##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062532_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	365384
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.5316735270291	32.0	32.0	32.0	32.0	32.0
2	31.318610010290545	32.0	32.0	32.0	32.0	32.0
3	31.468706894664244	32.0	32.0	32.0	32.0	32.0
4	31.625528211415936	32.0	32.0	32.0	32.0	32.0
5	31.542103649858777	32.0	32.0	32.0	32.0	32.0
6	35.08339719309001	36.0	36.0	36.0	36.0	36.0
7	35.098277428677775	36.0	36.0	36.0	36.0	36.0
8	35.07836960567513	36.0	36.0	36.0	36.0	36.0
9	35.06932432728308	36.0	36.0	36.0	36.0	36.0
10	35.053579248133474	36.0	36.0	36.0	36.0	36.0
11	35.12477831541611	36.0	36.0	36.0	36.0	36.0
12	35.02755183587678	36.0	36.0	36.0	36.0	36.0
13	35.0248916208701	36.0	36.0	36.0	36.0	36.0
14	34.95846835110459	36.0	36.0	36.0	36.0	36.0
15	34.99834694458433	36.0	36.0	36.0	36.0	36.0
16	34.95964519519191	36.0	36.0	36.0	36.0	36.0
17	34.957981192389376	36.0	36.0	36.0	36.0	36.0
18	34.91836259934754	36.0	36.0	36.0	36.0	36.0
19	34.821324962231515	36.0	36.0	36.0	32.0	36.0
20	34.712409410373745	36.0	36.0	36.0	32.0	36.0
21	34.59033509951175	36.0	36.0	36.0	32.0	36.0
22	34.57456538874171	36.0	36.0	36.0	32.0	36.0
23	34.49016377290741	36.0	36.0	36.0	32.0	36.0
24	34.478012173494186	36.0	36.0	36.0	32.0	36.0
25	34.46195509381911	36.0	36.0	36.0	32.0	36.0
26	34.359391763186125	36.0	36.0	36.0	32.0	36.0
27	34.293617126092	36.0	36.0	36.0	32.0	36.0
28	34.18887526547413	36.0	36.0	36.0	32.0	36.0
29	34.11951262233705	36.0	36.0	36.0	32.0	36.0
30	34.04606934074836	36.0	36.0	36.0	32.0	36.0
31	33.953090447310224	36.0	36.0	36.0	32.0	36.0
32	33.82748286733957	36.0	36.0	36.0	32.0	36.0
33	33.65956911085324	36.0	36.0	36.0	27.0	36.0
34	33.50520274560463	36.0	36.0	36.0	21.0	36.0
35	33.25354421649552	36.0	36.0	36.0	14.0	36.0
36	33.17915672279027	36.0	36.0	36.0	14.0	36.0
37	32.99378188426422	36.0	36.0	36.0	14.0	36.0
38	32.81342642261292	36.0	36.0	36.0	14.0	36.0
39	32.4071771068246	36.0	36.0	36.0	14.0	36.0
40	32.08868478094279	36.0	36.0	36.0	14.0	36.0
41	32.12961706040768	36.0	36.0	36.0	14.0	36.0
42	31.621026098570272	36.0	32.0	36.0	14.0	36.0
43	31.683281150789306	36.0	32.0	36.0	14.0	36.0
44	31.6180128303374	36.0	32.0	36.0	14.0	36.0
45	31.015205920346812	36.0	32.0	36.0	14.0	36.0
46	31.249833052350404	36.0	32.0	36.0	14.0	36.0
47	30.855573862019135	36.0	32.0	36.0	14.0	36.0
48	30.858053445142644	36.0	32.0	36.0	14.0	36.0
49	30.87331957611718	36.0	32.0	36.0	14.0	36.0
50	30.11412103430911	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	6.0
21	19.0
22	70.0
23	275.0
24	826.0
25	2045.0
26	4224.0
27	8124.0
28	13301.0
29	17462.0
30	21537.0
31	25824.0
32	34735.0
33	35372.0
34	50921.0
35	150640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.57927583677313	18.37260354964799	14.491749969890403	20.55637064368848
2	11.667816205209052	24.25717914189685	37.710611882944136	26.36439276994997
3	19.75932038250379	31.690121571396514	28.01150576656759	20.539052279532104
4	11.31579739615691	18.57010315203499	33.97503441594163	36.13906503586647
5	10.553280931841568	40.58989994088411	33.30441398638145	15.55240514089287
6	29.488154927418826	33.63174085345828	20.417423860924398	16.462680358198497
7	24.45536750377685	34.357552602193856	23.253070742013882	17.934009152015413
8	30.270345718477	28.55489019770981	23.016607185864736	18.15815689794846
9	27.872046942394853	16.447080331924766	20.443150220042476	35.237722505637905
10	16.774407199001597	27.982341864996823	32.13550675453769	23.107744181463886
11	31.82377991373459	24.706610032185317	20.55399251198739	22.915617542092704
12	22.417785124690738	28.10960523722987	29.631839379940004	19.840770258139383
