FastQCFastQC Report
Thu 2 Feb 2017
SRR4062528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255887
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG36990.16397097904283325No Hit
GTATCAACGCAGAGTACTTTTTTTT30960.1372409167657777No Hit
TCCATGTACTCTGCGTTGATACCAC30130.13356165446230242No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27670.12265685293633946No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27450.12168162678361107No Hit
GAATAGGACCGCGGTTCTATTTTGT24970.11068816833467278No Hit
GAATAACGCCGCCGCATCGCCAGTC24750.1097129421819444No Hit
GCGCAAGACGGACCAGAGCGAAAGC24310.1077624898764876No Hit
TATCAACGCAGAGTACTTTTTTTTT22690.1005812791154876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1900.013.9905351
CGTTATT1850.013.8561082
TAGAGTG2800.013.228485
GTTATTC2300.012.7976793
ACGGTAT4700.012.7358879
CAAGACG5050.012.5996324
CGCGTAA1600.012.47055710
GAGCGTT1155.318725E-912.3928257
AACCGCG2150.012.37361057
CCGACCA3850.012.339459
AAGACGG5350.012.2489455
CGCATCG4350.012.23111313
TTAGAGT3200.012.1676954
TACACCG1103.8315193E-812.0875635
GTCCTAA1103.8529834E-812.0827361
CGTCTTA1900.012.00173915
ATCGCCA4800.011.87777616
CGACCAT3850.011.84587210
TCGCCAG5100.011.73881817
GAACCGC2600.011.6932236
Produced by FastQC (version 0.11.5)