##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062531_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2419730 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.328431684526787 32.0 32.0 32.0 32.0 32.0 2 31.413851545420357 32.0 32.0 32.0 32.0 32.0 3 31.488818587197745 32.0 32.0 32.0 32.0 32.0 4 31.59174453348101 32.0 32.0 32.0 32.0 32.0 5 31.52871560050088 32.0 32.0 32.0 32.0 32.0 6 35.11864174928608 36.0 36.0 36.0 36.0 36.0 7 35.13113942464655 36.0 36.0 36.0 36.0 36.0 8 35.072241944349166 36.0 36.0 36.0 36.0 36.0 9 35.20701028627161 36.0 36.0 36.0 36.0 36.0 10 35.07661515954259 36.0 36.0 36.0 36.0 36.0 11 35.21701966748356 36.0 36.0 36.0 36.0 36.0 12 35.11981254106863 36.0 36.0 36.0 36.0 36.0 13 35.17159972393614 36.0 36.0 36.0 36.0 36.0 14 35.13464766730172 36.0 36.0 36.0 36.0 36.0 15 35.085873217259774 36.0 36.0 36.0 36.0 36.0 16 35.104433139234544 36.0 36.0 36.0 36.0 36.0 17 35.07356399267687 36.0 36.0 36.0 36.0 36.0 18 35.08637740574362 36.0 36.0 36.0 36.0 36.0 19 35.07753633669872 36.0 36.0 36.0 36.0 36.0 20 35.07054051485083 36.0 36.0 36.0 36.0 36.0 21 35.0700954238696 36.0 36.0 36.0 36.0 36.0 22 35.05309848619474 36.0 36.0 36.0 36.0 36.0 23 35.01654027515467 36.0 36.0 36.0 36.0 36.0 24 34.98954098184508 36.0 36.0 36.0 36.0 36.0 25 34.96505064614647 36.0 36.0 36.0 32.0 36.0 26 34.92302736255698 36.0 36.0 36.0 32.0 36.0 27 34.906898290305115 36.0 36.0 36.0 32.0 36.0 28 34.89543709422126 36.0 36.0 36.0 32.0 36.0 29 34.87112653064598 36.0 36.0 36.0 32.0 36.0 30 34.861460989449235 36.0 36.0 36.0 32.0 36.0 31 34.8573514400367 36.0 36.0 36.0 32.0 36.0 32 34.82000925723118 36.0 36.0 36.0 32.0 36.0 33 34.80895843751162 36.0 36.0 36.0 32.0 36.0 34 34.79998718865328 36.0 36.0 36.0 32.0 36.0 35 34.78115864166663 36.0 36.0 36.0 32.0 36.0 36 34.75479454319284 36.0 36.0 36.0 32.0 36.0 37 34.744097068681214 36.0 36.0 36.0 32.0 36.0 38 34.71427225351589 36.0 36.0 36.0 32.0 36.0 39 34.71241708785691 36.0 36.0 36.0 32.0 36.0 40 34.70711525666087 36.0 36.0 36.0 32.0 36.0 41 34.679881639687075 36.0 36.0 36.0 32.0 36.0 42 34.656193459600864 36.0 36.0 36.0 32.0 36.0 43 34.65423952259136 36.0 36.0 36.0 32.0 36.0 44 34.62386877874804 36.0 36.0 36.0 32.0 36.0 45 34.59522674017349 36.0 36.0 36.0 32.0 36.0 46 34.595897062895446 36.0 36.0 36.0 32.0 36.0 47 34.581547941299235 36.0 36.0 36.0 32.0 36.0 48 34.54132031259686 36.0 36.0 36.0 32.0 36.0 49 34.53257346894075 36.0 36.0 36.0 32.0 36.0 50 34.02041054167201 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 13.0 21 51.0 22 182.0 23 515.0 24 1402.0 25 3280.0 26 6714.0 27 12871.0 28 22002.0 29 34220.0 30 51637.0 31 74867.0 32 112751.0 33 189639.0 34 428264.0 35 1481319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.05127656135881 17.922203103228924 11.323656633535192 26.70286370187707 2 16.7416432920063 20.432063738328342 36.00824584453161 26.81804712513375 3 18.498658520256093 23.88850592383569 28.437056249576397 29.17577930633182 4 12.548145907308589 15.645642816052053 