FastQCFastQC Report
Thu 2 Feb 2017
SRR4062532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365384
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG138043.7779432049569768No Hit
TCCATGTACTCTGCGTTGATACCAC112743.0855209861406085No Hit
GAGTACATGGAAGCAGTGGTATCAA58261.5944868959779301No Hit
GTATCAACGCAGAGTACTTTTTTTT55741.5255183587677623No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51721.4154971208372562No Hit
CATGTACTCTGCGTTGATACCACTG50431.3801917982177654No Hit
TATCAACGCAGAGTACTTTTTTTTT39251.0742123355155124No Hit
CCCATGTACTCTGCGTTGATACCAC36190.9904648260460228No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35170.962548989556193No Hit
GCTTCCATGTACTCTGCGTTGATAC34280.9381910537954591No Hit
GTACATGGGAAGCAGTGGTATCAAC33260.9102752173056292No Hit
GCGTTGATACCACTGCTTCCATGTA31210.8541698596545004No Hit
CATGGAAGCAGTGGTATCAACGCAG28530.7808223677008298No Hit
GTATCAACGCAGAGTACATGGAAGC27130.742506513695181No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25240.6907801107875551No Hit
GTACATGGGGTGGTATCAACGCAAA24210.6625905896262562No Hit
ACTCTGCGTTGATACCACTGCTTCC21370.5848641429290828No Hit
CAGTGGTATCAACGCAGAGTACATG20310.5558535677533772No Hit
ACGCAGAGTACATGGAAGCAGTGGT19960.5462746042519651No Hit
GGTATCAACGCAGAGTACTTTTTTT19750.5405272261511177No Hit
GTATCAACGCAGAGTACATGGGAAG18960.518906137105073No Hit
TATCAACGCAGAGTACATGGAAGCA16000.43789547435027254No Hit
GTACATGGGTGGTATCAACGCAAAA14730.4031375210737197No Hit
GTACATGGGAGTGGTATCAACGCAA13630.3730322072121385No Hit
ATACCACTGCTTCCATGTACTCTGC12060.33006371379151794No Hit
GCGTTGATACCACTGCTTCCCATGT11970.32760055174829766No Hit
TATCAACGCAGAGTACATGGGAAGC11960.32732686707682873No Hit
GAGTACATGGGAAGCAGTGGTATCA11820.32349528167626385No Hit
GTATCAACGCAGAGTACATGGGGTG11730.32103211963304357No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11190.3062531473737219No Hit
GGTATCAACGCAGAGTACATGGAAG10530.28818995905677314No Hit
AAAAAGTACTCTGCGTTGATACCAC10270.28107415759858123No Hit
AAGCAGTGGTATCAACGCAGAGTAC10140.2775162568694852No Hit
GGTATCAACGCAGAGTACATGGGAA10000.2736846714689204No Hit
GTACTCTGCGTTGATACCACTGCTT9830.2690320320539487No Hit
TATCAACGCAGAGTACATGGGGTGG8980.2457688349790905No Hit
GTGGTATCAACGCAGAGTACATGGA8970.24549515030762156No Hit
CCACTGCTTCCATGTACTCTGCGTT8780.2402951415497121No Hit
GCTTCCCATGTACTCTGCGTTGATA8540.233726709434458No Hit
GAAGCAGTGGTATCAACGCAGAGTA8500.23263197074858233No Hit
ACGCAGAGTACATGGGAAGCAGTGG8330.22797933133361067No Hit
ATCAACGCAGAGTACTTTTTTTTTT7730.21155825104547543No Hit
ACATGGAAGCAGTGGTATCAACGCA7350.20115823352965648No Hit
GTATCAACGCAGAGTACATGGGAGT6890.18856873864208612No Hit
CTGCTTCCATGTACTCTGCGTTGAT6850.18747399995621045No Hit
TGGAAGCAGTGGTATCAACGCAGAG6740.18446346857005233No Hit
GTTGATACCACTGCTTCCATGTACT6030.16503185689575897No Hit
CCCCATGTACTCTGCGTTGATACCA6010.16448448755282113No Hit
GCAGAGTACATGGAAGCAGTGGTAT5950.16284237952400762No Hit
GATACCACTGCTTCCATGTACTCTG5830.15955816346638058No Hit
GGTATCAACGCAGAGTACATGGGGT5470.14970551529349946No Hit
CCATGTACTCTGCGTTGATACCACT5270.14423182186412103No Hit
ACGCAGAGTACATGGGGTGGTATCA5250.1436844525211832No Hit
CCATAGGGTCTTCTCGTCTTATTAT5220.14286339850677643No Hit
GTATTAGAGGCACTGCCTGCCCAGT5120.14012655179208722No Hit
GTATCAACGCAGAGTACATGGGCAG5110.1398528671206183No Hit
CTTCCATGTACTCTGCGTTGATACC4970.13602128172005343No Hit
GTGGTATCAACGCAGAGTACATGGG4900.13410548901977098No Hit
GTATCAACGCAGAGTACATGGGTGG4790.13109495763361287No Hit
ATCAACGCAGAGTACATGGAAGCAG4720.12917916493333043No Hit
GCAGTGGTATCAACGCAGAGTACAT4620.12644231821864121No Hit
TATCAACGCAGAGTACATGGGAGTG4520.123705471503952No Hit
GAGTACATGGGGTGGTATCAACGCA4470.12233704814660741No Hit
AAAGTACTCTGCGTTGATACCACTG4310.11795809340310467No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT4130.11303176931666412No Hit
CTCTAATACTTGTAATGCTAGAGGT4120.1127580846451952No Hit
CTGCTTCCCATGTACTCTGCGTTGA3800.10400017515818974No Hit
TATCAACGCAGAGTACATGGGTGGT3750.10263175180084513No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3690.10098964377203161No Hit
CAACGCAGAGTACTTTTTTTTTTTT3670.10044227442909377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCAA307.696043E-419.00411417
ACGGCTA250.006020667519.0015098
GACAGAG350.002164773916.2870067
AGTGTGT350.002164773916.2870068
TAAGGTT350.002166757416.2847754
GCTCTAA350.002172716716.2780821
AGGTTGT508.6789696E-515.2012066
AAGTGTG456.740739E-414.7789517
ATATATC456.7547674E-414.77493
ATACCAA602.5524321E-514.25308519
CCCAAGG400.00526130114.25308419
CACAAAT400.00526130114.25308419
TTTTAAG400.00526130114.25308418
AACCGAC400.005266089514.2511317
ACCGACC400.005266089514.2511318
GTTGTTT400.005266089514.2511318
GGAGTGT400.005266089514.2511316
CTAAGTT400.005270881614.2491784
CATGAAC400.005275677414.2472263
TTCTAAG400.0052852814.2433222
Produced by FastQC (version 0.11.5)