##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062530_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1669561
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272387172436346	32.0	32.0	32.0	32.0	32.0
2	31.404657871140977	32.0	32.0	32.0	32.0	32.0
3	31.49384538809903	32.0	32.0	32.0	32.0	32.0
4	31.600340448776656	32.0	32.0	32.0	32.0	32.0
5	31.53037834496613	32.0	32.0	32.0	32.0	32.0
6	35.129401681040704	36.0	36.0	36.0	36.0	36.0
7	35.13663052742607	36.0	36.0	36.0	36.0	36.0
8	35.07467951156023	36.0	36.0	36.0	36.0	36.0
9	35.207281435059876	36.0	36.0	36.0	36.0	36.0
10	35.07689087131288	36.0	36.0	36.0	36.0	36.0
11	35.21250735971911	36.0	36.0	36.0	36.0	36.0
12	35.1219991362999	36.0	36.0	36.0	36.0	36.0
13	35.16806274224182	36.0	36.0	36.0	36.0	36.0
14	35.124753752633175	36.0	36.0	36.0	36.0	36.0
15	35.08810699339527	36.0	36.0	36.0	36.0	36.0
16	35.1001005653582	36.0	36.0	36.0	36.0	36.0
17	35.06888517400682	36.0	36.0	36.0	36.0	36.0
18	35.08821420720776	36.0	36.0	36.0	36.0	36.0
19	35.0690349139684	36.0	36.0	36.0	36.0	36.0
20	35.06221036547931	36.0	36.0	36.0	36.0	36.0
21	35.05721024868214	36.0	36.0	36.0	36.0	36.0
22	35.043466516048234	36.0	36.0	36.0	36.0	36.0
23	35.0058769940122	36.0	36.0	36.0	36.0	36.0
24	34.97800918924196	36.0	36.0	36.0	36.0	36.0
25	34.955538012687164	36.0	36.0	36.0	32.0	36.0
26	34.90837771126662	36.0	36.0	36.0	32.0	36.0
27	34.89722807372716	36.0	36.0	36.0	32.0	36.0
28	34.883575382989896	36.0	36.0	36.0	32.0	36.0
29	34.85724630606489	36.0	36.0	36.0	32.0	36.0
30	34.847995371238305	36.0	36.0	36.0	32.0	36.0
31	34.84987191243686	36.0	36.0	36.0	32.0	36.0
32	34.810670589454354	36.0	36.0	36.0	32.0	36.0
33	34.79450346528219	36.0	36.0	36.0	32.0	36.0
34	34.77603933009935	36.0	36.0	36.0	32.0	36.0
35	34.74696162643953	36.0	36.0	36.0	32.0	36.0
36	34.72578300523311	36.0	36.0	36.0	32.0	36.0
37	34.71403320992764	36.0	36.0	36.0	32.0	36.0
38	34.68195771223693	36.0	36.0	36.0	32.0	36.0
39	34.67377711865574	36.0	36.0	36.0	32.0	36.0
40	34.66697592960065	36.0	36.0	36.0	32.0	36.0
41	34.62861195248332	36.0	36.0	36.0	32.0	36.0
42	34.60295970018466	36.0	36.0	36.0	32.0	36.0
43	34.5883426840948	36.0	36.0	36.0	32.0	36.0
44	34.55788377902934	36.0	36.0	36.0	32.0	36.0
45	34.5144831485642	36.0	36.0	36.0	32.0	36.0
46	34.51451070071713	36.0	36.0	36.0	32.0	36.0
47	34.48855297889685	36.0	36.0	36.0	32.0	36.0
48	34.445214041295884	36.0	36.0	36.0	32.0	36.0
49	34.43718797935505	36.0	36.0	36.0	32.0	36.0
50	33.919152998902106	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	11.0
21	35.0
22	164.0
23	417.0
24	1146.0
25	2569.0
26	5354.0
27	9878.0
28	16264.0
29	25411.0
30	36488.0
31	52866.0
32	78376.0
33	129204.0
34	290102.0
35	1021270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.381497799995806	17.809041433162704	11.387245666635716	26.42221510020577
2	16.80837567632484	20.167523110198797	35.799559269506275	27.22454194397008
3	18.802064740565754	23.786144340171877	28.080280026545978	29.331510892716388
4	12.617942822026654	15.102191656599137	35.161021563179254	37.118843958194944
5	14.915178301361856	36.38064137818265	32.70338729761896	16.000793022836543
6	35.52956989086301	34.606628241211865	16.084185407710926	13.7796164602142
7	30.958557369272523	29.93325790432335	20.40302810139911	18.705156625005014
8	29.019245178822455	31.998890726364593	19.070582027251476	19.911282067561473
9	27.59875200726418	13.822915125592896	18.34152810229755	40.23680476484537
10	16.35693454746487	26.09410497729643	30.6886061665312	26.860354308707496
11	38.43926637002182	20.906094476332402	21.386340481120484	19.26829867252529
12	25.106420190696838	23.393814302083	27.84336720850571	23.65639829871445
13	29.655160847671937	18.79428185013905	25.247415338523123	26.3031419636659
14	23.934195875442708	19.18959534871742	24.355624023321102	32.52058475251878
15	25.647640307841403	26.605676582047614	21.86089636736843	25.885786742742557
