCGTC	280	0.0	18.071241	7
AACGCCG	305	0.0	18.032598	5
ATACCGT	305	0.0	18.032598	6
TTAACGG	160	1.1023076E-9	17.874815	35
AATAACG	315	0.0	17.462124	2
CGCAAGA	535	0.0	17.272814	2
TAGGACG	90	2.2157384E-4	17.110933	4
AAGACGG	550	0.0	16.799826	5
CGCAATA	395	0.0	16.708689	36
CTAGCGG	400	0.0	16.49983	29
CGCCGGT	390	0.0	16.358805	7
TAACGGC	175	3.6270649E-9	16.342688	36
TACGAAT	405	0.0	16.296127	41
CGAATGC	405	0.0	16.296127	43
ACCGTCG	285	0.0	16.210358	8
>>END_MODULE