13	31.818306220305214	21.760941913165325	26.97545595866267	19.445295907866793
14	26.205033608477656	19.83365445668119	27.764488866507563	26.19682306833359
15	26.64457119085674	23.248965471941847	32.65003393689926	17.45642940030215
16	23.695345171107657	26.245265255183586	29.374575788759223	20.684813784949533
17	20.509655595209423	25.39054802618615	32.54165480699757	21.558141571606857
18	24.00898780461104	21.28555163882381	36.02456593611105	18.680894620454097
19	20.560013574759704	31.63411643640663	31.171041972281216	16.63482801655245
20	21.165404068048957	20.88788781117947	39.02661309745364	18.920095023317934
21	22.418332494033674	26.64457119085674	27.997120837256144	22.939975477853437
22	20.030160050795875	27.48286733956605	29.765397499616842	22.721575110021238
23	19.117421671447026	26.456549821557594	29.89485034922164	24.531178157773738
24	18.372451995708623	31.247126310949575	29.89238718717842	20.48803450616338
25	24.08178792722177	24.161430166619226	28.179121143782982	23.57766076237602
26	18.059356731548178	24.304019880454536	32.46529678365774	25.171326604339544
27	23.540713331727716	28.405184682416305	31.168305125566526	16.88579686028945
28	21.00009852648173	22.567764324655705	32.34159131215379	24.090545836708777
29	18.26133601909224	28.421605762704445	35.37894379611587	17.938114422087448
30	24.358209445405382	24.307030411840692	33.1481400389727	18.18662010378123
31	17.296597552164297	26.829034659426792	31.841295732708602	24.033072055700305
32	17.692619271779826	28.440490005035794	32.1574015282552	21.709489194929173
33	18.425546821973597	27.372025047621136	31.64889540866595	22.55353272173932
34	16.954491712828148	29.143585926039457	34.54174238609244	19.360179975039955
35	22.53300637137915	27.475204168764915	30.873273049723032	19.1185164101329
36	20.768287609747553	28.789711645830142	30.923083659930374	19.51891708449193
37	22.504543165546384	28.774658988899347	31.031736474503536	17.68906137105073
38	20.723950692969588	29.158364898298778	27.96728920806604	22.150395200665603
39	18.39571519278348	29.16767017712872	31.77834825827075	20.658266371817046
40	21.19687780526788	29.33790204278239	28.149015829921396	21.31620432202833
41	17.423587239725876	29.36308103255753	30.588367306723885	22.62496442099271
42	22.938607054496092	29.597902480677863	29.410702165393122	18.052788299432926
43	21.49108882709697	26.59968690473584	27.11175092505419	24.797473343113
44	20.62213999518315	27.16238258927594	33.36818251483371	18.8472949007072
45	22.71363825454864	27.508046329341184	29.14221750268211	20.636097913428063
46	20.102412804063672	27.75244674096293	31.751800845138266	20.39333960983513
47	22.5995117465461	26.647855406914367	31.302684299257766	19.449948547281764
48	19.44338011516651	35.56504937271473	24.996168414599435	19.99540209751932
49	18.217546471657215	27.948952335077614	34.003404637313075	19.830096555952096
50	17.251986950714866	34.83485866923565	24.52351498697261	23.389639393076873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	60.5
2	51.0
3	213.5
4	376.0
5	412.5
6	449.0
7	438.0
8	427.0
9	550.5
10	674.0
11	1036.5
12	1399.0
13	2432.5
14	3466.0
15	4910.5
16	6355.0
17	7509.5
18	8664.0
19	9172.5
20	9681.0
21	9829.0
22	9977.0
23	9497.5
24	9018.0
25	7614.0
26	6210.0
27	6218.5
28	6227.0
29	6633.5
30	7040.0
31	7378.0
32	7716.0
33	8841.0
34	9966.0
35	10173.5
36	10381.0
37	11669.0
38	12957.0
39	13066.5
40	13176.0
41	15427.5
42	17679.0
43	20136.0
44	22593.0
45	29851.5
46	37110.0
47	42680.5
48	48251.0
49	41672.0
50	35093.0
51	26960.0
52	18827.0
53	14930.5
54	11034.0
55	10492.5
56	9951.0
57	9539.0
58	9127.0
59	8250.0
60	7373.0
61	6721.0
62	6069.0
63	5292.5
64	4516.0
65	4245.5
66	3975.0
