##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062532_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	365384
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18951021391194	32.0	32.0	32.0	21.0	32.0
2	30.291553543669128	32.0	32.0	32.0	21.0	32.0
3	30.387313073369388	32.0	32.0	32.0	27.0	32.0
4	30.202324130230114	32.0	32.0	32.0	21.0	32.0
5	30.324891073500755	32.0	32.0	32.0	27.0	32.0
6	33.68047040921332	36.0	36.0	36.0	27.0	36.0
7	33.28013268232873	36.0	36.0	36.0	21.0	36.0
8	33.202072340332364	36.0	36.0	36.0	14.0	36.0
9	33.17299881768222	36.0	36.0	36.0	14.0	36.0
10	33.200720338055305	36.0	36.0	36.0	21.0	36.0
11	33.60854060385786	36.0	36.0	36.0	27.0	36.0
12	33.40888216232785	36.0	36.0	36.0	21.0	36.0
13	33.45443150220042	36.0	36.0	36.0	21.0	36.0
14	33.400170779235	36.0	36.0	36.0	21.0	36.0
15	33.52039771856458	36.0	36.0	36.0	27.0	36.0
16	33.38878002320846	36.0	36.0	36.0	21.0	36.0
17	33.29701355286493	36.0	36.0	36.0	21.0	36.0
18	33.39514319182011	36.0	36.0	36.0	21.0	36.0
19	33.32154117312198	36.0	36.0	36.0	21.0	36.0
20	33.149724673220504	36.0	36.0	36.0	14.0	36.0
21	32.97907954371291	36.0	36.0	36.0	14.0	36.0
22	32.97265616447354	36.0	36.0	36.0	14.0	36.0
23	32.94312832526876	36.0	36.0	36.0	14.0	36.0
24	32.99272272458564	36.0	36.0	36.0	14.0	36.0
25	32.39555919252074	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	20.0
5	29.0
6	54.0
7	17.0
8	61.0
9	79.0
10	38.0
11	14.0
12	33.0
13	16.0
14	312.0
15	327.0
16	420.0
17	592.0
18	837.0
19	1263.0
20	1786.0
21	2519.0
22	3435.0
23	4240.0
24	4909.0
25	5820.0
26	6981.0
27	8232.0
28	9948.0
29	12051.0
30	14919.0
31	19685.0
32	27041.0
33	36375.0
34	72285.0
35	131043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.618326335554016	17.563513742835717	13.686999458059853	20.13116046355042
2	11.710562688335965	24.02927435401277	38.582974565578546	25.677188392072715
3	19.94526096833347	32.42001258997728	27.269343405315162	20.365383036374087
4	11.231215613281142	18.518599622259327	34.019927189116686	36.23025757534284
5	10.630550718924187	41.54100550828433	32.6190632631383	15.209380509653187
6	29.075217943663205	33.39493801200333	21.467998422920225	16.06184562141324
7	25.33570694967477	34.92064361520028	22.57336528586101	17.17028414926395
8	30.71516227291653	28.761806149058796	22.59192908640677	17.93110249161791
9	28.445016711413075	17.41356637992439	19.860007670812557	34.28140923784998
10	16.70868247557146	28.72648256435269	31.471028272985848	23.093806687090005
11	31.754660937996647	25.551037408069092	20.177395135745364	22.516906518188893
12	22.750019183758482	28.2124573846508	29.008857415344814	20.028666016245904
13	31.388877471330513	22.492498664145668	26.310164823872746	19.808459040651076
14	26.32163056822168	20.486506927393897	26.84392537706068	26.347937127323746
15	26.388945971049754	23.821070715207508	32.24064377717682	17.54933953656592
16	23.769350905553882	27.393210400854866	28.574951365865687	20.262487327725566
17	20.766324050452546	26.230828882793467	31.34678040319288	21.65606666356111
18	23.66846716488672	23.142842265942082	34.41445441067254	18.774236158498653
19	20.31284683494699	33.30355405623899	29.484224747675437	16.89937436113859
20	21.44963198824993	22.36020759918232	37.28509812733261	18.90506228523514
21	22.851959639805543	27.792575784979473	26.128622086299785	23.226842488915196
22	20.31762216447938	29.273701060202395	27.759047900974686	22.64962887434354
23	19.14282503775286	28.672990328141573	27.950273108725504	24.233911525380066
24	18.335512054218633	33.11765366833565	27.980531279032483	20.56630299841323
25	23.874891065448427	26.83407417963376	26.237743150140584	23.053291604777225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	525.0
1	525.0
2	1008.0
3	1491.0
4	1491.0
5	1491.0
6	4041.0
7	6591.0
8	6591.0
9	6591.0
10	5999.0
11	5407.0
12	5407.0
13	5407.0
14	3782.0
15	2157.0
16	2157.0
17	2157.0
18	3360.0
19	4563.0
20	4563.0
21	4563.0
22	6808.5
23	9054.0
24	9054.0
25	9054.0
26	12793.0
