##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062530_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1669561
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163902966109056	32.0	32.0	32.0	32.0	32.0
2	30.794633439568845	32.0	32.0	32.0	32.0	32.0
3	30.780957988357418	32.0	32.0	32.0	32.0	32.0
4	30.86213980800941	32.0	32.0	32.0	32.0	32.0
5	30.63228597218071	32.0	32.0	32.0	32.0	32.0
6	34.29862640538441	36.0	36.0	36.0	32.0	36.0
7	34.17228900291753	36.0	36.0	36.0	32.0	36.0
8	34.15994204464527	36.0	36.0	36.0	32.0	36.0
9	34.3432752681693	36.0	36.0	36.0	32.0	36.0
10	33.987493119448764	36.0	36.0	36.0	32.0	36.0
11	34.3224278717579	36.0	36.0	36.0	32.0	36.0
12	34.08744873652415	36.0	36.0	36.0	32.0	36.0
13	34.23165790288585	36.0	36.0	36.0	32.0	36.0
14	34.125059821114654	36.0	36.0	36.0	32.0	36.0
15	34.05376143788696	36.0	36.0	36.0	32.0	36.0
16	34.058466866439744	36.0	36.0	36.0	32.0	36.0
17	33.97124214089811	36.0	36.0	36.0	32.0	36.0
18	33.98668931533499	36.0	36.0	36.0	32.0	36.0
19	33.974390872810275	36.0	36.0	36.0	32.0	36.0
20	33.97006338792054	36.0	36.0	36.0	32.0	36.0
21	33.9546347812389	36.0	36.0	36.0	32.0	36.0
22	33.902103606876295	36.0	36.0	36.0	32.0	36.0
23	33.87030243279521	36.0	36.0	36.0	32.0	36.0
24	33.83830599780421	36.0	36.0	36.0	32.0	36.0
25	33.37977827704408	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	17.0
4	63.0
5	170.0
6	271.0
7	82.0
8	246.0
9	303.0
10	185.0
11	71.0
12	119.0
13	107.0
14	312.0
15	385.0
16	536.0
17	757.0
18	1033.0
19	1452.0
20	2242.0
21	3339.0
22	5155.0
23	7704.0
24	10599.0
25	15178.0
26	20780.0
27	26749.0
28	35979.0
29	47147.0
30	60965.0
31	83076.0
32	118257.0
33	170063.0
34	376431.0
35	679788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.87569118854779	17.186042708578555	10.901535695010264	26.03673040786339
2	17.368045584987573	19.738160655574703	36.05061883908746	26.84317492035026
3	19.39819043825417	23.474226773247718	27.73726912513889	29.39031366335922
4	12.89134129399847	14.638849667313847	34.94411699338222	37.52569204530546
5	15.150886926927768	36.096323170348086	32.835080424095175	15.917709478628971
6	35.3263777821855	34.35537309434937	16.43445209005104	13.88379703341409
7	30.97880583614186	29.902105630979047	20.420622728779314	18.69846580409978
8	28.94635560543278	31.812497602056478	19.294692871393494	19.94645392111725
9	27.565696347408387	14.03228775153048	18.402291690523455	39.99972421053767
10	16.465275820505287	26.019537410723387	30.70689686190085	26.808289906870474
11	38.20368510217238	20.96886103656617	21.438419214104737	19.389034647156713
12	25.087593876285418	23.34879095860867	27.976542473883768	23.58707269122214
13	29.567464389138724	18.954938984935286	25.17098550618606	26.306611119739927
14	23.83933792110826	19.40005097527699	24.420516049715886	32.34009505389886
15	25.6222888235668	26.633661822678118	21.961833351922543	25.782216001832538
16	26.472110241045854	25.323276314377186	23.15099140068514	25.053622043891828
17	24.486646912745115	25.462289140080728	24.681727382689353	25.3693365644848
18	25.567982132597333	24.174070435661875	25.66735876463511	24.590588667105674
19	25.80964575842722	24.77022127728482	24.95824249639401	24.46189046789395
20	25.979449697094726	23.755185851067274	24.60675087165476	25.658613580183243
21	27.197650945127545	23.80978940790239	24.027854244407738	24.96470540256232
22	26.298172726585623	23.790965488034725	24.539282599332722	25.371579186046926
23	24.77090468507267	23.60650427960485	25.306829973428663	26.315761061893824
24	25.01872744534855	24.6473572560829	24.854573664983544	25.479341633585012
25	25.08847676034418	24.24494133635443	24.813435633119365	25.853146270182027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	197.0
2	540.0
3	883.0
4	883.0
5	883.0
6	2290.5
7	3698.0
8	3698.0
9	3698.0
10	3889.5
11	4081.0
12	4081.0
13	4081.0
14	4289.5
15	4498.0
16	4498.0
17	4498.0
18	7036.5
19	9575.0
20	9575.0
21	9575.0
22	15637.5
23	21700.0
24	21700.0
25	21700.0
26	31930.5
27	42161.0
28	42161.0
29	42161.0
30	52028.0
31	61895.0
32	61895.0
33	61895.0
34	78010.0
35	94125.0
36	94125.0
37	94125.0
38	109248.5
39	124372.0
40	124372.0
41	124372.0
42	144645.0
43	164918.0
44	164918.0
45	164918.0
46	189737.5
47	214557.0
48	214557.0
49	214557.0
50	220242.5
51	225928.0
52	225928.0
53	225928.0
54	210862.5
