FastQCFastQC Report
Thu 2 Feb 2017
SRR4062530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669561
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36920.2211359752653542No Hit
TATCAACGCAGAGTACTTTTTTTTT24310.14560713864303249No Hit
GCGCAAGACGGACCAGAGCGAAAGC22320.13368783770104836No Hit
GTCTTGCGCCGGTCCAAGAATTTCA22020.1318909581620558No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21280.12745865529920739No Hit
GGGTAGGCACACGCTGAGCCAGTCA20570.12320604039025827No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19760.1183544656349783No Hit
GAATAACGCCGCCGCATCGCCAGTC19170.11482060254162621No Hit
GAATAGGACCGCGGTTCTATTTTGT19010.11386226678749684No Hit
GATTAAGAGGGACGGCCGGGGGCAT18790.11254455512556893No Hit
GTATCTGATCGTCTTCGAACCTCCG18440.11044819566341092No Hit
GAACTACGACGGTATCTGATCGTCT18420.11032840369414475No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC17470.10463828515400156No Hit
GTCCTATTCCATTATTCCTAGCTGC17130.10260182167647663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1700.013.4036861
TCGCGTA1750.013.028929
CGTTATT1850.012.8327952
TCGGCGT2350.012.53187613
GGTCGGC2550.012.2940811
GGACCGT701.09071945E-412.2146146
GTATTAC951.0475087E-611.9927721
CGCATCG3250.011.98457713
GGCGTCC2650.011.83086115
CGGCGTC2500.011.78031614
AACGCCA2600.011.69297115
CGCGTAA1950.011.69262110
GCGTAAC1950.011.69227111
AAGACGG4100.011.5842885
CGAACGA1657.2759576E-1211.51753216
AACCGCG1900.011.5003087
GTCGGCG2650.011.4723512
CCGCATC3400.011.45653312
ATCGCCA3650.011.45442316
CGGTCGG2750.011.40030610
Produced by FastQC (version 0.11.5)