35.91866523840696 35.8875460382324 5 14.69300293834436 36.37405826269873 33.346199782620374 15.586739016336534 6 34.695200019175786 35.39292595252835 16.41195378965648 13.49992023863938 7 30.49484859881061 30.25147433804598 20.75450566798775 18.49917139515566 8 28.456079172502374 32.52835338172167 19.390394544182428 19.625172901593526 9 27.672963512458004 13.90617961508102 18.442181565711877 39.9786753067491 10 16.041913767238494 26.433899649960946 31.365606906555687 26.15857967624487 11 38.090034838597695 20.97944812024482 21.93918329731003 18.991333743847456 12 24.799213135349813 23.647803680575933 28.33502084943361 23.21796233464064 13 29.573423481132195 19.120397730325283 25.168799824773842 26.137378963768686 14 23.648423584449503 19.627520425832635 24.651882648064042 32.07217334165382 15 25.462634260847285 26.982597231922572 21.767469924330403 25.787298582899744 16 26.273096585156196 25.482099242477467 23.35760601389411 24.887198158472227 17 24.535092758282946 25.589838535704395 24.754456075677865 25.120612630334787 18 25.44552491393668 24.46182012042666 25.77403264000529 24.318622325631374 19 26.03955813251892 24.841118637203323 24.8127683667186 24.306554863559157 20 25.94107607047067 24.195964012513794 24.538150950725907 25.324808966289623 21 27.143730912126557 24.005281581002837 23.949738193930727 24.901249312939875 22 26.173540023060426 24.25907022684349 24.45438127394379 25.1130084761523 23 24.78768292330136 23.94915961698206 25.097180263913742 26.165977195802835 24 25.142061304360404 24.852359560777444 24.716765920164647 25.28881321469751 25 25.265835444450413 24.34333582672447 24.853640695449492 25.537188033375624 26 24.93021948729817 25.07031776272559 25.276167175676623 24.723295574299613 27 25.752377331355152 24.575345183140268 24.34478226909614 25.32749521640844 28 24.809586193500927 24.49302194872982 25.326503370210723 25.37088848755853 29 24.906704467027314 24.682299264794004 25.016510106499485 25.394486161679197 30 24.80090753927091 24.846739098990383 25.411636835514706 24.940716526223998 31 25.445690221636298 24.58766060676191 24.325565249015384 25.641083922586404 32 25.117430457117116 24.63241766643386 24.357634942741548 25.892516933707483 33 24.806693308757588 24.163646357238207 25.03667764585305 25.992982688151155 34 25.458046972182846 24.367098808544753 25.252404193856336 24.922450025416058 35 26.054725113958995 24.27994032392044 24.956048815363697 24.70928574675687 36 24.855459080145305 24.76693680699913 24.812107135920122 25.565496976935442 37 26.01170378513305 24.548193393477785 24.374537655027627 25.065565166361537 38 24.90728304397598 24.38069536683845 25.135944919474483 25.576076669711085 39 25.516235282448868 24.261591169262687 24.46975489000839 25.752418658280057 40 25.797588987201053 24.35656044269402 25.08350105177024 24.762349518334688 41 24.769209787868895 24.736685497968782 25.380063064887402 25.11404164927492 42 26.00538076562261 24.921747467692676 24.830786905977114 24.2420848607076 43 25.17127944026813 