FastQCFastQC Report
Thu 2 Feb 2017
SRR4062530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669561
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC23150.1386592044255945No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21660.1297347027152647No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20680.12386489622122222No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19860.11895342548130916No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19530.11697685798841731No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18920.11332320292579906No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG18780.11248465914093585No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA18580.11128673944827412No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16740.10026587827578627No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC16720.1001460863065201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT3700.019.6213954
TTAACGG2600.018.6151735
TAACGGC2800.018.07121836
GTATCAA37800.017.9903871
TGCGTAC1355.5300916E-817.92571816
GTCTATA1601.0968506E-917.881221
TATTAGA3850.017.7167342
CTCTATA1509.586074E-917.6024322
GGCGTTA4800.017.41646442
CAAGACG8900.016.8087944
GTATTAG5000.016.7258191
CTATAGA2500.016.7258191
GTATAGG1854.2018655E-1016.6544421
CAGAGCG8650.016.531614
ATAATAC1753.6288839E-916.3426693
ACGGACC8800.016.2498118
GCGAAAG8700.016.1837218
CGCAATA5750.016.06937836
GTGACTA3300.015.99981624
AACGCAG42550.015.8212036
Produced by FastQC (version 0.11.5)