##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062529_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1404154
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.140001737701134	32.0	32.0	32.0	32.0	32.0
2	30.787839510481046	32.0	32.0	32.0	32.0	32.0
3	30.780769773116056	32.0	32.0	32.0	32.0	32.0
4	30.84367740290595	32.0	32.0	32.0	32.0	32.0
5	30.631244863455148	32.0	32.0	32.0	32.0	32.0
6	34.289182667997956	36.0	36.0	36.0	32.0	36.0
7	34.17450222696371	36.0	36.0	36.0	32.0	36.0
8	34.14554956222751	36.0	36.0	36.0	32.0	36.0
9	34.32831868869084	36.0	36.0	36.0	32.0	36.0
10	33.97936835988075	36.0	36.0	36.0	32.0	36.0
11	34.32382274308943	36.0	36.0	36.0	32.0	36.0
12	34.101846378673564	36.0	36.0	36.0	32.0	36.0
13	34.20346913515184	36.0	36.0	36.0	32.0	36.0
14	34.114493139641375	36.0	36.0	36.0	32.0	36.0
15	34.04875960898876	36.0	36.0	36.0	32.0	36.0
16	34.0429091111089	36.0	36.0	36.0	32.0	36.0
17	33.97510387037319	36.0	36.0	36.0	32.0	36.0
18	33.98813876540607	36.0	36.0	36.0	32.0	36.0
19	33.98620877766969	36.0	36.0	36.0	32.0	36.0
20	33.96733976472666	36.0	36.0	36.0	32.0	36.0
21	33.93190632936273	36.0	36.0	36.0	32.0	36.0
22	33.87863012176727	36.0	36.0	36.0	32.0	36.0
23	33.80958427636855	36.0	36.0	36.0	32.0	36.0
24	33.78016371423647	36.0	36.0	36.0	32.0	36.0
25	33.27324282094414	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	47.0
5	143.0
6	237.0
7	50.0
8	195.0
9	255.0
10	168.0
11	51.0
12	112.0
13	84.0
14	208.0
15	290.0
16	451.0
17	659.0
18	901.0
19	1313.0
20	1928.0
21	2872.0
22	4286.0
23	6362.0
24	8898.0
25	12204.0
26	17287.0
27	22346.0
28	30676.0
29	40227.0
30	51994.0
31	71743.0
32	102765.0
33	146013.0
34	315587.0
35	563797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.780734966909684	17.430108629693255	11.182334356099132	25.606822047297932
2	15.699035466872349	19.78713751665659	38.525132487096705	25.98869452937435
3	19.786323629749177	24.523569069131092	28.31400640701495	27.376100894104777
4	12.20342604793618	15.642936001994373	37.12254709925567	35.031090850813776
5	13.453315418187103	37.52363474189509	34.56906046646823	14.453989373449582
6	32.83240626676575	36.22926746897646	17.52772115471621	13.410605109541576
7	28.84243023303048	31.022578992265576	21.324722581914145	18.810268192789803
8	27.70877541375877	34.354148564683506	19.738533113887996	18.19854290766973
9	27.25861283601092	14.579001282417762	18.66819596826105	39.494189913310265
10	15.429057905758489	27.614343783934864	32.817826414631554	24.13877189567509
11	36.79482864835265	21.605551816190562	22.062594117915488	19.537025417541297
12	22.84696382143331	24.359170005177127	30.525799352787736	22.26806682060183
13	29.967821540387778	20.44968559513038	25.433175685262867	24.149317179218976
14	22.925644938180053	20.193588408938577	25.43609086890179	31.44467578397958
15	25.66006177117288	27.58855721818685	23.377865044531713	23.37351596610856
16	25.281650275982336	26.465928907634677	25.078745153733067	23.173675662649913
17	23.41500074511026	26.41897157671748	26.62724593701002	23.538781741162243
18	24.238716228550565	25.32067189211391	28.148674242424242	22.291937636911282
19	24.97279543162918	26.82855345858131	25.191858168287528	23.006792941501978
20	24.380644080244426	24.958911630593278	27.346992616571537	23.31345167259076
21	25.45427837782527	25.300825947722068	24.47716004617834	24.767735628274323
22	23.84085507978215	26.551850552184348	25.29570605705287	24.311588310980635
23	23.1790502257445	26.100286040496375	25.97491600992406	24.74574772383507
24	23.55517555388057	26.96021558360247	25.468629776237172	24.015979086279792
25	24.56701930728794	25.568536736329587	25.027239562683796	24.837204393698677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	223.0
1	223.0
2	492.5
3	762.0
4	762.0
5	762.0
6	1876.0
7	2990.0
8	2990.0
9	2990.0
10	3299.0
11	3608.0
12	3608.0
13	3608.0
14	4079.5
15	4551.0
16	4551.0
17	4551.0
18	7630.5
19	10710.0
20	10710.0
21	10710.0
22	16755.0
23	22800.0
24	22800.0
25	22800.0
26	33116.5
27	43433.0
28	43433.0
29	43433.0
30	55743.0
31	68053.0
32	68053.0
33	68053.0
34	79601.0
35	91149.0
36	91149.0
37	91149.0
38	104851.5
39	118554.0
40	118554.0
41	118554.0
42	141682.5
43	164811.0
44	164811.0
45	164811.0
46	196108.5
47	227406.0
48	227406.0
49	227406.0
50	210349.0
51	193292.0
52	193292.0
53	193292.0
54	172238.0
55	151184.0
56	151184.0
57	151184.0
58	134704.0
59	118224.0
60	118224.0
61	118224.0