27	16532.0
28	16532.0
29	16532.0
30	18235.0
31	19938.0
32	19938.0
33	19938.0
34	20418.5
35	20899.0
36	20899.0
37	20899.0
38	21426.5
39	21954.0
40	21954.0
41	21954.0
42	29526.0
43	37098.0
44	37098.0
45	37098.0
46	69869.0
47	102640.0
48	102640.0
49	102640.0
50	78212.0
51	53784.0
52	53784.0
53	53784.0
54	37866.5
55	21949.0
56	21949.0
57	21949.0
58	18125.0
59	14301.0
60	14301.0
61	14301.0
62	12368.0
63	10435.0
64	10435.0
65	10435.0
66	8762.0
67	7089.0
68	7089.0
69	7089.0
70	5721.5
71	4354.0
72	4354.0
73	4354.0
74	3421.0
75	2488.0
76	2488.0
77	2488.0
78	1863.0
79	1238.0
80	1238.0
81	1238.0
82	836.5
83	435.0
84	435.0
85	435.0
86	316.0
87	197.0
88	197.0
89	197.0
90	130.0
91	63.0
92	63.0
93	63.0
94	45.5
95	28.0
96	28.0
97	28.0
98	101.0
99	174.0
100	174.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00821054014406761
2	0.003557900729095965
3	0.0038315854005648853
4	0.0147789722593217
5	0.03174742189039476
6	0.0416000700632759
7	0.07088432991045038
8	0.08757909487005452
9	0.09962122041468702
10	0.1209686247892628
11	0.11932651676044928
12	0.13465285836270882
13	0.12288441748954525
14	0.12480021018982769
15	0.11303176931666412
16	0.11330545398813302
17	0.12233704814660741
18	0.13273706566242638
19	0.13054758829067503
20	0.12315810216101417
21	0.1286317955903926
22	0.14997919996496836
23	0.1398528671206183
24	0.1343791736912399
25	0.1330107503338953
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	365384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.726676592297416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65465157954021	19.95270728877017
2	12.976294096564436	6.9362643137083175
3	4.416568532077211	3.5412059641363602
4	2.0521222671650197	2.1938563264948656
5	1.1458706671445396	1.5312657368686093
6	0.7065690440837643	1.1330545398813303
7	0.4792381342481184	0.896590983732183
8	0.349188469612411	0.7466117837672147
9	0.28569965695560906	0.6872222100584591
>10	2.3583021862679843	12.820484750290106
>50	0.29184373559981563	5.368051146191404
>100	0.2263068967282781	11.609703763711602
>500	0.02355230146945881	4.454218028156679
>1k	0.027648353898929903	15.349604799334399
>5k	0.004096052429471097	5.915694173800714
>10k+	0.0020480262147355485	6.863464191097584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	13804	3.7779432049569768	No Hit
TCCATGTACTCTGCGTTGATACCAC	11274	3.0855209861406085	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5826	1.5944868959779301	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5574	1.5255183587677623	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5172	1.4154971208372562	No Hit
CATGTACTCTGCGTTGATACCACTG	5043	1.3801917982177654	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3925	1.0742123355155124	No Hit
CCCATGTACTCTGCGTTGATACCAC	3619	0.9904648260460228	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3517	0.962548989556193	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3428	0.9381910537954591	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3326	0.9102752173056292	No Hit
GCGTTGATACCACTGCTTCCATGTA	3121	0.8541698596545004	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2853	0.7808223677008298	No Hit
GTATCAACGCAGAGTACATGGAAGC	2713	0.742506513695181	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2524	0.6907801107875551	No Hit
GTACATGGGGTGGTATCAACGCAAA	2421	0.6625905896262562	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2137	0.5848641429290828	No Hit
CAGTGGTATCAACGCAGAGTACATG	2031	0.5558535677533772	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1996	0.5462746042519651	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1975	0.5405272261511177	No Hit
GTATCAACGCAGAGTACATGGGAAG	1896	0.518906137105073	No Hit
TATCAACGCAGAGTACATGGAAGCA	1600	0.43789547435027254	No Hit
GTACATGGGTGGTATCAACGCAAAA	1473	0.4031375210737197	No Hit
GTACATGGGAGTGGTATCAACGCAA	1363	0.3730322072121385	No Hit
ATACCACTGCTTCCATGTACTCTGC	1206	0.33006371379151794	No Hit
