FastQCFastQC Report
Thu 2 Feb 2017
SRR4062531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419730
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30500.12604712095977652No Hit
GCGCAAGACGGACCAGAGCGAAAGC28730.11873225525161898No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27020.11166535109289052No Hit
GAATAGGACCGCGGTTCTATTTTGT26060.10769796630202544No Hit
GAATAACGCCGCCGCATCGCCAGTC25850.1068301008790237No Hit
GAACTACGACGGTATCTGATCGTCT25650.10600356238092679No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG25560.1056316200567832No Hit
GATTAAGAGGGACGGCCGGGGGCAT24680.10199485066515684No Hit
GTCCTATTCCATTATTCCTAGCTGC24510.10129229294177448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA350.002172911816.2831954
GCGTTAT2300.013.6215621
CGTTATT2250.013.0822632
AAGACGG4550.012.7345575
CAAGACG4700.012.7320744
AACCGCG2150.012.3722297
AATCGTA701.089587E-412.21644113
CGCATCG4400.012.09304313
CGCAAGA4700.011.9194962
CGTCGTA3500.011.67203910
ATCGCCA4300.011.49135216
CGAACGA2250.011.40295716
TAACGAA2250.011.40201213
GTATTAG5450.011.32291
TATACCG600.00588312111.0818495
GCAAGAC6000.011.07955553
ATTAGAC600.00589218811.07955553
TCGCCAG4550.011.06880417
CCGTCGT3450.011.0150699
AGAACCG3200.010.9829045
Produced by FastQC (version 0.11.5)