23.841626958379656 25.172725882639796 25.81436771871242 44 24.81743830923285 24.562451182569955 24.846325829741332 25.773784678455865 45 24.881288408210832 24.759167345117017 25.03014799171808 25.329396254954066 46 24.739764138779236 24.343521255281587 25.14745458993738 25.769260016001798 47 25.029455777899095 24.102120526720142 25.531578123004685 25.33684557237608 48 25.658069288722295 24.92939294880007 24.571915048373167 24.840622714104466 49 24.634153397279864 24.907696313225028 24.96266112334847 25.495489166146633 50 24.513809391956954 25.193348018167317 24.854508560872492 25.438334029003233 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 12.0 2 19.0 3 73.5 4 128.0 5 138.5 6 149.0 7 165.0 8 181.0 9 263.0 10 345.0 11 521.5 12 698.0 13 1253.5 14 1809.0 15 2417.5 16 3026.0 17 3805.5 18 4585.0 19 5078.0 20 5571.0 21 6977.5 22 8384.0 23 9212.5 24 10041.0 25 12930.5 26 15820.0 27 20088.5 28 24357.0 29 28050.0 30 31743.0 31 34409.0 32 37075.0 33 42666.0 34 48257.0 35 56379.5 36 64502.0 37 77399.5 38 90297.0 39 97182.0 40 104067.0 41 114534.0 42 125001.0 43 126774.5 44 128548.0 45 140968.5 46 153389.0 47 163492.0 48 173595.0 49 178922.0 50 184249.0 51 177580.5 52 170912.0 53 167601.5 54 164291.0 55 167199.0 56 170107.0 57 167047.5 58 163988.0 59 150583.0 60 137178.0 61 120961.0 62 104744.0 63 92365.0 64 79986.0 65 68679.5 66 57373.0 67 51644.5 68 45916.0 69 42433.0 70 38950.0 71 30828.5 72 22707.0 73 20507.0 74 18307.0 75 13688.0 76 9069.0 77 8099.5 78 7130.0 79 6202.0 80 5274.0 81 4194.0 82 3114.0 83 2681.5 84 2249.0 85 1873.0 86 1497.0 87 1127.0 88 757.0 89 484.5 90 212.0 91 144.0 92 76.0 93 50.0 94 24.0 95 19.0 96 14.0 97 11.5 98 9.0 99 7.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01566290453893616 2 0.0035954424667214934 3 5.785769486678266E-4 4 8.26538498096895E-5 5 0.0 6 4.959230988581371E-4 7 0.0 8 4.132692490484475E-5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 8.26538498096895E-5 47 4.132692490484475E-5 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2419730.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.135689471769446 #Duplication Level Percentage of deduplicated Percentage of total 1 80.67509552540396 43.67401919468605 2 12.102761431347925 13.103826691967186 3 3.16139263367406 5.134325097447549 4 1.3130375146169093 2.843287646243397 5 0.7015025708331502 1.8988162669135684 6 0.42317652653599164 1.3745371819376666 7 0.2768277934101951 1.0490384422846626 8 0.18702964488413906 0.8099983021970453 9 0.14520630814858349 0.7074759246546403 >10 0.8379203497588044 8.388620047377163 >50 0.08013132747355392 3.0487873859567105 >100 0.07826351127450576 8.982310006672684 >500 0.013126006570131389 4.979998677574599 >1k 0.004528856068056882 4.004959134087159 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC 2845 0.11757510135428334 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA 2815 0.11633529360713799 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT 2750 0.11364904348832308 