FastQCFastQC Report
Thu 2 Feb 2017
SRR4062531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419730
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC28450.11757510135428334No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA28150.11633529360713799No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27500.11364904348832308No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT27000.11158269724308086No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA25300.10455712000925724No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA25190.10410252383530394No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG24650.10187086989044232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29950.021.5277141
TAGGACG4050.020.6422464
TAGATCG1301.6261765E-920.3075395
TAGACCG750.001291442517.5998675
TATGGCG5950.017.37802116
CGTCGTA6000.017.23320410
ATACCGT6200.016.6772946
ACCGTCG6600.016.666548
CGACGGT9550.016.5862627
GCGAGAA4000.016.49987620
CCGTCGT6400.016.4998769
TGTAGGA8050.016.398072
TGGCGAG6200.016.32245818
TACGACG9500.016.2104035
GACGGTA9800.016.1631438
ACGACGG10000.016.059886
GGTATCA17050.016.0038491
ACGGTAT9700.015.8761689
CTACGAC9850.015.8580764
TTAACGG3350.015.76107735
Produced by FastQC (version 0.11.5)