##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062531_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2419730
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195036636318928	32.0	32.0	32.0	32.0	32.0
2	30.797664202204377	32.0	32.0	32.0	32.0	32.0
3	30.775923760088936	32.0	32.0	32.0	32.0	32.0
4	30.866299959086344	32.0	32.0	32.0	32.0	32.0
5	30.623130266599993	32.0	32.0	32.0	32.0	32.0
6	34.28407177660318	36.0	36.0	36.0	32.0	36.0
7	34.1640187128316	36.0	36.0	36.0	32.0	36.0
8	34.177973988833465	36.0	36.0	36.0	32.0	36.0
9	34.34965843296566	36.0	36.0	36.0	32.0	36.0
10	33.97350324209726	36.0	36.0	36.0	32.0	36.0
11	34.32598430403392	36.0	36.0	36.0	32.0	36.0
12	34.066022655420234	36.0	36.0	36.0	32.0	36.0
13	34.20724791608981	36.0	36.0	36.0	32.0	36.0
14	34.10969529658268	36.0	36.0	36.0	32.0	36.0
15	34.02599546230365	36.0	36.0	36.0	32.0	36.0
16	34.03727812607192	36.0	36.0	36.0	32.0	36.0
17	33.96505188595422	36.0	36.0	36.0	32.0	36.0
18	33.973043273423066	36.0	36.0	36.0	32.0	36.0
19	33.95300095465196	36.0	36.0	36.0	32.0	36.0
20	33.96486798113839	36.0	36.0	36.0	32.0	36.0
21	33.95378781930216	36.0	36.0	36.0	32.0	36.0
22	33.89312237315733	36.0	36.0	36.0	32.0	36.0
23	33.85979592764482	36.0	36.0	36.0	32.0	36.0
24	33.82301413794101	36.0	36.0	36.0	32.0	36.0
25	33.36691407719043	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	13.0
4	94.0
5	236.0
6	399.0
7	116.0
8	352.0
9	463.0
10	274.0
11	77.0
12	155.0
13	136.0
14	298.0
15	378.0
16	604.0
17	865.0
18	1180.0
19	1708.0
20	2802.0
21	4327.0
22	6692.0
23	10231.0
24	14802.0
25	21276.0
26	29859.0
27	38992.0
28	53456.0
29	70518.0
30	91645.0
31	124387.0
32	178124.0
33	255560.0
34	554309.0
35	955400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08815871047737	17.437073775573467	10.892498450092994	26.582269063856167
2	17.277330901008543	19.792216422903806	36.24219044789024	26.688262228197416
3	19.343283952199005	23.42037886192638	28.04289112110811	29.193446064766505
4	12.845508763242616	15.04583636959585	35.59753439639376	36.511120470767764
5	14.962096391788165	36.13511767130417	33.35166123099807	15.551124705909588
6	34.61229161059104	35.104516687070955	16.637497007577494	13.645694694760502
7	30.4413652439448	30.150143419098065	20.809044170435484	18.59944716652165
8	28.358568258386878	32.26316018059121	19.601963091724695	19.77630846929722
9	27.432183060750127	14.051427406080927	18.62529871636248	39.89109081680646
10	16.066639358845457	26.310547852819116	31.346689340433365	26.276123447902055
11	37.79979336936612	21.036190151489134	21.95448416197293	19.209532317171817
12	24.741059465078305	23.647115880389542	28.459623484116797	23.152201170415356
13	29.38431982813802	19.196109934405623	25.153807291638653	26.265762945817713
14	23.54035709675177	19.703793077195524	24.615362972231324	32.14048685382138
15	25.335986138554873	26.91422320633628	21.911997365398683	25.83779328971016
16	26.16535144442762	25.453708231661153	23.392545226020466	24.988395097890763
17	24.371259739727744	25.560281316811604	24.875360862835088	25.19309808062556
18	25.266373337714093	24.53593376222313	25.7952509841196	24.40244191594317
19	25.7786362739171	24.861284044840975	24.971147826166117	24.38893185507581
20	25.758843380353486	24.206607316342524	24.672671211451664	25.361878091852326
21	26.916489596113617	24.074988258515422	23.995746168758757	25.012775976612208
22	25.998214506698496	24.35406241114439	24.475247491969625	25.17247559018749
23	24.61148248096675	24.037246697502805	25.183649095421384	26.167621726109065
24	24.94375215442594	24.962622132415106	24.760763025767865	25.33286268739109
25	24.98202044662121	24.4567443808206	24.964930560440678	25.596304612117514
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	152.0
2	527.5
3	903.0
4	903.0
5	903.0
6	2302.5
7	3702.0
8	3702.0
9	3702.0
10	4191.0
11	4680.0
12	4680.0
13	4680.0
14	5999.0
15	7318.0
16	7318.0
17	7318.0
18	11485.0
19	15652.0
20	15652.0
21	15652.0
22	25253.5
23	34855.0
24	34855.0
25	34855.0
26	51546.0
27	68237.0
28	68237.0
29	68237.0
30	84201.5