67	3432.5
68	2890.0
69	2574.5
70	2259.0
71	1898.0
72	1537.0
73	1340.0
74	1143.0
75	942.5
76	742.0
77	610.0
78	478.0
79	346.5
80	215.0
81	152.5
82	90.0
83	84.0
84	78.0
85	56.0
86	34.0
87	31.5
88	29.0
89	17.0
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01423160291638386
2	0.005473693429378408
3	5.473693429378407E-4
4	2.7368467146892035E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	365384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.61283588700939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9029387248485	30.85956326823172
2	11.305481129784628	8.956843372356799
3	3.872556495047762	4.602088347044978
4	1.795664741004831	2.845254907740544
5	0.9838000016092472	1.9485554004693342
6	0.6687293352478412	1.5894159246000967
7	0.494829078126284	1.3721108144755645
8	0.33084255221977543	1.0484489380417037
9	0.282227984287266	1.0061865741863638
>10	1.9559373963694024	15.277362111701473
>50	0.23227003081302522	6.347230217458745
>100	0.15322888599904053	12.431892192256711
>500	0.013173524135664118	4.1593446306012645
>1k	0.006933433755612693	4.432624339228329
>5k	0.0013866867511225386	3.1230789616063777
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6091	1.667013333917194	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5280	1.4450550653558996	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2409	0.6593063735686292	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2225	0.6089483940183479	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1958	0.5358745867361461	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1576	0.43132704223501855	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1438	0.3935585575723075	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1394	0.381516432027675	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1347	0.36865325246863573	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1286	0.3519584875090316	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1271	0.3478532174369978	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1235	0.33800056926411665	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1000	0.2736846714689204	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	991	0.27122150942570006	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	947	0.2591793838810676	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	937	0.2564425371663784	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	920	0.2517898977514067	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	891	0.24385304227880805	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	879	0.240568826221181	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	871	0.23837934884942966	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	809	0.2214108992183566	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	784	0.21456878243163355	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	762	0.20854771965931732	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	728	0.19924244082937403	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	720	0.19705296345762266	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	716	0.19595822477174699	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	676	0.18501083791299017	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	650	0.17789503645479823	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	650	0.17789503645479823	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	613	0.16776870361044818	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	600	0.16421080288135223	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	492	0.13465285836270882	