##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062529_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1404154
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.267210719052184	32.0	32.0	32.0	32.0	32.0
2	31.393780881584213	32.0	32.0	32.0	32.0	32.0
3	31.499490796593538	32.0	32.0	32.0	32.0	32.0
4	31.606801675599684	32.0	32.0	32.0	32.0	32.0
5	31.544547820253335	32.0	32.0	32.0	32.0	32.0
6	35.13899045261417	36.0	36.0	36.0	36.0	36.0
7	35.17193911778907	36.0	36.0	36.0	36.0	36.0
8	35.106635739384714	36.0	36.0	36.0	36.0	36.0
9	35.23887978099268	36.0	36.0	36.0	36.0	36.0
10	35.123226512191685	36.0	36.0	36.0	36.0	36.0
11	35.24996830831946	36.0	36.0	36.0	36.0	36.0
12	35.16445703249074	36.0	36.0	36.0	36.0	36.0
13	35.19981426538685	36.0	36.0	36.0	36.0	36.0
14	35.15557766455816	36.0	36.0	36.0	36.0	36.0
15	35.122326326029764	36.0	36.0	36.0	36.0	36.0
16	35.1365961283449	36.0	36.0	36.0	36.0	36.0
17	35.11575795817268	36.0	36.0	36.0	36.0	36.0
18	35.128918195582536	36.0	36.0	36.0	36.0	36.0
19	35.10103236539582	36.0	36.0	36.0	36.0	36.0
20	35.093365827395	36.0	36.0	36.0	36.0	36.0
21	35.07618751219596	36.0	36.0	36.0	36.0	36.0
22	35.04856091283435	36.0	36.0	36.0	36.0	36.0
23	34.987371043347096	36.0	36.0	36.0	36.0	36.0
24	34.96702284792124	36.0	36.0	36.0	36.0	36.0
25	34.9372340925568	36.0	36.0	36.0	32.0	36.0
26	34.891851606020424	36.0	36.0	36.0	32.0	36.0
27	34.86046046231396	36.0	36.0	36.0	32.0	36.0
28	34.8258773610302	36.0	36.0	36.0	32.0	36.0
29	34.8067419955361	36.0	36.0	36.0	32.0	36.0
30	34.77694113323752	36.0	36.0	36.0	32.0	36.0
31	34.76264498053632	36.0	36.0	36.0	32.0	36.0
32	34.72532784865478	36.0	36.0	36.0	32.0	36.0
33	34.70180478779393	36.0	36.0	36.0	32.0	36.0
34	34.68211820071018	36.0	36.0	36.0	32.0	36.0
35	34.625828078686524	36.0	36.0	36.0	32.0	36.0
36	34.58578902314134	36.0	36.0	36.0	32.0	36.0
37	34.539856739360495	36.0	36.0	36.0	32.0	36.0
38	34.48089383358236	36.0	36.0	36.0	32.0	36.0
39	34.382967252879666	36.0	36.0	36.0	32.0	36.0
40	34.32326368760122	36.0	36.0	36.0	32.0	36.0
41	34.32969318180199	36.0	36.0	36.0	32.0	36.0
42	34.21967319823894	36.0	36.0	36.0	32.0	36.0
43	34.23371724184099	36.0	36.0	36.0	32.0	36.0
44	34.215341764507315	36.0	36.0	36.0	32.0	36.0
45	34.09012259339075	36.0	36.0	36.0	32.0	36.0
46	34.15477362169676	36.0	36.0	36.0	32.0	36.0
47	34.089775053163685	36.0	36.0	36.0	32.0	36.0
48	34.09169720700151	36.0	36.0	36.0	32.0	36.0
49	34.10772465128469	36.0	36.0	36.0	32.0	36.0
50	33.567325948578286	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	13.0
21	35.0
22	105.0
23	349.0
24	989.0
25	2395.0
26	4941.0
27	9395.0
28	16198.0
29	24550.0
30	35442.0
31	50510.0
32	76693.0
33	114471.0
34	240386.0
35	827680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.59325760743288	17.8662932368798	11.622717200302295	25.917731955385015
2	15.329540647216094	20.19854626360118	38.288277380767546	26.183635708415174
3	19.16490106078788	24.759337532804658	28.825156946041897	27.25060446036556
4	12.064015907121101	16.140785428347808	37.36314684104487	34.43205182348622
5	13.287360218323633	37.6376807672093	34.46459576371253	14.610363250754546
6	32.84285657311501	36.55871477569252	17.23242060643353	13.366008044758942
7	28.903687845982596	30.838234864719162	21.336991054393646	18.921086234904603
8	27.693258716636493	34.53524328528068	19.59777916097522	18.17371883710761
9	27.28924320266865	14.33047942034848	18.50559126705475	39.87468610992811
10	15.348530146978181	27.59490768106632	32.93933571388894	24.117226458066565
11	37.0803344932251	21.371729881480235	22.153980261424316	19.393955363870344
12	22.82598632343746	24.24912082292968	30.572928610394584	22.351964243238275
13	30.11528649991383	20.394700296406235	25.38268594470407	24.107327258975868
14	22.90596330601914	20.04310068553734	25.553749802372106	31.497186206071415