31	100166.0
32	100166.0
33	100166.0
34	125010.5
35	149855.0
36	149855.0
37	149855.0
38	170484.5
39	191114.0
40	191114.0
41	191114.0
42	217345.5
43	243577.0
44	243577.0
45	243577.0
46	274944.0
47	306311.0
48	306311.0
49	306311.0
50	311889.5
51	317468.0
52	317468.0
53	317468.0
54	297220.5
55	276973.0
56	276973.0
57	276973.0
58	255952.0
59	234931.0
60	234931.0
61	234931.0
62	210069.0
63	185207.0
64	185207.0
65	185207.0
66	156316.5
67	127426.0
68	127426.0
69	127426.0
70	97608.0
71	67790.0
72	67790.0
73	67790.0
74	53699.5
75	39609.0
76	39609.0
77	39609.0
78	32190.0
79	24771.0
80	24771.0
81	24771.0
82	17779.0
83	10787.0
84	10787.0
85	10787.0
86	7926.0
87	5065.0
88	5065.0
89	5065.0
90	3407.5
91	1750.0
92	1750.0
93	1750.0
94	1072.5
95	395.0
96	395.0
97	395.0
98	715.5
99	1036.0
100	1036.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009505192728114294
2	0.0040087117157699415
3	0.004752596364057147
4	0.015001673740458648
5	0.03434267459592599
6	0.04632748281833097
7	0.06785881069375509
8	0.08740644617374665
9	0.09517590805585747
10	0.1173684667297591
11	0.11807102445314147
12	0.13026246730007066
13	0.1201373706983837
14	0.12009604377347885
15	0.1101775817963161
16	0.1094336971480289
17	0.12203840924400658
18	0.12823744797973327
19	0.1281547941299236
20	0.11960012067462072
21	0.12749356333144607
22	0.14840498733329752
23	0.13642017911089252
24	0.1319568712211693
25	0.12794815950539937
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2419730.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.743546499777906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41653458197099	41.61036696493213
2	12.019158944185598	12.43827819433378
3	3.262826320892773	5.064906163674434
4	1.350410656563601	2.795001464067772
5	0.7352951428778733	1.9023389208281036
6	0.4209146012298215	1.3067768544702445
7	0.31609659348275565	1.144917114830745
8	0.20603706040318695	0.8528870572519881
9	0.16537761208737442	0.7701501744958762
>10	0.9032441194494666	8.604640607754916
>50	0.09427392532554546	3.396472832408222
>100	0.0890393741873665	9.845546213061233
>500	0.014633712784287942	5.259851866631737
>1k	0.006157354559509021	5.007865571258894
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3050	0.12604712095977652	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2873	0.11873225525161898	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2702	0.11166535109289052	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2606	0.10769796630202544	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2585	0.1068301008790237	No Hit
GAACTACGACGGTATCTGATCGTCT	2565	0.10600356238092679	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2556	0.1056316200567832	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2468	0.10199485066515684	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2451	0.10129229294177448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	8.26538498096895E-5
6	0.0	0.0	0.0	0.0	1.65307699619379E-4
7	0.0	0.0	0.0	4.132692490484475E-5	1.65307699619379E-4
8	0.0	0.0	0.0	4.132692490484475E-5	1.65307699619379E-4
9	0.0	0.0	0.0	1.65307699619379E-4	1.65307699619379E-4
10	0.0	0.0	0.0	2.0663462452422379E-4	1.65307699619379E-4
11	0.0	0.0	0.0	2.0663462452422379E-4	2.0663462452422379E-4
12	0.0	0.0	0.0	2.4796154942906853E-4	4.545961739532923E-4
13	0.0	0.0	0.0	2.892884743339133E-4	4.545961739532923E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	35	0.0021729118	16.283195	4
GCGTTAT	230	0.0	13.621562	1
CGTTATT	225	0.0	13.082263	2
AAGACGG	455	0.0	12.734557	5
CAAGACG	470	0.0	12.732074	4
AACCGCG	215	0.0	12.372229	7
AATCGTA	70	1.089587E-4	12.216441	13
CGCATCG	440	0.0	12.093043	13
CGCAAGA	470	0.0	11.919496	2
CGTCGTA	350	0.0	11.672039	10
ATCGCCA	430	0.0	11.491352	16
CGAACGA	225	0.0	11.402957	16
TAACGAA	225	0.0	11.402012	13
GTATTAG	545	0.0	11.3229	1
TATACCG	60	0.005883121	11.081849	5
GCAAGAC	600	0.0	11.0795555	3
ATTAGAC	60	0.005892188	11.0795555	3
TCGCCAG	455	0.0	11.068804	17
CCGTCGT	345	0.0	11.015069	9
AGAACCG	320	0.0	10.982904	5
>>END_MODULE