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	488	0.13355811967683315	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	481	0.1316423269765507	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	471	0.1289054802618615	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	466	0.1275370569045169	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	458	0.12534757953276554	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	458	0.12534757953276554	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	435	0.11905283208898036	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	430	0.11768440873163576	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	418	0.11440019267400872	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	414	0.11330545398813302	No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA	407	0.11138966128785058	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	401	0.10974755325903708	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	391	0.10701070654434786	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	383	0.1048212291725965	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	382	0.10454754450112758	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	367	0.10044227442909377	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	366	0.10016858975762485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.7368467146892035E-4	0.0
12	0.0	0.0	0.0	2.7368467146892035E-4	0.0
13	0.0	0.0	0.0	5.473693429378407E-4	0.0
14	0.0	0.0	0.0	5.473693429378407E-4	0.0
15	2.7368467146892035E-4	0.0	0.0	5.473693429378407E-4	0.0
16	2.7368467146892035E-4	0.0	0.0	0.001368423357344602	0.0
17	2.7368467146892035E-4	0.0	0.0	0.001368423357344602	0.0
18	2.7368467146892035E-4	0.0	0.0	0.0024631620432202833	0.0
19	2.7368467146892035E-4	0.0	0.0	0.0035579007290959647	0.0
20	2.7368467146892035E-4	0.0	0.0	0.004926324086440567	0.0
21	2.7368467146892035E-4	0.0	0.0	0.0073894861296608496	0.0
22	2.7368467146892035E-4	0.0	0.0	0.014505287587852779	0.0
23	2.7368467146892035E-4	0.0	0.0	0.025452674446609595	0.0
24	2.7368467146892035E-4	0.0	0.0	0.04515797079237186	0.0
25	2.7368467146892035E-4	0.0	0.0	0.058021150351411116	0.0
26	2.7368467146892035E-4	0.0	0.0	0.07471591531101526	0.0
27	2.7368467146892035E-4	0.0	0.0	0.1020843824579073	0.0
28	2.7368467146892035E-4	0.0	0.0	0.13602128172005343	0.0
29	2.7368467146892035E-4	0.0	0.0	0.19267400871411994	0.0
30	2.7368467146892035E-4	0.0	0.0	0.29612681452937184	0.0
31	2.7368467146892035E-4	0.0	0.0	0.45349550062400107	0.0
32	2.7368467146892035E-4	0.0	0.0	0.6497274100672169	0.0
33	2.7368467146892035E-4	0.0	0.0	0.797517132660434	0.0
34	2.7368467146892035E-4	0.0	0.0	1.0233069866222932	0.0
35	2.7368467146892035E-4	0.0	0.0	1.2466336785409322	0.0
36	2.7368467146892035E-4	0.0	0.0	1.5805289777330152	0.0
37	2.7368467146892035E-4	0.0	0.0	2.0531824053598404	0.0
38	2.7368467146892035E-4	0.0	0.0	2.499835789197119	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGTA	25	0.0023507953	35.199562	42
TCGTATA	65	4.656613E-10	33.845734	44
ATACATG	100	0.0	33.01314	1
CGAGTAG	40	1.81677E-5	32.99959	39
TAGGCAG	40	1.81677E-5	32.99959	43
TAGTACA	35	3.213737E-4	31.42818	28
TATCACG	30	0.0057363454	29.336985	2
ATTAAGA	60	2.9326657E-7	29.336985	2
CCGTCCG	30	0.0057401843	29.332968	40
GTAGACC	30	0.0057401843	29.332968	3
TGCTATA	30	0.0057401843	29.332968	44
AGTAGGC	45	4.062211E-5	29.332968	41
GGTAGAA	45	4.062211E-5	29.332968	20
CCCCAAT	70	3.576679E-8	28.285362	44
GGGGCAT	40	7.02319E-4	27.499659	19
TCGTATT	40	7.02319E-4	27.499659	26
AGGTCCG	40	7.02319E-4	27.499659	36
GATTAAG	65	5.891616E-7	27.08771	1
TTTGATA	90	5.11136E-10	26.888554	44
AGCTATC	60	9.729632E-6	25.666346	38
>>END_MODULE