FastQCFastQC Report
Thu 2 Feb 2017
SRR4062529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404154
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG111320.792790534371586No Hit
TCCATGTACTCTGCGTTGATACCAC95410.6794838742758985No Hit
GAGTACATGGAAGCAGTGGTATCAA47130.33564694470834394No Hit
CATGTACTCTGCGTTGATACCACTG39870.28394321420584917No Hit
GCTTCCATGTACTCTGCGTTGATAC33880.24128407567830878No Hit
CCCATGTACTCTGCGTTGATACCAC25030.17825680089220983No Hit
CATGGAAGCAGTGGTATCAACGCAG24100.17163359574519604No Hit
GCGTTGATACCACTGCTTCCATGTA24020.17106385766803356No Hit
GTATCAACGCAGAGTACTTTTTTTT22380.15938422708620278No Hit
GTACATGGGAAGCAGTGGTATCAAC21770.1550399742478389No Hit
ACGCAGAGTACATGGAAGCAGTGGT19360.1378766146733193No Hit
GTATCAACGCAGAGTACATGGAAGC19100.13602496592254126No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17060.12149664495489809No Hit
TATCAACGCAGAGTACTTTTTTTTT15970.11373396365355938No Hit
ACTCTGCGTTGATACCACTGCTTCC15600.11109892504668291No Hit
CAGTGGTATCAACGCAGAGTACATG14950.1064698031697378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATA759.705818E-713.92886452
CGTCTTA655.444456E-513.15572415
CCTTATA2100.013.1148392
CCGTCGT1102.744855E-912.9545469
CGAATGA2350.012.93709314
ACCGTTA604.0915835E-412.6666678
CCGTTAT604.0915835E-412.6666679
TAGGACC5400.012.4889594
CTCGTCT701.0903913E-412.21472113
CTTATAC2500.012.1573993
GTCGTGG550.003081322212.0840161
ACTGTTC3000.012.0333348
GCGTTAT802.8826675E-511.868231
GCGAATG2650.011.83061113
GACCGTT658.0225634E-411.6923087
CGACGTT1552.5465852E-1111.6451627
CCCTTAT2450.011.626021
AACCGCG907.458997E-611.6111127
CGTTATT907.4856853E-611.6073882
CGTCGTA1157.07023E-811.56521710
Produced by FastQC (version 0.11.5)