FastQCFastQC Report
Thu 2 Feb 2017
SRR4062529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404154
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA60020.427445992391148No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC48070.34234136711500307No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20990.14948502799550478No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC20950.1492001589569235No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG15900.11323544283604219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT454.070188E-529.33314311
TCGTATA856.114935E-620.70574844
TGCGCTA708.124568E-418.85702110
CCGAACG708.124568E-418.8570219
GTCCTAC7950.018.5453831
GATTTCG4650.018.45149241
TATGGCG8200.018.24378416
TGGTATA4250.018.1175344
TCGCGAT851.4311123E-418.11752943
ATTTCGT4650.017.97837842
CCTTATA3700.017.8383582
TAGGACC8150.017.8158364
GTCCTAA7950.017.7149931
TCCTACA9200.017.6961672
CCTTTAT3250.017.6042751
CGACACG750.001291067317.59988644
CGCACGA2250.017.59988642
TAGGACG6750.017.5998864
CCCTTAT4150.017.4982241
ATGGCGA8600.017.39523517
Produced by FastQC (version 0.11.5)