15	25.7102853390725	27.497055166313665	23.39593805237887	23.39672144223497
16	25.337676636608236	26.321400644088893	25.080226242990438	23.26069647631243
17	23.561874267352444	26.31862317096273	26.619658527483452	23.499844034201377
18	24.30438541641444	25.099454903094674	28.27709781120874	22.319061869282145
19	25.153366368646175	26.62685147070763	25.2827681294217	22.937014031224496
20	24.432718918295286	24.77776654127681	27.515856522860027	23.273658017567875
21	25.493143914413945	25.18762187053557	24.654916768388652	24.664317446661833
22	23.968026299109642	26.304664588072246	25.41003337240787	24.317275740410242
23	23.21355065042723	25.908198103626813	26.05583148287154	24.82241976307442
24	23.556248103840463	26.77505458802952	25.60224875619056	24.06644855193946
25	24.796852766861754	25.174304242981894	25.146173425421996	24.882669564734353
26	23.161134747328287	25.51614708927938	26.421959414708073	24.90075874868426
27	25.256346526093292	25.946512989315984	25.70230900599222	23.0948314785985
28	23.791692364227856	25.159704704754603	25.990311603997853	25.05829132701969
29	23.685436212837054	25.816399055944007	26.333365143709308	24.164799587509634
30	24.310510100743933	25.77594765246547	26.585545460113348	23.327996786677243
31	23.95043563597725	25.251005231619892	26.013955734200096	24.784603398202762
32	23.6809495254794	25.97642423836702	25.79303979478034	24.54958644137324
33	23.45469229158625	25.503114330764287	26.265779964305906	24.77641341334355
34	23.318809760182997	26.198266002162157	26.979590557730848	23.503333679923998
35	24.696650082540806	25.570343423869463	26.331513494958532	23.401492998631205
36	23.738564288532455	26.196200701632442	26.190289669081878	23.87494534075322
37	24.806751965952454	25.61442690758991	25.7937519673768	23.78506915908084
38	24.122995056097835	26.09500097567646	25.474057688829006	24.307946279396706
39	24.521954144630858	25.26104686522988	26.095784365532555	24.1212146246067
40	25.054730464037423	25.75023822173351	25.379338733500738	23.815692580728324
41	23.65723417801751	25.416442925775947	26.709036188338313	24.217286707868226
42	25.518639693366968	26.259940149014994	25.48701923008445	22.734400927533592
43	24.462915036384896	24.779689407287233	25.683934953003728	25.073460603324136
44	23.776665522442695	25.549405549533738	26.926961002852963	23.7469679251706
45	24.596874701777725	25.495707735761176	25.933195361762312	23.974222200698783
46	24.015029690475544	25.132072408012228	26.74457360090133	24.1083243006109
47	24.729356416287967	24.788751653131815	26.718811981315426	23.763079949264785
48	23.774529004653335	27.23554538889609	25.19310559952826	23.796820006922317
49	23.534313187869706	25.380478209655067	27.30263204748197	23.782576554993256
50	23.19339616594761	27.329694606147186	24.8113810878294	24.665528140075804
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	20.0
2	25.0
3	98.5
4	172.0
5	198.5
6	225.0
7	220.0
8	215.0
9	287.0
10	359.0
11	518.0
12	677.0
13	1224.0
14	1771.0
15	2423.0
16	3075.0
17	3597.5
18	4120.0
19	4468.5
20	4817.0
21	5645.5
22	6474.0
23	7437.5
24	8401.0
25	9885.5
26	11370.0
27	14367.0
28	17364.0
29	19913.5
30	22463.0
31	25396.0
32	28329.0
33	33412.5
34	38496.0
35	43566.5
36	48637.0
37	53473.5
38	58310.0
39	60468.0
40	62626.0
41	67067.0
42	71508.0
43	76394.0
44	81280.0
45	93211.0
46	105142.0
47	113422.5
48	121703.0
49	119798.5
50	117894.0
51	112855.0
52	107816.0
53	103640.0
54	99464.0
55	95832.5
56	92201.0
57	85923.0
58	79645.0
59	71907.0
60	64169.0
61	55584.5
62	47000.0
63	39651.5
64	32303.0
65	27334.5
66	22366.0
67	18279.5
68	14193.0
69	12820.5
70	11448.0
71	8858.0
72	6268.0
73	5638.0
74	5008.0
75	3660.5
76	2313.0
77	2027.0
78	1741.0
79	1447.0
80	1153.0
81	916.0
82	679.0
83	562.0
84	445.0
85	359.0
86	273.0
87	203.0
88	133.0
89	87.0
90	41.0
91	25.0
92	9.0
93	9.0
94	9.0
95	6.0
96	3.0
97	4.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01645118697806651