16	26.49564765827664	25.283951889149304	23.041805600394355	25.178594852179703
17	24.64983309983882	25.32150667151425	24.626234081893383	25.402426146753548
18	25.747187434301594	23.989479869259046	25.67680965235772	24.58652304408165
19	25.91405764748937	24.58310897295756	24.93529736260011	24.56753601695296
20	26.00659694374749	23.688083274585356	24.586702732035548	25.71861704963161
21	27.30975388140954	23.706830717775514	24.020206509375818	24.963208891439127
22	26.298649764818414	23.710364580868863	24.58790065172821	25.403085002584515
23	24.8154454973493	23.44634308060622	25.346782777029407	26.39142864501507
24	25.12702440941062	24.554838068210746	24.793583462958228	25.524554059420414
25	25.22573299208594	23.939047450197986	24.930385891860197	25.904833665855875
26	24.9857896776458	24.856054974930537	25.294194102521562	24.863961244902104
27	25.94029208875866	24.130235433146797	24.50015303424074	25.429319443853803
28	24.933320795107218	23.995050195829922	25.439801241164595	25.63182776789827
29	24.85533622311494	24.425762221326444	25.320728023714018	25.3981735318446
30	24.788492304264416	24.52285361241668	25.666028375123762	25.02262570819515
31	25.332168156778938	24.49027019677628	24.447085191855823	25.730476454588963
32	25.177037556579247	24.328670830236213	24.376827201881213	26.11746441130333
33	24.800711085129564	23.90724268235782	25.015318398069912	26.27672783444271
34	25.521679052158024	24.167071463696146	25.177576620440945	25.13367286370489
35	26.217131329732783	23.878612401703204	25.197581879308395	24.706674389255618
36	24.910919696854442	24.6787628604166	24.668520647044343	25.741796795684614
37	26.097219568497348	24.35071255258119	24.55184326897909	25.00022460994237
38	25.01430016633115	23.944917256692026	25.14882654781706	25.89195602915976
39	25.712507659199034	24.109391630494486	24.47805141591113	25.700049294395356
40	25.779651057972725	24.180847540161754	25.130498376519338	24.909003025346184
41	24.5661584093064	24.819578320288986	25.557436954983974	25.05682631542064
42	26.193113039894918	24.71715618656641	24.79885430960594	24.290876463932733
43	25.19452718409211	23.641544094525447	25.082521692828237	26.081407028554217
44	24.790109495849507	24.451757078657206	24.846112241481443	25.912021184011845
45	24.940987481140255	24.613476237166537	24.93823226584713	25.50730401584608
46	24.743015816162124	24.388670534356997	25.09137148874133	25.776942160739548
47	25.00035937612305	24.039327727065814	25.533973022832363	25.42633987397877
48	25.822177207062214	25.028555410673825	24.270332141203586	24.878935241060375
49	24.66959877476774	24.901456131282416	24.77345841212151	25.65548668182834
50	24.654953793187303	25.175630211331256	24.61596146048148	25.553454534999958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	41.0
2	53.0
3	153.5
4	254.0
5	278.0
6	302.0
7	283.0
8	264.0
9	367.5
10	471.0
11	670.5
12	870.0
13	1567.5
14	2265.0
15	3029.0
16	3793.0
17	4309.0
18	4825.0
19	5116.0
20	5407.0
21	6173.0
22	6939.0
23	7418.0
24	7897.0
25	8846.0
26	9795.0
27	11893.5
28	13992.0
29	15820.0
30	17648.0
31	19703.0
32	21758.0
33	24974.0
34	28190.0
35	32972.0
36	37754.0
37	46185.5
38	54617.0
39	59871.5
40	65126.0
41	72547.0
42	79968.0
43	82364.5
44	84761.0
45	95009.5
46	105258.0
47	112890.0
48	120522.0
49	126495.5
50	132469.0
51	126555.0
52	120641.0
53	120415.5
54	120190.0
55	122215.5
56	124241.0
57	121732.0
58	119223.0
59	109452.5
60	99682.0
61	88039.5
62	76397.0
63	67170.5
64	57944.0
65	49995.5
66	42047.0
67	36979.5
68	31912.0
69	29369.5
70	26827.0
71	20713.0
72	14599.0
73	13211.0
74	11823.0
75	8663.5
76	5504.0
77	5062.5
78	4621.0
79	3996.0
80	3371.0
81	2681.5
82	1992.0
83	1765.5
84	1539.0
85	1292.5
86	1046.0
87	772.5
88	499.0
89	323.0
90	147.0
91	100.5
92	54.0
93	37.5
94	21.0
95	17.0
96	13.0
97	7.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014734412219739201