55	195797.0
56	195797.0
57	195797.0
58	182827.5
59	169858.0
60	169858.0
61	169858.0
62	152192.5
63	134527.0
64	134527.0
65	134527.0
66	112852.0
67	91177.0
68	91177.0
69	91177.0
70	69585.5
71	47994.0
72	47994.0
73	47994.0
74	37767.5
75	27541.0
76	27541.0
77	27541.0
78	22595.5
79	17650.0
80	17650.0
81	17650.0
82	12318.0
83	6986.0
84	6986.0
85	6986.0
86	5123.0
87	3260.0
88	3260.0
89	3260.0
90	2211.5
91	1163.0
92	1163.0
93	1163.0
94	711.5
95	260.0
96	260.0
97	260.0
98	510.0
99	760.0
100	760.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009223981633495271
2	0.0038333430165175156
3	0.005750014524776273
4	0.01617191585093327
5	0.035698006841319366
6	0.046359492106008704
7	0.06768246263538738
8	0.08774761748747126
9	0.09715128707486578
10	0.11925290540447459
11	0.12194822471296347
12	0.1321904380852212
13	0.12110968092810027
14	0.12494302394461777
15	0.11541956238795709
16	0.11200549126387116
17	0.12242739259002815
18	0.12979459869989776
19	0.13153158225425726
20	0.12236749660539507
21	0.12979459869989776
22	0.14896131378248534
23	0.1408154598723856
24	0.13308887785471749
25	0.13021387059232936
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1669561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.649227545735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.88359585893937	38.1721114533481
2	13.671174079089967	13.575264653401838
3	4.144135967844055	6.1725944894386275
4	1.7429613904119408	3.461467467039725
5	0.939461241079215	2.3321762464370277
6	0.5737354603326985	1.7091313452669097
7	0.3702669585219833	1.286842793342762
8	0.2644223181754286	1.0502691074610078
9	0.2007296075076578	0.8969462962482402
>10	1.006793234670138	8.956468224004348
>50	0.09100838254079104	3.199623141209752
>100	0.09128288957095315	9.899242437885292
>500	0.014266557013407094	4.680938075814685
>1k	0.006166054302404761	4.606924269101687
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3692	0.2211359752653542	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2431	0.14560713864303249	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2232	0.13368783770104836	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2202	0.1318909581620558	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2128	0.12745865529920739	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2057	0.12320604039025827	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1976	0.1183544656349783	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1917	0.11482060254162621	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1901	0.11386226678749684	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1879	0.11254455512556893	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1844	0.11044819566341092	No Hit
GAACTACGACGGTATCTGATCGTCT	1842	0.11032840369414475	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1747	0.10463828515400156	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1713	0.10260182167647663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1979196926617236E-4
2	0.0	0.0	0.0	0.0	1.1979196926617236E-4
3	0.0	0.0	0.0	0.0	1.1979196926617236E-4
4	0.0	0.0	0.0	0.0	1.1979196926617236E-4
5	0.0	0.0	0.0	0.0	1.1979196926617236E-4
6	0.0	0.0	0.0	0.0	1.7968795389925854E-4
7	0.0	0.0	0.0	1.1979196926617236E-4	1.7968795389925854E-4
8	0.0	0.0	0.0	1.7968795389925854E-4	1.7968795389925854E-4
9	0.0	0.0	0.0	1.7968795389925854E-4	1.7968795389925854E-4
10	0.0	0.0	0.0	1.7968795389925854E-4	1.7968795389925854E-4
11	0.0	0.0	0.0	1.7968795389925854E-4	1.7968795389925854E-4
12	0.0	0.0	0.0	1.7968795389925854E-4	3.5937590779851707E-4
13	0.0	0.0	0.0	1.7968795389925854E-4	4.7916787706468945E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	170	0.0	13.403686	1
TCGCGTA	175	0.0	13.02892	9
CGTTATT	185	0.0	12.832795	2
TCGGCGT	235	0.0	12.531876	13
GGTCGGC	255	0.0	12.29408	11
GGACCGT	70	1.09071945E-4	12.214614	6
GTATTAC	95	1.0475087E-6	11.992772	1
CGCATCG	325	0.0	11.984577	13
GGCGTCC	265	0.0	11.830861	15
CGGCGTC	250	0.0	11.780316	14
AACGCCA	260	0.0	11.692971	15
CGCGTAA	195	0.0	11.692621	10
GCGTAAC	195	0.0	11.692271	11
AAGACGG	410	0.0	11.584288	5
CGAACGA	165	7.2759576E-12	11.517532	16
AACCGCG	190	0.0	11.500308	7
GTCGGCG	265	0.0	11.47235	12
CCGCATC	340	0.0	11.456533	12
ATCGCCA	365	0.0	11.454423	16
CGGTCGG	275	0.0	11.400306	10
>>END_MODULE