2	0.003418428462974859
3	6.409553368077861E-4
4	2.1365177893592868E-4
5	0.0
6	7.121725964530956E-4
7	7.121725964530956E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	7.121725964530956E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1404154.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.978470975766704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74677321575186	44.64102429869015
2	12.526051189504047	14.023783859052404
3	3.3072336385346777	5.554016467343782
4	1.3492127952189787	3.0210747718919464
5	0.7192668567011893	2.013172943083924
6	0.4217944734426696	1.4166845813609568
7	0.2915162066269198	1.1423042058141444
8	0.21936951698343699	0.982397611154023
9	0.1596949345556372	0.8045530433099732
>10	1.0718520712674464	11.52607588982286
>50	0.11060290081266443	4.2886244791834764
>100	0.07152341934258163	7.554094707781685
>500	0.0038315859433901488	1.4442079049896888
>1k	0.0011494757830170445	1.1588696005929635
>5k	1.2771953144633829E-4	0.42911563592812435
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6002	0.427445992391148	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4807	0.34234136711500307	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2099	0.14948502799550478	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2095	0.1492001589569235	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1590	0.11323544283604219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	7.121725964530956E-5
6	0.0	0.0	0.0	0.0	7.121725964530956E-5
7	0.0	0.0	0.0	0.0	7.121725964530956E-5
8	0.0	0.0	0.0	0.0	7.121725964530956E-5
9	0.0	0.0	0.0	0.0	7.121725964530956E-5
10	0.0	0.0	0.0	0.0	7.121725964530956E-5
11	0.0	0.0	0.0	0.0	7.121725964530956E-5
12	0.0	0.0	0.0	0.0	1.4243451929061912E-4
13	0.0	0.0	0.0	0.0	2.1365177893592868E-4
14	0.0	0.0	0.0	0.0	2.1365177893592868E-4
15	0.0	0.0	0.0	0.0	2.8486903858123823E-4
16	0.0	0.0	0.0	0.0	3.560862982265478E-4
17	0.0	0.0	0.0	0.0	3.560862982265478E-4
18	0.0	0.0	0.0	7.121725964530956E-5	3.560862982265478E-4
19	0.0	0.0	0.0	7.121725964530956E-5	3.560862982265478E-4
20	0.0	0.0	0.0	2.8486903858123823E-4	3.560862982265478E-4
21	0.0	0.0	0.0	5.697380771624765E-4	3.560862982265478E-4
22	0.0	0.0	0.0	9.258243753890242E-4	3.560862982265478E-4
23	0.0	0.0	0.0	0.0018516487507780485	3.560862982265478E-4
24	0.0	0.0	0.0	0.0028486903858123826	3.560862982265478E-4
25	0.0	0.0	0.0	0.004273035578718574	3.560862982265478E-4
26	0.0	0.0	0.0	0.005768598031270074	3.560862982265478E-4
27	0.0	0.0	0.0	0.00790511582062936	3.560862982265478E-4
28	0.0	0.0	0.0	0.012178151399347935	3.560862982265478E-4
29	0.0	0.0	0.0	0.018445270248135175	3.560862982265478E-4
30	0.0	0.0	0.0	0.031406811503581517	3.560862982265478E-4
31	0.0	0.0	0.0	0.05533581074440553	3.560862982265478E-4
32	0.0	0.0	0.0	0.08652897046905111	3.560862982265478E-4
33	0.0	0.0	0.0	0.11508709158682025	3.560862982265478E-4
34	0.0	0.0	0.0	0.15404293261280458	3.560862982265478E-4
35	0.0	0.0	0.0	0.1973430264771528	3.560862982265478E-4
36	0.0	0.0	0.0	0.2573791763581488	3.560862982265478E-4
37	0.0	0.0	0.0	0.3525254352442823	3.560862982265478E-4
38	0.0	0.0	0.0	0.46818226490826503	3.560862982265478E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTAT	45	4.070188E-5	29.333143	11
TCGTATA	85	6.114935E-6	20.705748	44
TGCGCTA	70	8.124568E-4	18.857021	10
CCGAACG	70	8.124568E-4	18.857021	9
GTCCTAC	795	0.0	18.545383	1
GATTTCG	465	0.0	18.451492	41
TATGGCG	820	0.0	18.243784	16
TGGTATA	425	0.0	18.11753	44
TCGCGAT	85	1.4311123E-4	18.117529	43
ATTTCGT	465	0.0	17.978378	42
CCTTATA	370	0.0	17.838358	2
TAGGACC	815	0.0	17.815836	4
GTCCTAA	795	0.0	17.714993	1
TCCTACA	920	0.0	17.696167	2
CCTTTAT	325	0.0	17.604275	1
CGACACG	75	0.0012910673	17.599886	44
CGCACGA	225	0.0	17.599886	42
TAGGACG	675	0.0	17.599886	4
CCCTTAT	415	0.0	17.498224	1
ATGGCGA	860	0.0	17.395235	17
>>END_MODULE