2	0.0035937590779851706
3	2.9947992316543094E-4
4	1.1979196926617236E-4
5	0.0
6	4.7916787706468945E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.7968795389925854E-4
47	5.989598463308618E-5
48	0.0
49	0.0
50	1.1979196926617236E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1669561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.47461303894254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.66847592413649	40.75623219443487
2	13.370086232499837	14.031802026954443
3	4.046022509776422	6.369403966421065
4	1.6344708616532038	3.4307290395471544
5	0.8769323520148414	2.300834291665428
6	0.5361164490180309	1.6879501923619886
7	0.3451188674945929	1.2676985316951797
8	0.23629530259022505	0.9919603653073517
9	0.1886900891350155	0.8911295470479164
>10	0.9232493782060052	8.668162714117509
>50	0.0763403695362198	2.8417321382007366
>100	0.08206392894485016	9.287814833920443
>500	0.011674106166207919	4.131167146282621
>1k	0.004463628828255969	3.3433830120432537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2315	0.1386592044255945	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2166	0.1297347027152647	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2068	0.12386489622122222	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1986	0.11895342548130916	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1953	0.11697685798841731	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1892	0.11332320292579906	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1878	0.11248465914093585	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1858	0.11128673944827412	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1674	0.10026587827578627	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1672	0.1001460863065201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	5.989598463308618E-5
14	0.0	0.0	0.0	0.0	5.989598463308618E-5
15	0.0	0.0	0.0	0.0	5.989598463308618E-5
16	0.0	0.0	0.0	0.0	1.1979196926617236E-4
17	0.0	0.0	0.0	0.0	1.1979196926617236E-4
18	0.0	0.0	0.0	0.0	1.7968795389925854E-4
19	0.0	0.0	0.0	2.3958393853234472E-4	1.7968795389925854E-4
20	0.0	0.0	0.0	4.1927189243160326E-4	2.9947992316543094E-4
21	0.0	0.0	0.0	5.390638616977756E-4	3.5937590779851707E-4
22	0.0	0.0	0.0	8.984397694962928E-4	3.5937590779851707E-4
23	0.0	0.0	0.0	0.001976567492891844	3.5937590779851707E-4
24	0.0	0.0	0.0	0.003054695216287395	3.5937590779851707E-4
25	0.0	0.0	0.0	0.0037734470318844296	3.5937590779851707E-4
26	0.0	0.0	0.0	0.0050911586938123254	4.1927189243160326E-4
27	0.0	0.0	0.0	0.006768246263538739	4.7916787706468945E-4
28	0.0	0.0	0.0	0.012278676849782667	5.390638616977756E-4
29	0.0	0.0	0.0	0.023599017945435955	5.390638616977756E-4
30	0.0	0.0	0.0	0.04588032422894402	5.390638616977756E-4
31	0.0	0.0	0.0	0.09367731996614678	5.390638616977756E-4
32	0.0	0.0	0.0	0.14938058567491694	5.390638616977756E-4
33	0.0	0.0	0.0	0.20262811601373057	5.390638616977756E-4
34	0.0	0.0	0.0	0.26515952397067255	5.989598463308619E-4
35	0.0	0.0	0.0	0.33260240266752755	5.989598463308619E-4
36	0.0	0.0	0.0	0.4326885929894146	5.989598463308619E-4
37	0.0	0.0	0.0	0.5770978119397854	5.989598463308619E-4
38	0.0	0.0	0.0	0.7636139080872157	6.58855830963948E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACT	370	0.0	19.621395	4
TTAACGG	260	0.0	18.61517	35
TAACGGC	280	0.0	18.071218	36
GTATCAA	3780	0.0	17.990387	1
TGCGTAC	135	5.5300916E-8	17.925718	16
GTCTATA	160	1.0968506E-9	17.88122	1
TATTAGA	385	0.0	17.716734	2
CTCTATA	150	9.586074E-9	17.602432	2
GGCGTTA	480	0.0	17.416464	42
CAAGACG	890	0.0	16.808794	4
GTATTAG	500	0.0	16.725819	1
CTATAGA	250	0.0	16.725819	1
GTATAGG	185	4.2018655E-10	16.654442	1
CAGAGCG	865	0.0	16.5316	14
ATAATAC	175	3.6288839E-9	16.342669	3
ACGGACC	880	0.0	16.249811	8
GCGAAAG	870	0.0	16.18372	18
CGCAATA	575	0.0	16.069378	36
GTGACTA	330	0.0	15.999816	24
AACGCAG	4255	0.0	15.821203	6
>>END_MODULE