GCGTTGATACCACTGCTTCCCATGT	1197	0.32760055174829766	No Hit
TATCAACGCAGAGTACATGGGAAGC	1196	0.32732686707682873	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1182	0.32349528167626385	No Hit
GTATCAACGCAGAGTACATGGGGTG	1173	0.32103211963304357	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1119	0.3062531473737219	No Hit
GGTATCAACGCAGAGTACATGGAAG	1053	0.28818995905677314	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1027	0.28107415759858123	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1014	0.2775162568694852	No Hit
GGTATCAACGCAGAGTACATGGGAA	1000	0.2736846714689204	No Hit
GTACTCTGCGTTGATACCACTGCTT	983	0.2690320320539487	No Hit
TATCAACGCAGAGTACATGGGGTGG	898	0.2457688349790905	No Hit
GTGGTATCAACGCAGAGTACATGGA	897	0.24549515030762156	No Hit
CCACTGCTTCCATGTACTCTGCGTT	878	0.2402951415497121	No Hit
GCTTCCCATGTACTCTGCGTTGATA	854	0.233726709434458	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	850	0.23263197074858233	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	833	0.22797933133361067	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	773	0.21155825104547543	No Hit
ACATGGAAGCAGTGGTATCAACGCA	735	0.20115823352965648	No Hit
GTATCAACGCAGAGTACATGGGAGT	689	0.18856873864208612	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	685	0.18747399995621045	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	674	0.18446346857005233	No Hit
GTTGATACCACTGCTTCCATGTACT	603	0.16503185689575897	No Hit
CCCCATGTACTCTGCGTTGATACCA	601	0.16448448755282113	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	595	0.16284237952400762	No Hit
GATACCACTGCTTCCATGTACTCTG	583	0.15955816346638058	No Hit
GGTATCAACGCAGAGTACATGGGGT	547	0.14970551529349946	No Hit
CCATGTACTCTGCGTTGATACCACT	527	0.14423182186412103	No Hit
ACGCAGAGTACATGGGGTGGTATCA	525	0.1436844525211832	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	522	0.14286339850677643	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	512	0.14012655179208722	No Hit
GTATCAACGCAGAGTACATGGGCAG	511	0.1398528671206183	No Hit
CTTCCATGTACTCTGCGTTGATACC	497	0.13602128172005343	No Hit
GTGGTATCAACGCAGAGTACATGGG	490	0.13410548901977098	No Hit
GTATCAACGCAGAGTACATGGGTGG	479	0.13109495763361287	No Hit
ATCAACGCAGAGTACATGGAAGCAG	472	0.12917916493333043	No Hit
GCAGTGGTATCAACGCAGAGTACAT	462	0.12644231821864121	No Hit
TATCAACGCAGAGTACATGGGAGTG	452	0.123705471503952	No Hit
GAGTACATGGGGTGGTATCAACGCA	447	0.12233704814660741	No Hit
AAAGTACTCTGCGTTGATACCACTG	431	0.11795809340310467	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	413	0.11303176931666412	No Hit
CTCTAATACTTGTAATGCTAGAGGT	412	0.1127580846451952	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	380	0.10400017515818974	No Hit
TATCAACGCAGAGTACATGGGTGGT	375	0.10263175180084513	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	369	0.10098964377203161	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	367	0.10044227442909377	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.7368467146892035E-4	0.0
9	0.0	0.0	0.0	2.7368467146892035E-4	0.0
10	0.0	0.0	0.0	2.7368467146892035E-4	0.0
11	0.0	0.0	0.0	5.473693429378407E-4	0.0
12	0.0	0.0	0.0	5.473693429378407E-4	0.0
13	0.0	0.0	0.0	8.210540144067611E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCCAA	30	7.696043E-4	19.004114	17
ACGGCTA	25	0.0060206675	19.001509	8
GACAGAG	35	0.0021647739	16.287006	7
AGTGTGT	35	0.0021647739	16.287006	8
TAAGGTT	35	0.0021667574	16.284775	4
GCTCTAA	35	0.0021727167	16.278082	1
AGGTTGT	50	8.6789696E-5	15.201206	6
AAGTGTG	45	6.740739E-4	14.778951	7
ATATATC	45	6.7547674E-4	14.7749	3
ATACCAA	60	2.5524321E-5	14.253085	19
CCCAAGG	40	0.005261301	14.253084	19
CACAAAT	40	0.005261301	14.253084	19
TTTTAAG	40	0.005261301	14.253084	18
AACCGAC	40	0.0052660895	14.251131	7
ACCGACC	40	0.0052660895	14.251131	8
GTTGTTT	40	0.0052660895	14.251131	8
GGAGTGT	40	0.0052660895	14.251131	6
CTAAGTT	40	0.0052708816	14.249178	4
CATGAAC	40	0.0052756774	14.247226	3
TTCTAAG	40	0.00528528	14.243322	2
>>END_MODULE