62	101473.5
63	84723.0
64	84723.0
65	84723.0
66	67625.0
67	50527.0
68	50527.0
69	50527.0
70	36946.0
71	23365.0
72	23365.0
73	23365.0
74	17630.0
75	11895.0
76	11895.0
77	11895.0
78	9453.5
79	7012.0
80	7012.0
81	7012.0
82	4807.0
83	2602.0
84	2602.0
85	2602.0
86	1869.5
87	1137.0
88	1137.0
89	1137.0
90	776.5
91	416.0
92	416.0
93	416.0
94	263.0
95	110.0
96	110.0
97	110.0
98	363.5
99	617.0
100	617.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008403636638146528
2	0.003916949280492026
3	0.00484277365588105
4	0.01452832096764315
5	0.03560862982265478
6	0.044368352759027854
7	0.06744274488410815
8	0.08709870854621359
9	0.09507504162648826
10	0.1175796956744061
11	0.11807821649192325
12	0.1304700196702071
13	0.1184343027901498
14	0.12064203783915439
15	0.1109564905273923
16	0.108819972738033
17	0.11943134442518413
18	0.12719402572652286
19	0.12933054351588216
20	0.12028595154092786
21	0.12569846327397138
22	0.14855920362011577
23	0.1356688796243147
24	0.12968662981410872
25	0.12690915668794164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1404154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.52700474477949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.72552919083228	41.08017720896071
2	12.182492774386242	12.554547259780835
3	3.5075215658585153	5.4219624109922435
4	1.3482152989065797	2.7787798441497458
5	0.7302775259247655	1.8814506771665611
6	0.42586018992261615	1.3165980016053203
7	0.3025460780907882	1.091250524090893
8	0.22783843685101174	0.9391865773332181
9	0.17317481529428852	0.8030861576410607
>10	1.1344269698324683	11.45461646126249
>50	0.1403484930786606	5.041141743964553
>100	0.09113614060597663	8.674162607393214
>500	0.00773274212064124	2.657913020002675
>1k	0.0026236089337889922	2.8342249501763184
>5k	1.3808468072573643E-4	0.6788507367985203
>10k+	1.3808468072573643E-4	0.7920518186815981
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	11132	0.792790534371586	No Hit
TCCATGTACTCTGCGTTGATACCAC	9541	0.6794838742758985	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4713	0.33564694470834394	No Hit
CATGTACTCTGCGTTGATACCACTG	3987	0.28394321420584917	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3388	0.24128407567830878	No Hit
CCCATGTACTCTGCGTTGATACCAC	2503	0.17825680089220983	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2410	0.17163359574519604	No Hit
GCGTTGATACCACTGCTTCCATGTA	2402	0.17106385766803356	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2238	0.15938422708620278	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2177	0.1550399742478389	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1936	0.1378766146733193	No Hit
GTATCAACGCAGAGTACATGGAAGC	1910	0.13602496592254126	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1706	0.12149664495489809	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1597	0.11373396365355938	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1560	0.11109892504668291	No Hit
CAGTGGTATCAACGCAGAGTACATG	1495	0.1064698031697378	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4243451929061912E-4
2	0.0	0.0	0.0	0.0	1.4243451929061912E-4
3	0.0	0.0	0.0	0.0	1.4243451929061912E-4
4	0.0	0.0	0.0	0.0	1.4243451929061912E-4
5	0.0	0.0	0.0	0.0	1.4243451929061912E-4
6	0.0	0.0	0.0	0.0	2.8486903858123823E-4
7	0.0	0.0	0.0	0.0	2.8486903858123823E-4
8	7.121725964530956E-5	0.0	0.0	0.0	2.8486903858123823E-4
9	7.121725964530956E-5	0.0	0.0	0.0	2.8486903858123823E-4
10	7.121725964530956E-5	0.0	0.0	0.0	2.8486903858123823E-4
11	7.121725964530956E-5	0.0	0.0	0.0	2.8486903858123823E-4
12	7.121725964530956E-5	0.0	0.0	0.0	4.985208175171669E-4
13	7.121725964530956E-5	0.0	0.0	0.0	4.985208175171669E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTATA	75	9.705818E-7	13.9288645	2
CGTCTTA	65	5.444456E-5	13.155724	15
CCTTATA	210	0.0	13.114839	2
CCGTCGT	110	2.744855E-9	12.954546	9
CGAATGA	235	0.0	12.937093	14
ACCGTTA	60	4.0915835E-4	12.666667	8
CCGTTAT	60	4.0915835E-4	12.666667	9
TAGGACC	540	0.0	12.488959	4
CTCGTCT	70	1.0903913E-4	12.214721	13
CTTATAC	250	0.0	12.157399	3
GTCGTGG	55	0.0030813222	12.084016	1
ACTGTTC	300	0.0	12.033334	8
GCGTTAT	80	2.8826675E-5	11.86823	1
GCGAATG	265	0.0	11.830611	13
GACCGTT	65	8.0225634E-4	11.692308	7
CGACGTT	155	2.5465852E-11	11.645162	7
CCCTTAT	245	0.0	11.62602	1
AACCGCG	90	7.458997E-6	11.611112	7
CGTTATT	90	7.4856853E-6	11.607388	2
CGTCGTA	115	7.07023E-8	11.565217	10
>>END_MODULE