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT 2700 0.11158269724308086 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA 2530 0.10455712000925724 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA 2519 0.10410252383530394 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG 2465 0.10187086989044232 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2398077471453427E-4 2 0.0 0.0 0.0 0.0 1.2398077471453427E-4 3 0.0 0.0 0.0 0.0 1.2398077471453427E-4 4 0.0 0.0 0.0 0.0 1.2398077471453427E-4 5 0.0 0.0 0.0 0.0 1.2398077471453427E-4 6 0.0 0.0 0.0 0.0 1.2398077471453427E-4 7 0.0 0.0 0.0 0.0 1.2398077471453427E-4 8 0.0 0.0 0.0 0.0 1.2398077471453427E-4 9 0.0 0.0 0.0 0.0 1.2398077471453427E-4 10 0.0 0.0 0.0 4.132692490484475E-5 1.2398077471453427E-4 11 0.0 0.0 0.0 4.132692490484475E-5 1.2398077471453427E-4 12 0.0 0.0 0.0 8.26538498096895E-5 2.0663462452422379E-4 13 0.0 0.0 0.0 8.26538498096895E-5 2.4796154942906853E-4 14 0.0 0.0 0.0 8.26538498096895E-5 2.4796154942906853E-4 15 0.0 0.0 0.0 8.26538498096895E-5 4.545961739532923E-4 16 0.0 0.0 0.0 8.26538498096895E-5 4.959230988581371E-4 17 0.0 0.0 0.0 8.26538498096895E-5 4.959230988581371E-4 18 0.0 0.0 0.0 1.2398077471453427E-4 5.372500237629818E-4 19 0.0 0.0 0.0 1.65307699619379E-4 5.372500237629818E-4 20 0.0 0.0 0.0 1.65307699619379E-4 6.61230798477516E-4 21 0.0 0.0 0.0 2.4796154942906853E-4 6.61230798477516E-4 22 0.0 0.0 0.0 3.719423241436028E-4 6.61230798477516E-4 23 0.0 0.0 0.0 7.025577233823609E-4 6.61230798477516E-4 24 0.0 0.0 0.0 0.0010331731226211188 6.61230798477516E-4 25 0.0 0.0 0.0 0.001322461596955032 6.61230798477516E-4 26 0.0 0.0 0.0 0.001529096221479256 8.265384980968951E-4 27 0.0 0.0 0.0 0.002355634719576151 8.265384980968951E-4 28 0.0 0.0 0.0 0.0062403656606315585 8.265384980968951E-4 29 0.0 0.0 0.0 0.014836366040839268 8.265384980968951E-4 30 0.0 0.0 0.0 0.03227632835068375 8.265384980968951E-4 31 0.0 0.0 0.0 0.07083434928690391 9.091923479065846E-4 32 0.0 0.0 0.0 0.11621131283242345 9.091923479065846E-4 33 0.0 0.0 0.0 0.16431585342166274 9.091923479065846E-4 34 0.0 0.0 0.0 0.21845412504700937 0.0010331731226211188 35 0.0 0.0 0.0 0.2766424353130308 0.0010331731226211188 36 0.0 0.0 0.0 0.36471011228525496 0.0010331731226211188 37 0.0 0.0 0.0 0.4879056754265972 0.0010745000475259636 38 0.0 0.0 0.0 0.6710252796799643 0.0010745000475259636 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2995 0.0 21.527714 1 TAGGACG 405 0.0 20.642246 4 TAGATCG 130 1.6261765E-9 20.307539 5 TAGACCG 75 0.0012914425 17.599867 5 TATGGCG 595 0.0 17.378021 16 CGTCGTA 600 0.0 17.233204 10 ATACCGT 620 0.0 16.677294 6 ACCGTCG 660 0.0 16.66654 8 CGACGGT 955 0.0 16.586262 7 GCGAGAA 400 0.0 16.499876 20 CCGTCGT 640 0.0 16.499876 9 TGTAGGA 805 0.0 16.39807 2 TGGCGAG 620 0.0 16.322458 18 TACGACG 950 0.0 16.210403 5 GACGGTA 980 0.0 16.163143 8 ACGACGG 1000 0.0 16.05988 6 GGTATCA 1705 0.0 16.003849 1 ACGGTAT 970 0.0 15.876168 9 CTACGAC 985 0.0 15.858076 4 TTAACGG 335 0.0 15.761077 35 >>END_MODULE